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The van de Rijn Lab
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In The Department of Pathology
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Protocols
Histology
Tissue Microarray
VECTASTAIN Elite ABC Kit
Immunostaining-DAKO Envision Plus Kits
Hematoxylin and Eosin Stain for Paraffin Sections
Double Immunofluorescence Protocol
Single Immunofluorescence Protocol
Molecular Biology
RNA
mRNA Isolation and Purification for cDNA Microarray
Total RNA Isolation and Purification for cDNA Microarray
Total RNA Isolation From Formaldehyde Fixed Paraffin-Embedded Tissues (FFPE)
DNase Treatment
RNA Amplification
DNA
DNA Extraction from the Organic Phase of Trizol Reagent after RNA Isolation
DNA Isolation from Frozen Tissue
DNA Extraction from Formaldehyde Fixed Paraffin-embedded Tissue (FFPE)
cDNA library construction
3 end RNAseq Library Construction for Illumina HISeq
mRNA Isolation for cDNA Library Construction
Fluorescence In Situ Hybridization
DNA isolation from BAC
Labeling of BAC Clones
Paraffin FISH Protocol
FISH for Metphase Spread
RNA probes for In Situ Hybridization
Non-radioactive In Situ Hybridization of Paraffin Embedded Tissues
Western Blot
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