Bio

Honors & Awards


  • Cardiovascular Institute (CVI) Travel Award, Stanford School of Medicine (July 2013)
  • EMBO Short Term Fellowship, Institute for Stem Cell Therapy (I-STEM), France, European Molecular Biology Organization (EMBO) (May-June 2013)
  • Cardiovascular Institute (CVI) Seed Grant Award, Stanford University School of Medicine (December 2012)
  • Cardiovascular Institute (CVI) Young Investigator Award in Basic Sciences 2012, Stanford University School of Medicine (September 2012)
  • Oak Foundation Cardiovascular Award, Stanford School of Medicine (March 2011-2013)
  • McGill Engineering Doctoral Award (MEDA), McGill University (2007-2010)
  • Provost’s Graduate Fellowship, McGill University (2009)
  • Dr. Gerald Hatch Doctoral Fellowship Award, McGill University (2008)

Boards, Advisory Committees, Professional Organizations


  • Professional Member, American Heart Association (AHA) (2011 - Present)
  • Member, Canadian Biomaterials Society (CBS) (2008 - 2011)
  • Secretary/Treasurer Officer, Society for Biomaterials (2011 - 2013)
  • Member, Tissue Engineering and Regenerative Medicine International Society (TERMIS) (2011 - Present)

Professional Education


  • Doctor of Philosophy, McGill University (2010)
  • Masters, Sharif University of Technology, Materials Sci and Eng (2006)
  • Bachelor, Sharif University of Technology, Materials Sci and Tech (2003)

Stanford Advisors


Research & Scholarship

Current Research and Scholarly Interests


My research at Ruiz-Lozano's Lab is focused on the micromechanisms involved in cardiac remodeling/regeneration following severe heart injuries, particularly, post myocardial infarction (MI). By employing engineered 3D collagen scaffolds, we aim to develop cardiac patches associated with specific targeting characteristics in order to efficiently interfere with cardiac remodeling phenomena after MI. Various cell types, growth factors, functionalized nanoparticles, and specific-targeting peptides are seeded within the patches. Subsequently, the patch will be grafted onto the damaged heart tissue and cardiac function, remodeling, and regeneration are studied for up to 7 weeks post injury. Furthermore, by regulating the micromechanical properties of the 3D matrix (patch), we try to improve the patch-native tissue integration as well as the electromechanical coupling.

Lab Affiliations


Publications

Journal Articles


  • The effect of bioengineered acellular collagen patch on cardiac remodeling and ventricular function post myocardial infarction. Biomaterials Serpooshan, V., Zhao, M., Metzler, S. A., Wei, K., Shah, P. B., Wang, A., Mahmoudi, M., Malkovskiy, A. V., Rajadas, J., Butte, M. J., Bernstein, D., Ruiz-Lozano, P. 2013; 34 (36): 9048-9055

    Abstract

    Regeneration of the damaged myocardium is one of the most challenging fronts in the field of tissue engineering due to the limited capacity of adult heart tissue to heal and to the mechanical and structural constraints of the cardiac tissue. In this study we demonstrate that an engineered acellular scaffold comprising type I collagen, endowed with specific physiomechanical properties, improves cardiac function when used as a cardiac patch following myocardial infarction. Patches were grafted onto the infarcted myocardium in adult murine hearts immediately after ligation of left anterior descending artery and the physiological outcomes were monitored by echocardiography, and by hemodynamic and histological analyses four weeks post infarction. In comparison to infarcted hearts with no treatment, hearts bearing patches preserved contractility and significantly protected the cardiac tissue from injury at the anatomical and functional levels. This improvement was accompanied by attenuated left ventricular remodeling, diminished fibrosis, and formation of a network of interconnected blood vessels within the infarct. Histological and immunostaining confirmed integration of the patch with native cardiac cells including fibroblasts, smooth muscle cells, epicardial cells, and immature cardiomyocytes. In summary, an acellular biomaterial with specific biomechanical properties promotes the endogenous capacity of the infarcted myocardium to attenuate remodeling and improve heart function following myocardial infarction.

    View details for DOI 10.1016/j.biomaterials.2013.08.017

    View details for PubMedID 23992980

  • Exocytosis of nanoparticles from cells: Role in cellular retention and toxicity ADVANCES IN COLLOID AND INTERFACE SCIENCE Sakhtianchi, R., Minchin, R. F., Lee, K., Alkilany, A. M., Serpooshan, V., Mahmoudi, M. 2013; 201: 18-29
  • Temperature: The "Ignored" Factor at the NanoBio Interface ACS NANO Mahmoudi, M., Abdelmonem, A. M., Behzadi, S., Clement, J. H., Dutz, S., Ejtehadi, M. R., Hartmann, R., Kantner, K., Linne, U., Maffre, P., Metzler, S., Moghadam, M. K., Pfeiffer, C., Rezaei, M., Ruiz-Lozano, P., Serpooshan, V., Shokrgozar, M. A., Nienhaus, G. U., Parak, W. J. 2013; 7 (8): 6555-6562

    Abstract

    Upon incorporation of nanoparticles (NPs) into the body, they are exposed to biological fluids, and their interaction with the dissolved biomolecules leads to the formation of the so-called protein corona on the surface of the NPs. The composition of the corona plays a crucial role in the biological fate of the NPs. While the effects of various physico-chemical parameters on the composition of the corona have been explored in depth, the role of temperature upon its formation has received much less attention. In this work, we have probed the effect of temperature on the protein composition on the surface of a set of NPs with various surface chemistries and electric charges. Our results indicate that the degree of protein coverage and the composition of the adsorbed proteins on the NPs surface depend on the temperature at which the protein corona is formed. Also, the uptake of NPs is affected by the temperature. Temperature is, thus, an important parameter that needs to be carefully controlled in quantitative studies of bio-nano interactions.

    View details for DOI 10.1021/nn305337c

    View details for Web of Science ID 000323810600013

    View details for PubMedID 23808533

  • Effect of chitosan incorporation on the consolidation process of highly-hydrated collagen hydrogel scaffolds Soft Matter Chicatun, F., Muja, N., Serpooshan, V., Quinn, T. M., Nazhat, S. N. 2013

    View details for DOI 10.1039/C3SM52176A

  • Plasma concentration gradient influences the protein corona decoration on nanoparticles RSC ADVANCES Ghavami, M., Saffar, S., Abd Emamy, B., Peirovi, A., Shokrgozar, M. A., Serpooshan, V., Mahmoudi, M. 2013; 3 (4): 1119-1126

    View details for DOI 10.1039/c2ra22093h

    View details for Web of Science ID 000312390000023

  • Hydraulic permeability of multilayered collagen gel scaffolds under plastic compression-induced unidirectional fluid flow ACTA BIOMATERIALIA Serpooshan, V., Quinn, T. M., Muja, N., Nazhat, S. N. 2013; 9 (1): 4673-4680

    Abstract

    Under conditions of free fluid flow, highly hydrated fibrillar collagen gels expel fluid and undergo gravity driven consolidation (self-compression; SC). This process can be accelerated by the application of a compressive stress (plastic compression; PC) in order to generate dense collagen scaffolds for tissue engineering. To define the microstructural evolution of collagen gels under PC, this study applied a two-layer micromechanical model that was previously developed to measure hydraulic permeability (k) under SC. Radially confined PC resulted in unidirectional fluid flow through the gel and the formation of a dense lamella at the fluid expulsion boundary which was confirmed by confocal microscopy of collagen immunoreactivity. Gel mass loss due to PC and subsequent SC were measured and applied to Darcy's law to calculate the thickness of the lamella and hydrated layer, as well as their relative permeabilities. Increasing PC level resulted in a significant increase in mass loss fraction and lamellar thickness, while the thickness of the hydrated layer dramatically decreased. Permeability of lamella also decreased from 1.8×10(-15) to 1.0×10(-15) m(2) in response to an increase in PC level. Ongoing SC, following PC, resulted in a uniform decrease in mass loss and k with increasing PC level and as a function SC time. Experimental k data were in close agreement with those estimated by the Happel model. Calculation of average k values for various two-layer microstructures indicated that they each approached 10(-15)-10(-14) m(2) at equilibrium. In summary, the two-layer micromechanical model can be used to define the microstructure and permeability of multi-layered biomimetic scaffolds generated by PC.

    View details for DOI 10.1016/j.actbio.2012.08.031

    View details for Web of Science ID 000313376900022

    View details for PubMedID 22947324

  • Antibacterial properties of nanoparticles TRENDS IN BIOTECHNOLOGY Hajipour, M. J., Fromm, K. M., Ashkarran, A. A., Jimenez de Aberasturi, D., Ruiz de Larramendi, I., Rojo, T., Serpooshan, V., Parak, W. J., Mahmoudi, M. 2012; 30 (10): 499-511

    Abstract

    Antibacterial agents are very important in the textile industry, water disinfection, medicine, and food packaging. Organic compounds used for disinfection have some disadvantages, including toxicity to the human body, therefore, the interest in inorganic disinfectants such as metal oxide nanoparticles (NPs) is increasing. This review focuses on the properties and applications of inorganic nanostructured materials and their surface modifications, with good antimicrobial activity. Such improved antibacterial agents locally destroy bacteria, without being toxic to the surrounding tissue. We also provide an overview of opportunities and risks of using NPs as antibacterial agents. In particular, we discuss the role of different NP materials.

    View details for DOI 10.1016/j.tibtech.2012.06.004

    View details for Web of Science ID 000309946600002

    View details for PubMedID 22884769

  • Silver-Coated Engineered Magnetic Nanoparticles Are Promising for the Success in the Fight against Antibacterial Resistance Threat ACS NANO Mahmoudi, M., Serpooshan, V. 2012; 6 (3): 2656-2664

    Abstract

    The combination of patients with poor immune system, prolonged exposure to anti-infective drugs, and cross-infection has given rise to nosocomial infections with highly resistant pathogens, which is going to be a growing threat so termed "antibiotic resistance". Due to their significant antimicrobial activity, silver nanoparticles are recognized as a promising candidate to fight against resistant pathogens; however, there are two major shortcomings with these nanoparticles. First, the silver nanoparticles are highly toxic to the healthy cells; second, due to the protection offered by the biofilm mode of growth, the silver nanoparticles cannot eradicate bacterial biofilms. In order to overcome these limitations, this study introduces a new class of engineered multimodal nanoparticles comprising a magnetic core and a silver ring with a ligand gap. The results indicated promising capability of the designed multimodal nanoparticles for high-yield antibacterial effects and eradication of bacterial biofilms, while the particles were completely compatible with the cells. Utilizing a gold ring as an intermediate coating on the produced nanoparticles may exploit new opportunities for theranosis applications. This will require special consideration in future works.

    View details for DOI 10.1021/nn300042m

    View details for Web of Science ID 000301945900083

    View details for PubMedID 22397679

  • Large Protein Absorptions from Small Changes on the Surface of Nanoparticles JOURNAL OF PHYSICAL CHEMISTRY C Mahmoudi, M., Serpooshan, V. 2011; 115 (37): 18275-18283

    View details for DOI 10.1021/jp2056255

    View details for Web of Science ID 000294875200032

  • Fibroblast contractility and growth in plastic compressed collagen gel scaffolds with microstructures correlated with hydraulic permeability. Journal of biomedical materials research. Part A Serpooshan, V., Muja, N., Marelli, B., Nazhat, S. N. 2011; 96 (4): 609-620

    Abstract

    Scaffold microstructure is hypothesized to influence physical and mechanical properties of collagen gels, as well as cell function within the matrix. Plastic compression under increasing load was conducted to produce scaffolds with increasing collagen fibrillar densities ranging from 0.3 to above 4.1 wt % with corresponding hydraulic permeability (k) values that ranged from 1.05 to 0.03 ?m², as determined using the Happel model. Scanning electron microscopy revealed that increasing the level of collagen gel compression yielded a concomitant reduction in pore size distribution and a slight increase in average fibril bundle diameter. Decreasing k delayed the onset of contraction and significantly reduced both the total extent and the maximum rate of contraction induced by NIH3T3 fibroblasts seeded at a density of either 6.0 x 10? or 1.5 x 10? cells mL?¹. At the higher cell density, however, the effect of k reduction on collagen gel contraction was overcome by an accelerated onset of contraction which led to an increase in both the total extent and the maximum rate of contraction. AlamarBlue? measurements indicated that the metabolic activity of fibroblasts within collagen gels increased as k decreased. Moreover, increasing seeded cell density from 2.0 x 10? to 1.5 x 10? cells mL?¹ significantly increased NIH3T3 proliferation. In conclusion, fibroblast-matrix interactions can be optimized by defining the microstructural properties of collagen scaffolds through k adjustment which in turn, is dependent on the cell seeding density.

    View details for DOI 10.1002/jbm.a.33008

    View details for PubMedID 21268235

  • Rodent model for adult stem cell transplantation for bone repair Journal of Bone and Joint Surgery Gao, C., Nguyen, O., Serpooshan, V., El Chaarani, B., Nazhat, S. N., Harvey, E. J., Henderson, J. E. 2011; 93: 553
  • Large Protein Absorptions from Small Changes on the Surface of Nanoparticles Journal of Physical Chemistry Morteza Mahmoudi, Vahid Serpooshan 2011
  • Characterization and modelling of a dense lamella formed during self-compression of fibrillar collagen gels: implications for biomimetic scaffolds Soft Matter Serpooshan, Thomas M. Quinn, Naser Muja, Showan N. Nazhat 2011; 7: 2918
  • Engineered nanoparticles for biomolecular imaging NANOSCALE Mahmoudi, M., Serpooshan, V., Laurent, S. 2011; 3 (8): 3007-3026

    Abstract

    In recent years, the production of nanoparticles (NPs) and exploration of their unusual properties have attracted the attention of physicists, chemists, biologists and engineers. Interest in NPs arises from the fact that the mechanical, chemical, electrical, optical, magnetic, electro-optical and magneto-optical properties of these particles are different from their bulk properties and depend on the particle size. There are numerous areas where nanoparticulate systems are of scientific and technological interest, particularly in biomedicine where the emergence of NPs with specific properties (e.g. magnetic and fluorescence) for contrast agents can lead to advancing the understanding of biological processes at the biomolecular level. This review will cover a full description of the physics of various imaging methods, including MRI, optical techniques, X-rays and CT. In addition, the effect of NPs on the improvement of the mentioned non-invasive imaging methods will be discussed together with their advantages and disadvantages. A detailed discussion will also be provided on the recent advances in imaging agents, such as fluorescent dye-doped silica NPs, quantum dots, gold- and engineered polymeric-NPs, superparamagnetic iron oxide NPs (SPIONs), and multimodal NPs (i.e. nanomaterials that are active in both MRI and optical methods), which are employed to overcome many of the limitations of conventional contrast agents (e.g. gadolinium).

    View details for DOI 10.1039/c1nr10326a

    View details for Web of Science ID 000293521700001

    View details for PubMedID 21717012

  • Reduced hydraulic permeability of three-dimensional collagen scaffolds attenuates gel contraction and promotes the growth and differentiation of mesenchymal stem cells ACTA BIOMATERIALIA Serpooshan, V., Julien, M., Nguyen, O., Wang, H., Li, A., Muja, N., Henderson, J. E., Nazhat, S. N. 2010; 6 (10): 3978-3987

    Abstract

    Optimal scaffold characteristics are essential for the therapeutic application of engineered tissues. Hydraulic permeability (k) affects many properties of collagen gels, such as mechanical properties, cell-scaffold interactions within three dimensions (3D), oxygen flow and nutrient diffusion. However, the cellular response to 3D gel scaffolds of defined k values has not been investigated. In this study, unconfined plastic compression under increasing load was used to produce collagen gels with increasing solid volume fractions. The Happel model was used to calculate the resulting permeability values in order to study the interaction of k with gel mechanical properties and mesenchymal stem cell (MSC)-induced gel contraction, metabolism and differentiation in both non-osteogenic (basal medium) and osteogenic medium for up to 3 weeks. Collagen gels of fibrillar densities ranging from 0.3 to >4.1 wt.% gave corresponding k values that ranged from 1.00 to 0.03 microm(2). Mechanical testing under compression showed that the collagen scaffold modulus increased with collagen fibrillar density and a decrease in k value. MSC-induced gel contraction decreased as a direct function of decreasing k value. Relative to osteogenic conditions, non-osteogenic MSC cultures exhibited a more than 2-fold increase in gel contraction. MSC metabolic activity increased similarly under both osteogenic and non-osteogenic culture conditions for all levels of plastic compression. Under osteogenic conditions MSC differentiation and mineralization, as indicated by alkaline phosphatase activity and von Kossa staining, respectively, increased in response to an elevation in collagen fibrillar density and decreased gel permeability. In this study, gel scaffolds with higher collagen fibrillar densities and corresponding lower k values provided a greater potential for MSC differentiation and appear most promising for bone grafting purposes. Thus, cell-scaffold interactions can be optimized by defining the 3D properties of collagen scaffolds through k adjustment.

    View details for DOI 10.1016/j.actbio.2010.04.028

    View details for Web of Science ID 000282207100017

    View details for PubMedID 20451675

  • Effect of rubber particle cavitation on the mechanical properties and deformation behavior of high-impact polystyrene Journal of Applied Polymer Science Vahid Serpooshan, S. Zokaei, R. Bagheri 2007; 104 (2): 1110

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