Bio

Professional Education


  • Doctor of Philosophy, University of Alberta (2009)

Stanford Advisors


Publications

Journal Articles


  • Synthesis of modified Trichinella spiralis disaccharide epitopes and a comparison of their recognition by chemical mapping and saturation transfer difference NMR CARBOHYDRATE RESEARCH Cui, L., Ling, C., Sadowska, J., Bundle, D. R. 2014; 383: 1-13

    Abstract

    A rat monoclonal antibody 9D4 raised against the cell surface N-glycan of the parasite Trichinella spirallis protects rats against further infection. The terminal disaccharide β-d-Tyvp(1→3)β-d-GalNAcp (2) represents the immunodominant portion of the antigenic determinant. Chemical mapping of the antibody binding site by functional group modification employing monodeoxy and mono-O-methyl congeners identified key polar contacts and topography of the bound disaccharide. We report here a comparison of the chemical mapping studies with the antigen topography inferred from saturation transfer difference (STD) NMR experiments. During chemical mapping several congeners of compound 2 showed substantially enhanced binding. Pairing of these functional group modifications to create derivatives 6 and 7 did not show additive free energy gains and STD NMR data point to small variations in mode of binding as a probable cause. Improved syntheses of disaccharides 2-7 are reported.

    View details for DOI 10.1016/j.carres.2013.10.012

    View details for Web of Science ID 000328745400001

    View details for PubMedID 24239604

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