Pathway of care for visual and vestibular rehabilitation after mild traumatic brain injury: a critical review.
To review the pathway to care for treatment and management of patients receiving visual and vestibular rehabilitation after mild traumatic brain injury (mTBI).English scientific peer-reviewed articles from PubMed, CINAHL, Embase, and PsycINFO between 2000 and 2020 were first screened by title and abstract, then those selected underwent full-text review and analysis.The database search yielded 1640 results and after title and abstract review, 75 articles were selected for full-text screening, from which 8 were included in the qualitative synthesis. Current evidence includes a limited number of retrospective cohort studies and case studies.Many patients with visual and vestibular deficits following mTBI do not receive rehabilitation services until months following their injury as there is no standardized pathway to care for patients for visual and vestibular rehabilitation. Barriers to establishing a standardized pathway are the lack of natural history data for visual and vestibular function following mTBI and the lack of randomized clinical trials establishing the efficacy of rehabilitation in patients following mTBI.
View details for DOI 10.1080/02699052.2022.2105399
View details for PubMedID 35918848
The Use of Panitumumab-IRDye800CW in a Novel Murine Model for Conjunctival Squamous Cell Carcinoma.
Translational vision science & technology
2022; 11 (7): 23
Purpose: Conjunctival squamous cell carcinoma (SCC) is a sight-threatening ocular surface malignancy with the primary treatment modality being surgical resection. To evaluate surgical imaging modalities to improve surgical resection, we established a novel murine model for conjunctival SCC to demonstrate the utility of panitumumab-IRDye800, a fluorescently labeled anti-epidermal growth factor receptor (EGFR) antibody.Methods: NOD-scid IL2Rgammanull (NSG) mice received subconjunctival injection of UM-SCC-1 or SCC-9, head and neck SCC cell lines. On tumor growth, mice were injected with Panitumumab-IRDye800CW, and imaged with a small animal imaging system and optical coherence tomography (OCT). Immunohistochemistry for SCC markers were used to confirm tumor origin.Results: Seventy-five percent (N = 4) of the UM-SCC-1 group developed aggressive, rapidly growing tumors that were P40 and EGFR positive within two weeks of inoculation. The SCC-9 tumors failed to demonstrate any growth (N = 4). Ocular tumors demonstrated high fluorescence levels with a tumor to background ratio of 3.8.Conclusions: Subconjunctival injections are an appropriate technique to create in vivo models for assessing treatment modalities and novel therapies in conjunctival SCC.Translational Relevance: This model demonstrates Panitumumab-IRDye800CW's utility in the ophthalmic setting and suggests that clinical trials may be warranted.
View details for DOI 10.1167/tvst.11.7.23
View details for PubMedID 35895055
The Tabula Sapiens: A multiple-organ, single-cell transcriptomic atlas of humans.
Science (New York, N.Y.)
2022; 376 (6594): eabl4896
Molecular characterization of cell types using single-cell transcriptome sequencing is revolutionizing cell biology and enabling new insights into the physiology of human organs. We created a human reference atlas comprising nearly 500,000 cells from 24 different tissues and organs, many from the same donor. This atlas enabled molecular characterization of more than 400 cell types, their distribution across tissues, and tissue-specific variation in gene expression. Using multiple tissues from a single donor enabled identification of the clonal distribution of T cells between tissues, identification of the tissue-specific mutation rate in B cells, and analysis of the cell cycle state and proliferative potential of shared cell types across tissues. Cell type-specific RNA splicing was discovered and analyzed across tissues within an individual.
View details for DOI 10.1126/science.abl4896
View details for PubMedID 35549404
- Publisher Correction: Cell types of origin of the cell-free transcriptome. Nature biotechnology 2022 Hide More
- Sentiment Analysis Surrounding Blepharoplasty in Online Health Forums PLASTIC AND RECONSTRUCTIVE SURGERY-GLOBAL OPEN 2022; 10 (3) Hide More
Sentiment Analysis Surrounding Blepharoplasty in Online Health Forums.
Plastic and reconstructive surgery. Global open
2022; 10 (3): e4213
Upper and lower blepharoplasty are among the most common procedures in aesthetic surgery and are often emotionally laden due to the subjective nature of outcomes and implications with beauty and self-identity. This article capitalizes on the increasing wealth of patient-provided health information online and is the first to analyze the emotions surrounding blepharoplasty discussions in an open internet health forum, MedHelp.We used Python to scrape MedHelp for threads that contained "blepharoplasty" and then used IBM Watson Natural Language Understanding to perform sentiment analyses, calculating a general sentiment score (-1 to +1) as well as emotion scores for anger, sadness, joy, fear, and disgust (0 to 1) for posts and keywords contained within the posts. Keywords were then manually grouped into five distinct clinical categories: symptoms, doctor, treatment, medication, and body.We collected 52 threads containing "blepharoplasty," yielding 154 posts and 1365 keywords. The average sentiment score was negative among all posts (-0.15) and keywords (-0.30). Among all posts and keywords, sadness had the highest score and disgust had the lowest score.Fear and sadness are the predominant emotions for blepharoplasty patients online, and the most negative symptoms cited are not ones that surgeons typically expect.
View details for DOI 10.1097/GOX.0000000000004213
View details for PubMedID 35492229
View details for PubMedCentralID PMC9038503
Cell types of origin of the cell-free transcriptome.
Cell-free RNA from liquid biopsies can be analyzed to determine disease tissue of origin. We extend this concept to identify cell types of origin using the Tabula Sapiens transcriptomic cell atlas as well as individual tissue transcriptomic cell atlases in combination with the Human Protein Atlas RNA consensus dataset. We define cell type signature scores, which allow the inference of cell types that contribute to cell-free RNA for a variety of diseases.
View details for DOI 10.1038/s41587-021-01188-9
View details for PubMedID 35132263
PNP Hydrogel Prevents Formation of Symblephara in Mice After Ocular Alkali Injury.
Translational vision science & technology
2022; 11 (2): 31
Purpose: To create an alkali injury symblephara mouse model to study conjunctival fibrosis pathophysiology and test polymer nanoparticle (PNP) hydrogel as a preventative therapeutic.Methods: Mice were injured using NaOH-soaked filter paper to determine the optimal NaOH concentration to induce the formation of symblephara. Injured mice were observed for 7 days to detect the formation of symblephara. Forniceal shortening observed on hematoxylin and eosin (H&E)-stained tissue sections was used as a symblephara marker. Alpha-smooth muscle actin (alpha-SMA) expression, Masson's trichrome assay, and periodic acid-Schiff (PAS) staining were used to determine myofibroblast expression, collagen deposition, and goblet cell integrity. PNP hydrogel, with multivalent, noncovalent interactions between modified biopolymers and nanoparticles, was applied immediately after alkali injury to determine its ability to prevent the formation of symblephara.Results: Forniceal shortening was observed in H&E images with 1N NaOH for 2 minutes after 7 days without globe destruction. PNP hydrogel prevented forniceal shortening after alkali injury as observed by H&E histology. alpha-SMA expression and collagen deposition in eye tissue sections were increased in the fornix after injury with 1N NaOH compared with uninjured controls. PNP hydrogel treatment immediately after injury reduced alpha-SMA expression and collagen deposition in the forniceal region. Mucin-secreting goblet cells stained with PAS were significantly lower in alkali-injured and PNP hydrogel-treated conjunctivas than in uninjured control conjunctivas.Conclusions: We observed that 1N NaOH for 2 minutes induced maximal forniceal shortening and symblephara in mice. PNP hydrogel prevented forniceal shortening and conjunctival fibrosis after injury. This first murine model for symblephara will be useful to study fibrosis pathophysiology after conjunctival injury and to determine therapeutic targets for cicatrizing diseases.Translational Relevance: This mouse model of symblephara can be useful for studying conjunctival scarring disease pathophysiology and preventative therapeutics. We tested PNP hydrogel, which prevented the formation of symblephara after injury.
View details for DOI 10.1167/tvst.11.2.31
View details for PubMedID 35191963
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Kim JY, Park J, Kim DH, Gardner B, Jenkins S, McGahan PJ, Chen JL. Revision Anterior Cruciate Ligament Reconstruction: Tibial Tunnel First Graft Sizing Technique. Arthrosc Tech. 2021 Nov 29;10(12):e2797-e2803. doi:10.1016/j.eats.2021.08.017
Kim JY, Park HY, Del Sol SR, Bryant S, Gardner B, Chakrabarti MO, Perinovic S, McGahan PJ, Chen JL. Arthroscopic Single Portal, Single Anchor Knotless Subscapularis Repair with Concomitant Tenodesis of the Long Head of the Biceps Tendon. Arthrosc Tech. 2021 Mar 22;10(4):e1117-e1123. doi: 10.1016/j.eats.2021.01.002. PMID: 33981559; PMCID: PMC8085439.
Kim JY, Park HY, Bryant S, Gardner B, Chakrabarti M, McGahan P, Chen JL. Combined Coracoclavicular and Acromioclavicular Joint Reconstruction with Allograft Using a Cerclage Tensioning System. Arthrosc Tech. 2021 Jan 16;10(2):e317-e323. doi: 10.1016/j.eats.2020.10.013. PMID: 33680762; PMCID: PMC7917031.
Kim JY, Bryant S, Gardner B, Park HY, Tse W, Chakrabarti M, McGahan P, Chen JL. Distal Clavicle Excision for Acromioclavicular Joint Osteoarthritis Using a Fluoroscopic Kirschner Wire Guide. Arthrosc Tech. 2021 Jan 16;10(2):e359-e365. doi: 10.1016/j.eats.2020.10.010. PMID: 33680767; PMCID: PMC7917026.
Park HY, Gardner B, Kim JY, Bryant S, Chakrabarti M, McGahan P, Chen JL. Four-Strand Hamstring Diamond Braid Technique for Anterior Cruciate Ligament Reconstruction. Arthrosc Tech. 2021 Mar 22;10(4):e1173-e1177. doi: 10.1016/j.eats.2021.01.011. PMID: 33981567; PMCID: PMC8085571.
Bryant SA, Trang G, Nash HM, Gardner BB, Kim JY, Park HY, Chakrabarti M, McGahan P, Chen JL. Mini-Open Achilles Repair With a Flat Braided Suture in a Low-Profile Configuration. Arthrosc Tech. 2021 Jan 16;10(2):e451-e455. doi: 10.1016/j.eats.2020.10.019. PMID: 33680778; PMCID: PMC791708
Nguyen AX, Ratan S, Biyani A, Trinh XV, Saleh S, Sun Y, Wu AY. Gender of Award Recipients in Major Ophthalmology Societies. Am J Ophthalmol. 2021 Nov;231:120-133. doi: 10.1016/j.ajo.2021.05.021. Epub 2021 Jun 6. PMID: 34102152; PMCID: PMC8608693.
Nguyen AX, Yoffe L, Li A, Trinh XV, Kurian J, Moss HE, Wu AY. Gender Gap in Neurology Research Authorship (1946-2020). Front Neurol. 2021 Aug 23;12:715428.
Sanyam Ratan, Anne Xuan-Lan Nguyen, Ankita Biyani, Xuan-Vi Trinh, Yang Sun, Solin Saleh, Albert Y. Wu; Gender Gap in the Award Recipients of the Association for Research in Vision and Ophthalmology. Invest. Ophthalmol. Vis. Sci. 2021;62(8):65.