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  • Not in the Same Vein: Inflammatory Bowel Disease, Malignancy, and Enterocolic Lymphocytic Phlebitis. Digestive diseases and sciences Farber, O. N., Weingarden, A., Lee, C., Braxton, D. R., Bingham, D., Scott, G., Fernandez-Becker, N., Goff, D., Shelton, A., Kin, C. 2020

    View details for DOI 10.1007/s10620-020-06425-w

    View details for PubMedID 32594463

  • Author Spotlight: Orly N. Farber. Digestive diseases and sciences Farber, O. N. 2020

    View details for DOI 10.1007/s10620-020-06426-9

    View details for PubMedID 32583221

  • Clonally expanded ?? T cells protect against Staphylococcus aureus skin reinfection. The Journal of clinical investigation Dillen, C. A., Pinsker, B. L., Marusina, A. I., Merleev, A. A., Farber, O. N., Liu, H., Archer, N. K., Lee, D. B., Wang, Y., Ortines, R. V., Lee, S. K., Marchitto, M. C., Cai, S. S., Ashbaugh, A. G., May, L. S., Holland, S. M., Freeman, A. F., Miller, L. G., Yeaman, M. R., Simon, S. I., Milner, J. D., Maverakis, E., Miller, L. S. 2018; 128 (3): 1026?42

    Abstract

    The mechanisms that mediate durable protection against Staphylococcus aureus skin reinfections are unclear, as recurrences are common despite high antibody titers and memory T cells. Here, we developed a mouse model of S. aureus skin reinfection to investigate protective memory responses. In contrast with WT mice, IL-1?-deficient mice exhibited poor neutrophil recruitment and bacterial clearance during primary infection that was rescued during secondary S. aureus challenge. The ?? T cells from skin-draining LNs utilized compensatory T cell-intrinsic TLR2/MyD88 signaling to mediate rescue by trafficking and producing TNF and IFN-?, which restored neutrophil recruitment and promoted bacterial clearance. RNA-sequencing (RNA-seq) of the LNs revealed a clonotypic S. aureus-induced ?? T cell expansion with a complementarity-determining region 3 (CDR3) aa sequence identical to that of invariant V?5+ dendritic epidermal T cells. However, this T cell receptor ? (TRG) aa sequence of the dominant CDR3 sequence was generated from multiple gene rearrangements of TRGV5 and TRGV6, indicating clonotypic expansion. TNF- and IFN-?-producing ?? T cells were also expanded in peripheral blood of IRAK4-deficient humans no longer predisposed to S. aureus skin infections. Thus, clonally expanded ?? T cells represent a mechanism for long-lasting immunity against recurrent S. aureus skin infections.

    View details for DOI 10.1172/JCI96481

    View details for PubMedID 29400698

    View details for PubMedCentralID PMC5824877

  • Diminished allergic disease in patients with STAT3 mutations reveals a role for STAT3 signaling in mast cell degranulation. The Journal of allergy and clinical immunology Siegel, A. M., Stone, K. D., Cruse, G., Lawrence, M. G., Olivera, A., Jung, M. Y., Barber, J. S., Freeman, A. F., Holland, S. M., O'Brien, M., Jones, N., Nelson, C. G., Wisch, L. B., Kong, H. H., Desai, A., Farber, O., Gilfillan, A. M., Rivera, J., Milner, J. D. 2013; 132 (6): 1388?96

    Abstract

    Severe atopic conditions associated with elevated serum IgE are heterogeneous with few known causes. Nearly every patient with autosomal-dominant hyper-IgE syndrome (AD-HIES) due to signal transducer and activator of transcription 3 (STAT3) mutations has a history of eczematous dermatitis and elevated IgE; however, clinical atopy has never been systematically studied.Understanding of genetic determinants of allergic disease may lead to novel therapies in controlling allergic disease.We conducted clinical evaluation of the rates of food allergies and anaphylaxis in patients with AD-HIES, a cohort of patients with no STAT3 mutation but with similar histories of elevated IgE and atopic dermatitis, and healthy volunteers with no history of atopy. Morphine skin prick testing, ImmunoCAP assays for allergen-specific IgE, and basophil activation were measured. A model of systemic anaphylaxis was studied in transgenic mice carrying an AD-HIES mutation. STAT3 was silenced in LAD2 and primary human mast cells to study the role of STAT3 in signaling and degranulation after IgE cross-linking.Food allergies and anaphylaxis were markedly diminished in patients with AD-HIES compared with a cohort of patients with no STAT3 mutation but with similar histories of elevated IgE and atopic dermatitis. Morphine skin prick testing and basophil activation were diminished in patients with AD-HIES, whereas mice carrying an AD-HIES mutation were hyporesponsive to systemic anaphylaxis models. Rapid mast cell STAT3 serine727 phosphorylation was noted after IgE cross-linking, and inhibition of STAT3 signaling in mast cells lead to impaired Fc?RI-mediated proximal and distal signaling, as well as reduced degranulation.This study serves as an example for how mutations in specific atopic pathways can lead to discrete allergic phenotypes, encompassing increased risk of some phenotypes but a relative protection from others.

    View details for DOI 10.1016/j.jaci.2013.08.045

    View details for PubMedID 24184145

    View details for PubMedCentralID PMC3881191

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