Current Research and Scholarly Interests
Diabetic nephropathy contributes 44% cases of the chronic kidney disease. By comparing the transcriptional profile of diabetic mice with/without kidney disease, I discovered that several inflammatory regulatory genes determine the disease progression, which can be used as therapeutic targets and diagnostic biomarkers.
For the 1st time, I discovered a method to break kidney glomeruli into single cells and quantify them by flow cytometry, which eliminates the need of cell counting under microscope and speeds up the experiment from weeks to 2 days. This technology was used to analyze leukocyte infiltration in mice, and can be used in other fields of glomerular study.
With a successful injection rate of 80-90%, I perform hydrodynamic injection (tail vein) to over-express protein in mice for at least 12 weeks and study its biological function in type I diabetes and anti-GBM mice model with macroalbuminuria.
V5 peptide is commonly used to label recombinant proteins, yet its small size prevents the detection by two different antibodies of a sandwich ELISA. For the 1st time, I developed an anti-V5 competitive ELISA method which uses only one antibody to quantify V5 tagged recombinant protein in cell lines and mice. This ELISA can be used to confirm over-expression of recombinant protein in cell lines and mice serum by its V5 tag.