The Chromium X instrument offers compatibility with an expanding range of Chromium Single Cell applications. This includes Single Cell Gene Expression Flex kits, which enable the most sensitive whole transcriptome profiling of fresh, frozen, and FFPE samples.
Scheduling Your Experiment - The single cell service is by appointment only. You must have an appointment prior to submitting samples. Submission appointments should be made a minimum of one week in advance of your anticipated submission date. This prevents scheduling conflicts and ensures the appropriate reagents are available and at the correct temperatures for immediate use. We will make effort to accommodate appointments made 24-48 hours before submission, but we cannot guarantee availability.
10X Capture Sample Preparation Considerations:
- Capture 1,000 - 10,000 cells with capture rate of ~65% (NextGEM)
Capture 1,000 - 20,000 cells with capture rate of ~70-80% (GEM-X)
- Flow sort prior to capture, if possible (isolate particular cell types, remove dead cells)
- Bring GRC single cell suspension with viability >70%
New 10x Products now available!
GEM-X:
- Higher Throughput (up to 20,000 cells versus 10,000 for NextGEM)
- Increased Sensitivity (two-fold increase in detected genes, detection of rare transcripts)
- Improved Recovery (Highly viable cells can have a capture rate of up to 80%)
FLEX:
- Profile gene expression for thousands to hundreds of thousands of cells or nuclei with a sensitive probe based method that captures the whole human or mouse transcriptome to detect even low-expressing genes
- Store samples without losing data quality, allowing you to batch samples in the same run and minimize experimental variability
- Fix fresh samples at collection to lock in biological states and preserve fragile cells
- Reduce experimental variability and increase efficiency by batching and multiplexing samples
- Probe-based method for human and mouse. Custom probe options available