Bio

Honors & Awards


  • T. C. Hsu Memorial Research Scholarship, MD Anderson Cancer Center UT Health Graduate School (2016)
  • GSBS Endowment in Biochemistry and Molecular Biology Research Award, MD Anderson Cancer Center UT Health Graduate School (2015)
  • CPRIT - Graduate Scholar, MD Anderson Cancer Center UT Health Graduate School (2015-2016)
  • Center for Cancer Epigenetics Scholar, MD Anderson Cancer Center UT Health Graduate School (2013-2014)

Professional Education


  • Doctor of Philosophy, Univ Texas Health Science Ctr-Houston (2016)
  • M.S., University of Pune, Microbiology (2008)

Stanford Advisors


Publications

All Publications


  • Generation of Stable Expression Mammalian Cell Lines Using Lentivirus. Bio-protocol Tandon, N., Thakkar, K. N., LaGory, E. L., Liu, Y., Giaccia, A. J. 2018; 8 (21)

    Abstract

    Lentiviruses are used very widely to generate stable expression mammalian cell lines. They are used for both gene down-regulation (by using shRNA) or for gene up-regulation (by using ORF of gene of interest). The technique of generating stable cell lines using 3rd generation lentivirus is very robust and it typically takes about 1-2 weeks to get stable expression for most mammalian cell lines. The advantage of using the 3rd generation lentivirus are that are very safe and they are replication incompetent.

    View details for PubMedID 30505888

  • Cross-talk between chromatin acetylation and SUMOylation of tripartite motif-containing protein 24 (TRIM24) impacts cell adhesion. The Journal of biological chemistry Appikonda, S., Thakkar, K. N., Shah, P. K., Dent, S. Y., Andersen, J. N., Barton, M. C. 2018

    Abstract

    Proteins with domains that recognize and bind post-translational modifications (PTMs) of histones are collectively termed epigenetic readers. Numerous interactions between specific reader protein domains and histone PTMs and their regulatory outcomes have been reported, but little is known about how reader proteins may in turn be modulated by these interactions. Tripartite motif-containing protein 24 (TRIM24) is a histone reader aberrantly expressed in multiple cancers. Here, our investigation revealed functional crosstalk between histone acetylation and TRIM24 SUMOylation. Binding of TRIM24 to chromatin via its tandem PHD-bromodomain, which recognizes unmethylated lysine 4 and acetylated lysine 23 of histone H3 (H3K4me0/K23ac), led to TRIM24 SUMOylation at lysine residues 723 and 741. Inactivation of the bromodomain, either by mutation or with a small-molecule inhibitor, IACS-9571, abolished TRIM24 SUMOylation. Conversely, inhibition of histone deacetylation markedly increased TRIM24's interaction with chromatin and its SUMOylation. Of note, gene expression profiling of MCF7 cells expressing wild type versus SUMO-deficient TRIM24 identified cell adhesion as the major pathway regulated by the cross-talk between chromatin acetylation and TRIM24 SUMOylation. In conclusion, our findings establish a new link between histone H3 acetylation and SUMOylation of the reader protein TRIM24, a functional connection that may bear on TRIM24's oncogenic function and may inform future studies of PTM cross-talk between histones and epigenetic regulators.

    View details for PubMedID 29523690

  • Regulation of gene expression in human cancers by TRIM24 Drug Discovery Today: Technologies Appikonda, S., Thakkar, K., Barton, M. 2016
  • Tissue-specific metabolism and TRIM24. Aging Thakkar, K. N., Stratton, S. A., Craig Barton, M. 2015; 7 (10): 736-737

    View details for PubMedID 26454661

  • TRIM24 links glucose metabolism with transformation of human mammary epithelial cells ONCOGENE Pathiraja, T. N., Thakkar, K. N., Jiang, S., Stratton, S., Liu, Z., Gagea, M., Shi, X., Shah, P. K., Phan, L., Lee, M., Andersen, J., Stampfer, M., Barton, M. C. 2015; 34 (22): 2836-2845

    Abstract

    Tripartite motif 24 protein (TRIM24) is a plant homeodomain/bromodomain histone reader, recently associated with poor overall survival of breast-cancer patients. At a molecular level, TRIM24 is a negative regulator of p53 levels and a co-activator of estrogen receptor. However, the role of TRIM24 in breast tumorigenesis remains largely unknown. We used an isogenic human mammary epithelial cell (HMEC) culture model, derived from reduction mammoplasty tissue, and found that ectopic expression of TRIM24 in immortalized HMECs (TRIM24 iHMECs) greatly increased cellular proliferation and induced malignant transformation. Subcutaneous injection of TRIM24 iHMECs in nude mice led to growth of intermediate to high-grade tumors in 60-70% of mice. Molecular analysis of TRIM24 iHMECs revealed a glycolytic and tricarboxylic acid cycle gene signature, alongside increased glucose uptake and activated aerobic glycolysis. Collectively, these results identify a role for TRIM24 in breast tumorigenesis through reprogramming of glucose metabolism in HMECs, further supporting TRIM24 as a viable therapeutic target in breast cancer.

    View details for DOI 10.1038/onc.2014.220

    View details for Web of Science ID 000355324300003

    View details for PubMedID 25065590

  • Biological synthesis of metallic nanoparticles NANOMEDICINE-NANOTECHNOLOGY BIOLOGY AND MEDICINE Thakkar, K. N., Mhatre, S. S., Parikh, R. Y. 2010; 6 (2): 257-262

    Abstract

    The synthesis of metallic nanoparticles is an active area of academic and, more importantly, "application research" in nanotechnology. A variety of chemical and physical procedures could be used for synthesis of metallic nanoparticles. However, these methods are fraught with many problems including use of toxic solvents, generation of hazardous by-products, and high energy consumption. Accordingly, there is an essential need to develop environmentally benign procedures for synthesis of metallic nanoparticles. A promising approach to achieve this objective is to exploit the array of biological resources in nature. Indeed, over the past several years, plants, algae, fungi, bacteria, and viruses have been used for production of low-cost, energy-efficient, and nontoxic metallic nanoparticles. In this review, we provide an overview of various reports of synthesis of metallic nanoparticles by biological means.This review provides an overview of various methods of synthesis of metallic nanoparticles by biological means. Many chemical and physical procedures used for synthesis of metallic nanoparticles are fraught with major problems: toxic solvents, hazardous by-products, high energy consumption. Over the past several years, plants, algae, fungi, bacteria, and viruses have been used for production of low-cost, energy-efficient, and nontoxic metallic nanoparticles.

    View details for DOI 10.1016/j.nano.2009.07.002

    View details for Web of Science ID 000276090600007

    View details for PubMedID 19616126

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