Timeline and Guideline for users of room Sim1 G0809

Following rederivation or IVF, recipient mothers are housed in quarantine room Sim1 G0809.  Here is a timeline of what to expect.

  1. Once your rederivation/IVF is scheduled, you will need to order a sheet of cage card stickers through animaltrax to be sent to Sim1 room 809 if you have not done so already.
  2. On the day of rederivation/IVF, you will be updated on the rederivation by TKTC.
  3. Following rederivation, TKTC will care for and monitor the embryo recipient mothers weekly.  This includes removing wound clips, separating co-housed recipient mothers, and housing them with appropriate nesting material.   Tissue collection for genotyping can be requested for an additional fee.  Please contact TKTC before the pups are born, if you are interested in this procedure.
  4. After the pups are born (approximately 19 days after IVF) TKTC will notify you with the pup birth rates and an approximation of weaning date (approximately 21 days after birth). 
  5. Once the pups are weaned, TKTC will notify you with detailed information on recipient mothers, the newly weaned pups, and the corresponding cage card numbers.  At this time, you will need to request pathogen testing from VSC.  Pathogen testing is taken place on surrogate mother(s), it can take at least 2 weeks. 
  6. Once pathogen tests results return negative, you will be notified by VSC to transfer the weaned pups to your animal room.  Any remaining recipient mothers will be culled at this time.

How to order cage card stickers through animaltrax

  1. Go to animaltrax.stanford.edu
  2. Click on eAnimal ordering, investigator, and scroll down to splitting and weaning.
  3. At the bottom of the page, check the appropriate available protocol.  Then click on the Split/Wean request blue button.
  4. Complete all the necessary fields.  The building choice is Sim1.  The room choice is G0809.  Once this is complete, click on the blue Submit button.

How to request pathogen testing

  1. Go to this link to request pathogen testing: http://vsc.stanford.edu/forms/post-rederivation.html
  2. Complete all the required fields.  TKTC will provide you with the DOB, wean date, Embryo recipient barcode and corresponding pup barcodes.  In the comments section, please let VSC know that all the recipients were housed together for at least 12 days prior to being separated for birthing.  The recipient mother that is sent for testing is representative of all the remaining recipient mothers and their pups for this strain rederivation. 
  3. Submit your request.

How to transfer mice to your animal room from 809

  1. Go to animaltrax.stanford.edu
  2. Click on eAnimal ordering, investigator, animal transfer
  3. Click on “Internal” for internal transfers within Stanford. Click New Transfer and complete requested information, building choice is Sim1, room choice is G0809. The info of re-derived mouse DOB and cage barcodes is in TKTC’s notification.
  4. Click on Submit. 









All services need to be requested through iLab



Prof. Vittorio Sebastiano, PhD

Assistant professor of Obstetrics & Gynecology





Dr. Hong Zeng, MD, PhD

Contact for genome editing and mouse line services




Research Scientist, Tumor model group leader

Nirk Quispe Calla, MD

Contact for tumor model services