Bio

Professional Education


  • Doctor of Philosophy, Jawharlal Nehru Centre for Advanced Sci Research (2016)
  • Master of Technology, Vellore Institute Technology (2010)
  • Bachelor of Technology, Anna University (2007)

Publications

All Publications


  • Toll-Like Receptor 9 Activation Rescues Impaired Antibody Response in Needle-free Intradermal DNA Vaccination SCIENTIFIC REPORTS Arunachalam, P. S., Mishra, R., Badarinath, K., Selvam, D., Payeli, S. K., Stout, R. R., Ranga, U. 2016; 6

    Abstract

    The delivery of plasmid DNA to the skin can target distinct subsets of dermal dendritic cells to confer a superior immune response. The needle-free immunization technology offers a reliable, safe and efficient means to administer intradermal (ID) injections. We report here that the ID injection of DNA vectors using an NF device (NF-ID) elicits a superior cell-mediated immune response, at much lesser DNA dosage, comparable in magnitude to the traditional intramuscular immunization. However, the humoral response is significantly impaired, possibly at the stage of B cell isotype switching. We found that the NF-ID administration deposits the DNA primarily on the epidermis resulting in a rapid loss of the DNA as well as the synthesized antigen due to the faster regeneration rate of the skin layers. Therefore, despite the immune-rich nature of the skin, the NF-ID immunization of DNA vectors may be limited by the impaired humoral response. Additional booster injections are required to augment the antibody response. As an alternative and a viable solution, we rescued the IgG response by coadministration of a Toll-like receptor 9 agonist, among other adjuvants examined. Our work has important implication for the optimization of the emerging needle-free technology for ID immunization.

    View details for DOI 10.1038/srep33564

    View details for Web of Science ID 000383946000001

    View details for PubMedID 27658623

    View details for PubMedCentralID PMC5034244

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