Sequencing

Illumina Sequencing Services

NovaSeq 6000

The Illumina NovaSeq 6000 is a high-end sequencer designed to provide some of the highest throughput in the market. It has the capacity to run up to two flow cells at a time, with 2 flow cell options to choose from. The highest throughput (S2) can process up to 3.3 billion reads and generate up to 1000 Gb of data (with 150 bp read lengths). The system is compatible with Illumina library preparation kits, and can support virtually any sequencing application- whole genome sequencing, whole transcriptomes, whole exomes, metagenomics, and more.

NextSeq 500

The Illumina NextSeq 500 System is a desktop sequencer with power and flexibility to carry out applications such as whole genome sequencing, exome sequencing, whole transcriptome sequencing, mRNA-Seq, and methylation sequencing, among others. In one run it can sequence up to 16 exomes, up to 20 transcriptomes, up to 96 targeted panels, up to 40 gene expression profiling samples, and a full human genome at 30x coverage. Users can choose between high output or mid output flow cell configurations. At high output, up to 800 million paired end reads can be generated (at 150 bp read length) to produce up to 120 Gb of data in 29 hours.

HiSeq 4000

The Illumina HiSeq 4000 sequencer is designed for processing more samples in greater depth to generate richer, more meaningful data to accomodate a wide range of ultra-high throughput sequencing applications. Its dual flow cell options feature a capacity of processing over 400 Gb per day or 1.5 Tb per run, with up to 5 billion single reads. In a single run it can sequence up to 12 whole human genomes (at 30x coverage), up to 96 exomes, and up to 100 transcriptomes, assuming 50 million reads per sample.

MiSeq

The Illumina MiSeq System offers capabilities for small whole-genome sequencing, amplicon sequencing, gene expression profiling, small RNA analysis and 16s metagenomic sequencing in a compact instruement. Using a high throughput kit, MiSeq can produce as much as 15 Gb of output from 50 million paired end reads (at a read length of 300 bp).

iSeq 100

The iSeq 100 Sequencing System makes next-generation sequencing easier and more affordable than ever. Designed for simplicity, it allows labs of all sizes to sequence DNA and RNA at the push of a button. The iSeq 100 System is ideal for small whole-genome sequencing (eg, bacteria, viruses, plasmids), targeted sequencing of a set of genes or gene regions, gene expression analysis, or 16S metagenomics.

Sequencer Specifications

	NovaSeq 6000 S4 (Per Lane)	NovaSeq 6000 S2 (Per Lane)	NextSeq 500	HiSeq 4000 (Per Lane)	MiSeq	iSeq 100
Maximum Reads Per Run (Paired End)	4 - 5 Billion	3.3 - 4.1 Billion	400 Million	350 Million	25 Million	4 Million
Maximum Output	1600 - 3000 Gb	667 - 1250 Gb	120 Gb	110 Gb	15 Gb	1.2 Gb
Run Time	36 - 44 hrs	25 - 36 hrs	12 - 30 hrs	<1 - 3.5 days	4 - 55 hrs	9 - 17.5 hrs
Maximum Read Length	2 x 150 bp	2 x 150 bp	2 x 150 bp	2 x 150 bp	2 x 300 bp	2 x 150 bp

Oxford Nanopore Sequencing Service

MinION

MinION is the only portable, real-time device for DNA and RNA sequencing, putting you in control of your sequence data. Each consumable flow cell can now generate as much as 30 Gb of DNA sequence data or 7-12 million reads if you are analysing RNA. Ultra-long read lengths are possible (hundreds of kb) as you can choose your fragment length. The MinION streams data in real time so that analysis can be performed during the experiment and workflows are fully versatile.

Sequencer Specification

Genapsys Sequencing Services

Genapsys Sequencer

GenapSys has developed a novel electrical-based platform capable of accurately detecting single base incorporations. The semiconductor chip-based detection modality allows the system to be compact, scalable, and affordable.Shifting from optics to electrical based detection creates a substantial reduction in capital cost. The simplicity of the detection modality that does not require any optics, robots, scanners, or expensive analysis servers, allows for a low cost and affordable instrument. The low per-run cost means reasonable cost per sample without excessive sample batching.