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  • Functional characterization of BRCC3 mutations in acute myeloid leukemia with t(8;21)(q22;q22.1) LEUKEMIA Meyer, T., Jahn, N., Lindner, S., Roehner, L., Dolnik, A., Weber, D., Scheffold, A., Koepff, S., Paschka, P., Gaidzik, V. I., Heckl, D., Wiese, S., Ebert, B. L., Doehner, H., Bullinger, L., Doehner, K., Kroenke, J. 2020; 34 (2): 404?15

    Abstract

    BRCA1/BRCA2-containing complex 3 (BRCC3) is a Lysine 63-specific deubiquitinating enzyme (DUB) involved in inflammasome activity, interferon signaling, and DNA damage repair. Recurrent mutations in BRCC3 have been reported in myelodysplastic syndromes (MDS) but not in de novo AML. In one of our recent studies, we found BRCC3 mutations selectively in 9/191 (4.7%) cases with t(8;21)(q22;q22.1) AML but not in 160 cases of inv(16)(p13.1q22) AML. Clinically, AML patients with BRCC3 mutations had an excellent outcome with an event-free survival of 100%. Inactivation of BRCC3 by CRISPR/Cas9 resulted in improved proliferation in t(8;21)(q22;q22.1) positive AML cell lines and together with expression of AML1-ETO induced unlimited self-renewal in mouse hematopoietic progenitor cells in vitro. Mutations in BRCC3 abrogated its deubiquitinating activity on IFNAR1 resulting in an impaired interferon response and led to diminished inflammasome activity. In addition, BRCC3 inactivation increased release of several cytokines including G-CSF which enhanced proliferation of AML cell lines with t(8;21)(q22;q22.1). Cell lines and primary mouse cells with inactivation of BRCC3 had a higher sensitivity to doxorubicin due to an impaired DNA damage response providing a possible explanation for the favorable outcome of BRCC3 mutated AML patients.

    View details for DOI 10.1038/s41375-019-0578-6

    View details for Web of Science ID 000523481800009

    View details for PubMedID 31576005

    View details for PubMedCentralID PMC7214237

  • Impact of NPM1/FLT3-ITD genotypes defined by the2017 European LeukemiaNet in patients with acute myeloid leukemia. Blood Döhner, K., Thiede, C., Jahn, N., Panina, E., Gambietz, A., Larson, R. A., Prior, T. W., Marcucci, G., Jones, D., Krauter, J., Heuser, M., Voso, M. T., Ottone, T., Nomdedeu, J. F., Mandrekar, S. J., Klisovic, R., Wei, A. H., Sierra, J., Sanz, M. A., Brandwein, J., de Witte, T. M., Jansen, J. H., Niederwieser, D., Appelbaum, F., Medeiros, B. C., Tallman, M. S., Schlenk, R. F., Ganser, A., Serve, H., Ehninger, G., Amadori, S., Gathmann, I., Benner, A., Pallaud, C., Stone, R. M., Döhner, H., Bloomfield, C. D. 2019

    Abstract

    Patients with AML harboring FLT3 internal tandem duplications (ITD) have poor outcomes, in particular AML with a high ({greater than or equal to}0.5) mutant-to-wildtype allelic ratio (AR). The 2017 European LeukemiaNet (ELN) recommendations defined four distinct FLT3-ITD genotypes based on the ITD-AR and the NPM1 mutational status. In this retrospective, exploratory study, we investigated the prognostic and predictive impact of the NPM1/FLT3-ITD genotypes categorized according to the 2017 ELN risk groups in patients randomized within the RATIFY trial evaluating the addition of midostaurin to standard chemotherapy. The four NPM1/FLT3-ITD genotypes significantly differed with regard to clinical and concurrent genetic features. Complete ELN risk categorization could be done in 318 of 549 trial patients with FLT3-ITD AML. Significant factors for response after one or two induction cycles were ELN risk group and white blood cell (WBC) counts; treatment with midostaurin had no influence. Overall survival (OS) differed significantly between ELN risk groups with estimated 5-year OS probabilities of 0.63, 0.43, and 0.33 for favorable-, intermediate- and adverse-risk groups, respectively (P<0.001). Multivariate Cox model for OS using allogeneic hematopoietic-cell transplantation (HCT) in first complete remission as a time-dependent variable revealed treatment with midostaurin, allogeneic HCT, ELN favorable-risk group, and lower WBC counts as significant favorable factors. In this model, there was a consistent beneficial effect of midostaurin across ELN risk groups.

    View details for DOI 10.1182/blood.2019002697

    View details for PubMedID 31826241

  • Incidence and prognostic impact of ASXL2 mutations in adult acute myeloid leukemia patients with t(8;21)(q22;q22): a study of the German-Austrian AML Study Group LEUKEMIA Jahn, N., Agrawal, M., Bullinger, L., Weber, D., Corbacioglu, A., Gaidzik, V. I., Schmalbrock, L., Thol, F., Heuser, M., Krauter, J., Goehring, G., Kuendgen, A., Fiedler, W., Wattad, M., Held, G., Koehne, C., Horst, H., Luebbert, M., Ganser, A., Schlenk, R. F., Doehner, H., Doehner, K., Paschka, P. 2017; 31 (4): 1012?15

    View details for DOI 10.1038/leu.2017.18

    View details for Web of Science ID 000398261800033

    View details for PubMedID 28090090

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