Stanford Core Services
Proteomics, Genomics and Imaging Resources for Translational Research @ Stanford
- Gene Expression Analysis: Microarrays (Affymetrix), Real-time PCR, Pyrosequencing (Quantification and CpG methylation and genetic alterations), Nucleic Acid QC (Bioanalyzer).
- DNA Sequencing
- Oligonucleotide sequencing
- Next-Generation Sequencing
- Biomolecular Interaction Analysis (Surface Plasmon Resonance – Biacore)
- Protein Sequencing
- Peptide Synthesis
- Protein Identification
- Mass Spectrometry
- Multiplex Luminex assays for cytokines
- Immunophenotyping by flow cytometry
- Phosho-epitope flow cytometry
- RNA/DNA extraction and genomic assays
- Serum and PBMC preparations and banking
- CyTOF mass cytometry phenotyping assay
- High-throughput sequencing and library preparation for Illumina MiSeq, MiSeq, GAIIx, and HiSeq2000
- Microarrays processing utilizing Illumina, Affymetrix, Agilent, Nimblegen, Stanford and other commercial and custom microarrays
- Antibody phenotyping microarrays
- Microarray production for any type of biological material (cDNA, oligonucleotides, antibodies, peptides, cells, lysates)
- 24/7 access to equipment in the lab
- Reagents: Antibodies, Open Biosystem products, and doping controls
- Proteomics: Protein identification, post-translational and synthetic modification, global proteome analysis, differential analysis and quantitative proteomics – labeled and label-free.
- Quantitative applications and assays: Quantitation of analytes in biological matcies, pharmacokinetic studies and PK assessment, protein and peptide quantification and biomarker verification.
- Metabolomics and drug metabolism: Primary metabolite profiling, metabolite identification, small molecule biomarker discovery and identification, lipid profiling and structural analysis.
- General analysis: Mass determination, MS/MS, MSn, analytical scale LC-UV-MS, high resolution accurate mass MS (molecular formula confirmation)
- Open access lab: LC-MS, GC-MS
- Consulation, experimental design, education and informatics support for data analysis.
- Custom projects: Method development, protein folding, non-covalent interactions.
- Fluorescence microscopy services: Training and consultation in the use of confocal, deconvolution, and multi-photon for multidimentional (3D, 4D) imaging of fixed and live cell tissue samples
- Electron microscopy services: Full service laboratory with sample preparation and training for both transmission and scanning electron microscopy technologies
- Data management includes server storage and tape data archiving.
- Small animal imaging instrumentation for both structural and functional analyses with the capability of in vivo optical bioluminescence and fluorescence imaging, MRI, MicroCT, MicroPET, MicroSPECT, Ultrasound and Photo-acoustic imaging
- Training and education in imaging of cellular and molecular processes for high quality image data
- Reagents and support services for optimal in vivo analysis.
- Data handling: 14-terabyte storage system with both RAID and tape backup.
Computational Resources for Translational Research @ Stanford
Philip W. Lavori PhD
Professor of Biostatistics and Statistics, and Chair, Dept. of Health Research and Policy, School of Medicine
Redwood Bldg T152a
Area of expertise: Clinical research (especially trials)
Nigam Shah, MBBS, PhD
Assistant Professor of Medicine (biomedical informatics
Center for Biomedical Informatics Research
Area of expertise: Methods to analyze large unstructured data sets for data-driven medicine: medical informatics, knowledge representation, unstructured data-mining, ontologies.
Stanford Center for Biomedical Informatics Research
Quantitative Sciences Unit, Dept. of Medicine
Other Core Resources for Translational Research
- Flow Cytometry:
Stanford Shared FACS Facility
- Clinical Specimen Collection, Processing and Banking; Histological Staining and Pathological Review:
- High-throughput Screens for the Purpose of Drug and/or Target Discovery:
Stanford High-Throughput Bioscience Center
- Clinical Trials:
Stanford Center for Clinical and Translational Education and Research
- IRB Protocol Assistance:
Stanford Research Compliance Office: Human Subjects Research