Welcome to the definitive website on protein microarray (PM) experiments*. (Note: The format is updated from Brian Kidd's original site (because Stanford updated the web hosting system). The original is available from the WayBack Machine from Archive.org). The site no longer exists.) In this website, Brian Kidd has detailed every step required to put your favorite peptides onto glass slides and get experimental results worthy of publication. Excluding this paragraph, all the content is written in his first person. The navigation bar on the left contains each step in chronological order; starting with Let's Get the Party Started (instructions for picking the peptide/protein list) and ending with Add a Figure to that Paper (instructions for consolidating all that hard work into a beautiful image that is fit for print). Without further ado, here's the protocol in Brian's words:
A successful PM experiment requires multiple steps. These steps build in a linear fashion and in chronological order. I thought a website would be the perfect media for communicating this information because it allows one to “click” through the steps and easily link to any part of the process. Each page contains detailed information for how to do each step and helpful suggestions for maximizing one’s success with this technique. Multiple cartoons augment the text where relevant. I’ve included images of screens that one is likely to encounter when using programs for data processing and analysis. In addition, this website contains links to internal and external resources that provide greater detail about particular aspects of the process. Finally, each page can be downloaded in either pdf or word format.
In creating this website, my primary goal was to provide a comprehensive tutorial for someone who has never done PM experiments. In writing this document, I assumed people would have no prior knowledge of microarray experiments or analysis. Therefore, I included enough detail that newcomers could use the PM technique to answer specific scientific questions. In addition to writing a tutorial, I also provided guidelines and reference material for people who have use the PM technique before and are familiar with carrying out these types of experiments. To that aim, I've included many checklists, explanations, and hints that I believe seasoned veterans will find helpful.
*The original instructions are archived in the Wayback Machine (www.archive.org).