zzzs

Bio

Bio

I am currently working at Stanford University School of Medicine as a Life Science Research Professional. As a Research Associate, I worked at Translational Bioscience on projects aiding the discovery and development of precision medicine. I received a Master of Engineering degree at Illinois Institute of Technology at Chicago, Illinois in the United States with the department of Biomedical Engineering in May 2017. Additionally, I have completed a 5 year Integrated Bachelor's of Sciences and Master's of Sciences degree program at the Indian Institute of Science, Education and Research, Bhopal majoring in Biological Sciences with minor in Chemistry. My primary research interests lie in molecular and cellular biology, immunology, and cancer biology. Moreover, I have worked on short projects in Core Java programming and in relational database management systems while pursuing my Diploma.

I am actively interested in research and development as well as explore public health administration, healthcare management and policy making.

Current Role at Stanford

1. Perform complex (lab and/or field) experiments for a major portion of the research project.
Maintain detailed records of experiments and outcomes
2. Interpret and analyze results, often where few guidelines exist. Based on prior results,
contribute to development of new research protocols and procedures or adaptation of existing
protocols
3. Make recommendations on plan design and/or research direction to principal investigator
4. Work with health and safety department to ensure lab complies with regulations and all
required trainings
5. Participate in multidisciplinary teams across different faculties or schools
6. Oversee supply and equipment budget under supervision of principal investigator or business
manager
7. Perform ongoing literature review to remain current with new procedures and related research;
present findings for lab work group
8. Contribute substantively to the preparation of papers for publication, especially in the results
section.
9. May contribute to publication of findings and present ongoing work and findings to colleagues
at academic conferences
10. Supervise (either formally or informally) staff or students as needed, including oversight and
instruction on techniques, as well as consultation on project work. Serve as technical resource
for other research staff
11. Perform general lab maintenance as needed; maintain lab stock, manage chemical inventory
and safety records, and provide general lab support as needed. May assist with purchasing
laboratory equipment

Contact

Publications

All Publications

  • HuR Displaces Polypyrimidine Tract Binding Protein To Facilitate La Binding to the 3 ' Untranslated Region and Enhances Hepatitis C Virus Replication JOURNAL OF VIROLOGY Shwetha, S., Kumar, A., Mullick, R., Vasudevan, D., Mukherjee, N., Das, S. 2015; 89 (22): 11356-11371

    Abstract

    HuR is a ubiquitous, RNA binding protein that influences the stability and translation of several cellular mRNAs. Here, we report a novel role for HuR, as a regulator of proteins assembling at the 3' untranslated region (UTR) of viral RNA in the context of hepatitis C virus (HCV) infection. HuR relocalizes from the nucleus to the cytoplasm upon HCV infection, interacts with the viral polymerase (NS5B), and gets redistributed into compartments of viral RNA synthesis. Depletion in HuR levels leads to a significant reduction in viral RNA synthesis. We further demonstrate that the interaction of HuR with the 3' UTR of the viral RNA affects the interaction of two host proteins, La and polypyrimidine tract binding protein (PTB), at this site. HuR interacts with La and facilitates La binding to the 3' UTR, enhancing La-mediated circularization of the HCV genome and thus viral replication. In addition, it competes with PTB for association with the 3' UTR, which might stimulate viral replication. Results suggest that HuR influences the formation of a cellular/viral ribonucleoprotein complex, which is important for efficient initiation of viral RNA replication. Our study unravels a novel strategy of regulation of HCV replication through an interplay of host and viral proteins, orchestrated by HuR.Hepatitis C virus (HCV) is highly dependent on various host factors for efficient replication of the viral RNA. Here, we have shown how a host factor (HuR) migrates from the nucleus to the cytoplasm and gets recruited in the protein complex assembling at the 3' untranslated region (UTR) of HCV RNA. At the 3' UTR, it facilitates circularization of the viral genome through interaction with another host factor, La, which is critical for replication. Also, it competes with the host protein PTB, which is a negative regulator of viral replication. Results demonstrate a unique strategy of regulation of HCV replication by a host protein through alteration of its subcellular localization and interacting partners. The study has advanced our knowledge of the molecular mechanism of HCV replication and unraveled the complex interplay between the host factors and viral RNA that could be targeted for therapeutic interventions.

    View details for DOI 10.1128/JVI.01714-15

    View details for Web of Science ID 000363467200017

    View details for PubMedID 26339049

    View details for PubMedCentralID PMC4645635

Footer Links:

Stanford Medicine Resources: