Doctor of Philosophy, Stanford University, MI-PHD (2013)
Bachelor of Science, University of California Los Angeles, Microbiology and Immunology (2008)
Previous work has proposed rhoptry protein 2 (ROP2) as the physical link that tethers host mitochondria to the parasitophorous vacuole membrane (PVM) surrounding the intracellular parasite, Toxoplasma gondii. A recent analysis of the ROP2 structure, however, raised questions about this model. To determine whether ROP2 is necessary, we created a parasite line that lacks the entire ROP2 locus consisting of the three closely related genes, ROP2a, ROP2b and ROP8. We show that this knockout mutant retains the ability to recruit host mitochondria in a manner that is indistinguishable from the parental strain, re-opening the question of which molecules mediate this association.
View details for DOI 10.1016/j.ijpara.2010.07.002
View details for Web of Science ID 000282867200002
View details for PubMedID 20637758