Current Research and Scholarly Interests
The Elias Lab seeks to develop and apply methods for large scale proteome characterization to solve fundamental problems in cell biology and disease. The growing field of proteomics has the lofty goal of characterizing the proteins in any isolated complex, subcellular compartment, cultured cell line or tissue. We use mass spectrometry-based approaches which can do more than simply determining the identity of a protein isolated in a polyacrylamide gel -- rapidly advancing technologies are allowing us to measure dynamic changes in protein abundances, post-translational modification states, splice isoforms, interaction partners, and localization across multiple cell states. The combination of liquid chromatography with tandem mass spectrometry (LC-MS/MS) has emerged as the most robust technology for making proteome-scale discoveries. Although the tools of proteomics have greatly advanced in recent years, many challenges lie ahead. Our lab focuses on developing new methods in protein fractionation, instrumentation, and data analysis to meet these challenges, and then applies them to studying important biomedical paradigms, including antigen presentation in cancer, and monitoring host responses to the gut microbiome..