Bio

Professional Education


  • Bachelor of Science, University of Wisconsin Madison (2007)
  • Doctor of Philosophy, Harvard University (2012)

Stanford Advisors


Publications

Journal Articles


  • Anterograde or retrograde transsynaptic labeling of CNS neurons with vesicular stomatitis virus vectors PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA Beier, K. T., Saunders, A., Oldenburg, I. A., Miyamichi, K., Akhtar, N., Luo, L., Whelan, S. P., Sabatini, B., Cepko, C. L. 2011; 108 (37): 15414-15419

    Abstract

    To understand how the nervous system processes information, a map of the connections among neurons would be of great benefit. Here we describe the use of vesicular stomatitis virus (VSV) for tracing neuronal connections in vivo. We made VSV vectors that used glycoprotein (G) genes from several other viruses. The G protein from lymphocytic choriomeningitis virus endowed VSV with the ability to spread transsynaptically, specifically in an anterograde direction, whereas the rabies virus glycoprotein gave a specifically retrograde transsynaptic pattern. The use of an avian G protein fusion allowed specific targeting of cells expressing an avian receptor, which allowed a demonstration of monosynaptic anterograde tracing from defined cells. Synaptic connectivity of pairs of virally labeled cells was demonstrated by using slice cultures and electrophysiology. In vivo infections of several areas in the mouse brain led to the predicted patterns of spread for anterograde or retrograde tracers.

    View details for DOI 10.1073/pnas.1110854108

    View details for Web of Science ID 000294804900082

    View details for PubMedID 21825165

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