Sequencing

Illumina Sequencing Services

 

Our facility currently offers Illumina NGS sequencing services on the platforms listed below, providing support and guidance from library construction through sequencing and overall experimental design to help you choose the most suitable platform for your project.

To set up a consultation for a project, or if you have any questions, please contact us at microarray@stanford.edu

 

 

NovaSeq X Plus

The latest high-throughput system, utilizing advanced XLEAP-SBS chemistry and integrated DRAGEN bioinformatics for superior speed and accuracy. It features 1.5B, 10B, and 25B patterned flow cells, capable of producing up to 16 Tb of data per run (with two flow cells running simultaneously).

 

 

 

NovaSeq 6000

The Illumina NovaSeq 6000 is a high-end sequencer designed to provide some of the highest throughput in the market. It has the capacity to run up to two flow cells at a time, with 2 flow cell options to choose from. The highest throughput (S2) can process up to 3.3 billion reads and generate up to 1000 Gb of data (with 150 bp read lengths). The system is compatible with Illumina library preparation kits, and can support virtually any sequencing application- whole genome sequencing, whole transcriptomes, whole exomes, metagenomics, and more.

 

 

NextSeq 2000 

Newest short-read sequencer, and has replaced the NextSeq 550.

Powered by XLEAP-SBS chemistry, a faster, higher quality, and more robust SBS chemistry. This delivers significant increases in sequencing reads and total output.

Four types of flow cell options are available with a range of outputs to accommodate a wide variety of NGS projects. The P4 is a high-output flow cell that generates up to 1.8 billion reads per run. At 100 million reads per run, the P1 flow cell is an attractive option for viral and pathogen sequencing, “smaller” batch RNA-Seq, targeted panels, and more.

MiSeq

The Illumina MiSeq System offers capabilities for small whole-genome sequencing, amplicon sequencing, gene expression profiling, small RNA analysis and 16s metagenomic sequencing in a compact instruement. Using a high throughput kit, MiSeq can produce as much as 15 Gb of output from 50 million paired end reads (at a read length of 300 bp).

 

MiSeq i100

The MiSeq i100 provides results 4× faster than the MiSeq System, with sequencing run times as fast as four hours.

With the expanded output offered by the MiSeq i100 Series, you can increase sample throughput, perform deeper sequencing, and gain more insights for a wide range of applications faster than ever. XLEAP-SBS chemistry on the MiSeq i100 Series eliminates thawing requirements so reagents can be used whenever your sample is ready.

 

 

 

iSeq 100

The iSeq 100 Sequencing System makes next-generation sequencing easier and more affordable than ever. Designed for simplicity, it allows labs of all sizes to sequence DNA and RNA at the push of a button. The iSeq 100 System is ideal for small whole-genome sequencing (eg, bacteria, viruses, plasmids), targeted sequencing of a set of genes or gene regions, gene expression analysis, or 16S metagenomics.

Complete Genomics Ultra-High Throughput: DNBSEQ-T7

Our MGI DNBSEQ-T7 covers a wide range of throughput read pairs with cost effectiveness. The ultra high-speed T7 can run 1 to 4 flow cells independently (each generating up to 6 billion read pairs depending on library type). 

Oxford Nanopore Sequencing Service

MinION

MinION is the only portable, real-time device for DNA and RNA sequencing, putting you in control of your sequence data. Each consumable flow cell can now generate as much as 30 Gb of DNA sequence data or 7-12 million reads if you are analysing RNA. Ultra-long read lengths are possible (hundreds of kb) as you can choose your fragment length. The MinION streams data in real time so that analysis can be performed during the experiment and workflows are fully versatile.

PacBio Revio sequencing

PacBio Revio

The PacBio Revio system improves upon the capabilities of the Sequel ll and Sequel lle by providing higher throughput, shorter turnaround time, reduced cost per Gb and higher compute power. The Revio system uses a high-density, 25 million ZMW SMRT Cell, up to 4 SMRT Cells in parallel sequencing to provide 360 Gb of HiFi reads per day. 

 

Benefits:

  • HiFi reads will have read lengths of ~15 kb to 18 kb 
  • Exceptional accuracy, quality of Q30 or better (99.999% consensus accuracy)
  • Data processed on the sequencer
  • DNA sample read data can include the 5mC methylation information
  • Full-length sequencing of 10X Genomics single-cell RNA-seq libraries is supported via the MAS-seq kit and protocol
  • Single-molecule resolution
  • Reads the same molecule multiple times for high consensus accuracy
  • Deeper biological insights, less data processing, and faster results thanks to the unmatched clarity of HiFi reads
  • Reliable and affordable high-throughput sequencing for a broad range of applications
  • Gain insight into difficult to sequence regions of the genome
  • Better data for superior results with lower coverage, from a single technology 

Coming Soon!

AVITI24

AVITI 24 with Teton CytoProfiling enables precise single cell spatial imaging of RNA, proteins, phospho-proteins, and cell morphology across hundreds of thousands to millions of cells. Use directly from cells (adherent or suspension) with no extraction or library prep required.