Library Construction

10X Genomics

Single Cell Gene Expression

Go beyond traditional gene expression analysis to characterize cell populations, cell types, cell states, and more on a cell-by-cell basis. From assessing tumor heterogeneity and stem cell composition, to dissecting neuronal populations—the technological advancements provided by the Chromium Single Cell Gene Expression Solution, along with turnkey software tools, allow the creation of high complexity libraries from single cells to maximize insight from any sample type.

Single Cell ATAC

The Chromium Single Cell ATAC (Assay for Transposase Accessible Chromatin) Solution accelerates the understanding of the regulatory landscape of the genome, thereby providing insights into cell variability. The chromatin profiling of tens of thousands of single cells in parallel allows researchers to see how chromatin compaction and DNA-binding proteins regulate gene expression at high resolution.

Single Cell Immune Profiling

The Chromium Single Cell Immune Profiling Solution is a comprehensive approach to simultaneously examine the cellular context of the adaptive immune response and immune repertoires of hundreds to tens of thousands of T and B cells in human or mouse on a cell-by-cell basis. With the addition of Feature Barcoding technology, you can also detect and analyze additional cellular readouts, such as cell surface markers and antigen specificity, to enhance immune cell phenotyping and study dynamic interactions between lymphocytes and target cells.

Spatial Gene Expression

The Visium Spatial Gene Expression Solution measures total mRNA in intact tissue sections and maps where gene activity is occurring. This solution provides a high-resolution view of gene expression variability localization as it applies to the study of cancer, neuroscience, developmental biology, and more. Preserving spatial context while identifying distinct groups of cells offers critical information about the relationship of cellular function, phenotype, and location in tissue microenvironments.

Single Cell CNV

The Chromium Single Cell CNV Solution provides a comprehensive, scalable solution for revealing genome heterogeneity and understanding clonal evolution. Study disease pathogenesis or characterize neuronal mosaicism at the single cell level.

Linked-Reads Genomics

The Chromium Genome Sequencing Solution enables you to fully reconstruct long range haplotypes, reveal structural variation, and detect variants in previously inaccessible and complex regions of the genome. By partitioning and barcoding high molecular weight (HMW) DNA to generate Linked-Reads, short-read information can be placed within the context of the whole genome, allowing you to gain a more comprehensive view of the genome than ever before. Reveal the true diploid nature of the human genome and unlock the full spectrum of variants such as small nucleotide variants (SNVs), indels, and large-scale structural rearrangements (including inversions and translocations) with the Chromium Genome Sequencing Solution.

We can support Hash-seq and Cite-seq worklflows!

What is Cell Hashing?

10X Compatible Cite-Seq Antibodies

RNA-Seq

KAPA Stranded mRNA-Seq

The KAPA Stranded mRNA-Seq Kit makes use of KAPA mRNA Capture Beads prior to library preparation to enrich for mRNA over non-polyadenylated species such as ribosomal, precursor or noncoding RNAs.

KAPA Stranded RNA-Seq with RiboErase

The KAPA Stranded RNA-Seq Kit with RiboErase (HMR) targets and depletes rRNA from various sample types (human, mouse, rat). Efficient rRNA depletion prior to library preparation results in increased coverage of transcripts of interest, including noncoding and precursor transcripts. This kit is suitable for total RNA input from human, mouse and rat species only.

TruSeq Stranded mRNA

Get precise measurement of mRNA strand orientation for detection of antisense transcription, enhanced transcript annotation, and increased alignment efficiency. High coverage uniformity enhances the discovery of features such as alternative transcripts, gene fusions, and allele-specific expression.

TruSeq Stranded Total RNA

Compatible with a wide range of samples, including low-quality DNA/RNA and formalin-fixed, paraffin-embedded (FFPE) samples, TruSeq Stranded Total RNA couples all of the benefits of TruSeq RNA technology with Ribo-Zero ribosomal RNA reduction chemistry. The product enables analysis of coding and multiple forms of noncoding RNA with precise measurement of strand orientation, uniform coverage, and high-confidence discovery of features such as alternative transcripts, gene fusions, and allele-specific expression.

We offer many other workflows such as SMARTer Stranded Total RNA-Seq kit v2 - Pico, Trio RNA-Seq, etc.

Other workflows are available upon request!

RNA-Seq Specifications

Kit	Input Amount	Compatible Sample Types	Compatible Species
KAPA Stranded mRNA-Seq	100 ng - 4 ug	High-Quality Total RNA	Eukaryotic (Animal, Plant, etc)
KAPA Stranded RNA-Seq with RiboErase	100 ng - 1 ug	High-Quality Total RNA, Degraded or FFPE Total RNA	Human, Mouse, and Rat
TruSeq Stranded mRNA-Seq	100 ng - 1 ug total RNA or 10 - 100 ng isolated mRNA	High Quality Total RNA or mRNA	Mammalian, Rat, Human, Bovine, Mouse
TruSeq Stranded Total RNA-Seq	100 ng - 1 ug	High Quality Total RNA, FFPE Tissue	Rat, Human, Mouse

DNA-Seq

Nextera XT

With Nextera technology, DNA is simultaneously fragmented and tagged with sequencing adapters in a single tube enzymatic reaction. Nextera XT supports ultra-low DNA input of only 1 ng. It enables a wide range of input samples, including small genomes, PCR amplicons greater than 300 bp, plasmids, microbial genomes, concatenated amplicons, and double-stranded cDNA.

Nextera DNA Flex

The Nextera DNA Flex workflow supports a broad DNA input range (1–500 ng), multiple sample types, and both small and large genomes. The workflow includes DNA extraction from blood, saliva, or dried blood spots or bacterial colonies. Gain the flexibility to sequence human or other large, complex genomes as well as amplicons or microbial species, all with a single kit.

SMARTer ThruPLEX DNA-Seq

The SMARTer ThruPLEX DNA-Seq Kit builds on innovative ThruPLEX chemistry to generate DNA libraries from as little as 50 pg of DNA, and improves sensitivity. This kit can be used with fragmented double-stranded DNA (dsDNA) from any sample source—genomic DNA, biofluids such as cell-free DNA (cfDNA), DNA from FFPE materials, and cDNA.

Methyl-Seq

NEXTFLEX Bisulfite Library Prep

The NEXTFLEX® Bisulfite-Seq Kit is a versatile kit designed to facilitate assessment of the methylation state of the genome and simplify workflow by using master mixed reagents and magnetic bead based cleanup to reduce pipetting and eliminate time-consuming steps in library preparation. This kit features Enhanced Adapter Ligation Technology, resulting in library preps with a larger number of unique sequencing reads. This specially designed NEXTFLEX® ligation enzymatic mix allows users to perform ligations with longer adapters and superior ligation efficiencies.

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