Bio

Education & Certifications


  • Bachelor of Arts, Cornell University, Biological Sciences (2003)

Stanford Advisors


Research & Scholarship

Current Research and Scholarly Interests


Functions of distal centriole proteins in cilia formation in monociliated and multiciliated cells
Applications of super-resolution imaging to cell biology

Lab Affiliations


Publications

Journal Articles


  • STED Microscopy with Optimized Labeling Density Reveals 9-Fold Arrangement of a Centriole Protein BIOPHYSICAL JOURNAL Lau, L., Lee, Y. L., Sahl, S. J., Stearns, T., Moerner, W. E. 2012; 102 (12): 2926-2935

    Abstract

    Super-resolution fluorescence microscopy can achieve resolution beyond the optical diffraction limit, partially closing the gap between conventional optical imaging and electron microscopy for elucidation of subcellular architecture. The centriole, a key component of the cellular control and division machinery, is 250 nm in diameter, a spatial scale where super-resolution methods such as stimulated emission depletion (STED) microscopy can provide previously unobtainable detail. We use STED with a resolution of 60 nm to demonstrate that the centriole distal appendage protein Cep164 localizes in nine clusters spaced around a ring of ?300 nm in diameter, and quantify the influence of the labeling density in STED immunofluorescence microscopy. We find that the labeling density dramatically influences the observed number, size, and brightness of labeled Cep164 clusters, and estimate the average number of secondary antibody labels per cluster. The arrangements are morphologically similar in centrioles of both proliferating cells and differentiated multiciliated cells, suggesting a relationship of this structure to function. Our STED measurements in single centrioles are consistent with results obtained by electron microscopy, which involve ensemble averaging or very different sample preparation conditions, suggesting that we have arrived at a direct measurement of a centriole protein by careful optimization of the labeling density.

    View details for DOI 10.1016/j.bpj.2012.05.015

    View details for Web of Science ID 000305546500027

    View details for PubMedID 22735543

  • STED Super-resolution Microscopy in Drosophila Tissue and in Mammalian Cells REPORTERS, MARKERS, DYES, NANOPARTICLES, AND MOLECULAR PROBES FOR BIOMEDICAL APPLICATIONS III Lau, L., Lee, Y. L., Matis, M., Axelrod, J., Stearns, T., Moerner, W. E. 2011; 7910

    View details for DOI 10.1117/12.881221

    View details for Web of Science ID 000297729300032

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