Technologies used in the lab
What we do
We use xMAP technology by Luminex to detect cytokine expression and secretion in lymphocyte samples from mouse and human sources. With the available technology we can detect over 60 different cytokines in a sample as small as 10 ul. We are interested in cytokine expression in human diseases, before and after immunization and in the healthy general population. We also study various mouse models of autoimmune diseases.
This multiplexed cytokine assay allows generation of large amounts of data and therefore new analyses which result in new insights into the roles of cytokines in health and disease.
How this works
Luminex color-codes tiny beads, called microspheres, into (potentially) 100 distinct sets. Each bead set is then coated with an antibody to a specific cytokine or chemokine allowing the capture and detection of specific analytes from a sample. Within the Luminex compact analyzer, lasers excite the internal dyes that identify each microsphere particle, and also any reporter dye captured during the assay. Many readings are made on each bead set, further validating the results. In this way, xMAP technology allows multiplexing of up to 100 unique assays within a single small volume sample, both rapidly and precisely.