- Charge (NIA) and Size-Based (SimpleWestern) Protein Separation Nano ImmunoAssay [ProteinSimple: PeggySue]:
- Assay development and optimization, set-up, data analysis
- Sample processing:
- DNA/RNA/protein isolation from tissue, cells, paraffin block sections
- Blood processing for NIA (PBMC isolation, serum, plasma)
- Cell culture services:
- Culturing primary cells and cell-lines
- Assay development for detection and validation of Circulating Tumor Cells (CTCs) from blood.
- Cryopreservation and sample banking
- Laboratory use for clinical investigators
- Technical consulting/individual training
Clinical Samples must be delivered to the TASC facility by 2:30pm for same day processing. After 2:30pm samples can be processed by special arrangement only, however after-hours fees may apply. Please contact TASC director for more information.
COVID related guidelines in the TASC Facility
DO NOT ENTER the TASC LAB (Grant S107, S106, S105) WITHOUT A PRIOR CONFIRMED APPOINTMENT WITH THE LAB MANAGER, SHAILJA PATEL (BARODA@STANFORD.EDU) OR TASC DIRECTOR, JOANNA LILIENTAL (JLILI@STANFORD.EDU)!!!
Guidelines within the lab:
• STARS COVID training (EHS-2470) needs to be completed before entering the facility.
• The Health Check needs to be completed before entering the facility.
• There should be no more than one person present in each room of the lab at any given time.
o If staff or another researcher is present, you MUST maintain 6 feet separation.
• The door to each sub-room of TASC must be closed when occupied (S107, S106).
• Wipe all surfaces (keyboard, mouse, desktop, PeggySue/Milo/centrigute/TissueLyser control panel buttons), with 70% before leaving.
• Wear a facial covering at all times in the lab – non-staff members must arrive at the facility with their face covering in place (staff will not be providing these). If you do not have a face covering, you will be asked to leave the facility.
• Do NOT enter the facility if you at all feeling ill, or if you currently have, or have had a fever in the last 14 days.
• All staff-assisted appointments will be contact-free drop-off only.
o Users must comply with the Sample Drop-Off procedure and leave samples at the designated spot in the facility (on the bench in Grant S106 lab).
• Shared PPE (face shields used for emptying waste containers) should be decontaminated by spraying with 70% alcohol after every use.
• Use red containers for waste
• Wipe down/disinfect work area after finishing use
Sample drop-off procedures:
CLINICAL SAMPLES, REAGENTS (ANTIBODIES, ETC) DROPPED OFF AT GRANT S106 AND PLACED INSIDE A STYROFOAM BOX ON TOP OF A WORKBENCH (WITH A NOTE ‘TASC’ ON TOP OF THE BOX. INSIDE THE BOX (WHICH SHOULD HAVE EITHER ICE OR DRY ICE, A NOTE INDICATING
I) NAME OF THE PERSON DROPPING OFF,
2) DATE AND TIME OF DROP OFF,
3) CONTENTS OF THE BOX,
4) INSTRUCTIONS FOR TASC.
THESE NOTES ALSO NEED TO BE SENT BY EMAIL TO SHAILJA PATEL AND JOANNA LILIENTAL.
REQUIRED PPE Use at TASC (you must bring your own supply before coming to TASC):
- Disposable gloves (Bring your own box with disposable gloves). After cleaning and before exiting the TASC lab, dispose of gloves and immediately wash hands with soap and water.
- Face mask that covers your mouth and nose.
If you use or touch any of these, you must clean it after each use and before exiting the TASC Lab:
- Chairs, computers (desktops, keyboards, monitors), light switches, door knobs, refrigerator/freezer doors and handles.
- Work bench tops
- Equipment panels/switches, instrument keyboards (PeggySue, Milo, Centrifuges, TissueLyzer control panel and rotor, water bath temperature controls)
- Biosafety cabinet and tissue culture hood sashes and their working surfaces
- Biowaste container lids
- Hand pipettors
- Outside of a pipette tip box that you have touched/used.
How to Properly Clean and Disinfect Surfaces at TASC:
- Spray bottle marked "70% alcohol" for disinfecting surfaces will be provided by TASC for your use on every work bench. Please notify TASC staff is the disinfectant volume is low.
- DO NOT spray disinfectant products directly onto surfaces, as this could lead to aerosolization of the virus. Spray disinfectant onto disposable paper towels to clean surfaces.
- At a minimum, disinfect at the end of each assay/procedure and before exiting the lab.
- Waste Disposal Items used for cleaning can be disposed of in regular trash. Excess waste from 70% alcohol should be disposed of as chemical waste appropriately,