Building technology to monitor alternative splicing on a genomic scale
This program investigates mRNA splicing patterns that differ in a systematic way between similar cells living in different environments. De-convoluting cellular splicing patterns will lead to a better understanding of the mechanism of pre-mRNA splicing and could provide a novel means for detecting, characterizing and potentially treating human diseases.
The two main focuses of this program are to:
- develop an accurate, quantitative, high-throughput assay for detecting small changes in splicing between two tissues on a genomic scale using MIPs and
- build a methodology to map processed introns in human cell lines using DNA microarrays.