Bio

Professional Education


  • Bachelor of Science, Nanjing University (2012)
  • Doctor of Philosophy, University of Massachusetts Amherst (2018)

Stanford Advisors


Publications

All Publications


  • Multiplexed fluorescence in situ hybridization-based detection of circulating tumor cells: A novel liquid-based technology to facilitate accurate and early identification of non-small cell lung cancer patients. Cancer cytopathology Zhu, Y., Lowe, A. C. 2020

    View details for DOI 10.1002/cncy.22277

    View details for PubMedID 32320525

  • An in vitro Microscopy-based Assay for Microtubule-binding and Microtubule-crosslinking by Budding Yeast Microtubule-associated Protein BIO-PROTOCOL Zhu, Y., Tan, W., Lee, W. 2018; 8 (23)

    Abstract

    In this protocol, we describe a simple microscopy-based method to assess the interaction of a microtubule-associated protein (MAP) with microtubules. The interaction between MAP and microtubules is typically assessed by a co-sedimentation assay, which measures the amount of MAP that co-pellets with microtubules by centrifugation, followed by SDS-PAGE analysis of the supernatant and pellet fractions. However, MAPs that form large oligomers tend to pellet on their own during the centrifugation step, making it difficult to assess co-sedimentation. Here we describe a microscopy-based assay that measures microtubule binding by direct visualization using fluorescently-labeled MAP, solving the limitations of the co-sedimentation assay. Additionally, we recently reported quantification of microtubule bundling by measuring the thickness of individual microtubule structures observed in the microscopy-based assay, making the protocol more advantageous than the traditional microtubule co-pelleting assay.

    View details for DOI 10.21769/BioProtoc.3110

    View details for Web of Science ID 000458028500014

    View details for PubMedID 30733975

    View details for PubMedCentralID PMC6363367

  • Microtubule cross-linking activity of She1 ensures spindle stability for spindle positioning JOURNAL OF CELL BIOLOGY Zhu, Y., An, X., Tomaszewski, A., Hepler, P. K., Lee, W. 2017; 216 (9): 2759–75

    Abstract

    Dynein mediates spindle positioning in budding yeast by pulling on astral microtubules (MTs) from the cell cortex. The MT-associated protein She1 regulates dynein activity along astral MTs and directs spindle movements toward the bud cell. In addition to localizing to astral MTs, She1 also targets to the spindle, but its role on the spindle remains unknown. Using function-separating alleles, live-cell spindle assays, and in vitro biochemical analyses, we show that She1 is required for the maintenance of metaphase spindle stability. She1 binds and cross-links MTs via a C-terminal MT-binding site. She1 can also self-assemble into ring-shaped oligomers. In cells, She1 stabilizes interpolar MTs, preventing spindle deformations during movement, and we show that this activity is regulated by Ipl1/Aurora B phosphorylation during cell cycle progression. Our data reveal how She1 ensures spindle integrity during spindle movement across the bud neck and suggest a potential link between regulation of spindle integrity and dynein pathway activity.

    View details for DOI 10.1083/jcb.201701094

    View details for Web of Science ID 000409075500019

    View details for PubMedID 28794129

    View details for PubMedCentralID PMC5584168

  • The role of plus TIPs in directional tip expansion MOLECULAR MICROBIOLOGY Zhu, Y., Lee, W. 2014; 94 (3): 486–89

    Abstract

    Aspergillus nidulans is an ideal model to study nuclear migration and intracellular transport by dynein and kinesin owing to its long neuron-like hyphae, conserved transport mechanisms, and powerful genetics. In this organism, as in other filamentous fungi, microtubules have been implicated in patterning cell shape through polarized tip growth - the hallmark mode of growth that generates the elongated hyphae. Exactly how microtubules regulate tip growth is incompletely understood and remains a fascinating question for various cell types, such as pollen tubes and root hairs. Zeng et al. (2014) describe important new findings in A. nidulans regarding the role of EBA, the master regulator of microtubule plus end-tracking proteins, in specifying microtubule dynamics required for directional tip growth at the hyphal tip.

    View details for DOI 10.1111/mmi.12791

    View details for Web of Science ID 000344465000002

    View details for PubMedID 25213368

    View details for PubMedCentralID PMC4213288

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