Research Overview

We develop biotechnologies for genome engineering and synthetic biology to flexibly program and manipulate the genomic codes of life. To expand our bioengineering toolbox and to understand the function of genome in development and disease, we developed a suite of CRISPR tools, including the nuclease-deactivated dCas molecules from the natural CRISPR-Cas system for transcription activation or repression (CRISPRi/a), live-cell genome imaging (LiveFISH), and manipulating the spatial genome organization (CRISPR-GO). We appreciate the vast macromolecules (DNA, RNA, and proteins) that have been evolved from billion years history, and aim to harness these naturally occurring molecules as tools. We appreciate the power of directed evolution to obtain novel molecules that have been discovered or evolved in Nature. Topics are particularly interesting to us: