Bio

Honors & Awards


  • NSF Graduate Research Fellowship (GRFP) Recipient, NSF (2019)

Education & Certifications


  • Bachelor of Arts, UC Berkeley, Molecular and Cell Biology: Immunology & Infectious Diseases, Gender and Women's Studies (2017)

Stanford Advisors


Professional

Work Experience


  • Research Associate, Infectious Disease Initiative, Chan Zuckerberg Biohub (June 5, 2017 - August 16, 2019)

    Location

    San Francisco, CA

Publications

All Publications


  • Clinical features, diagnostics, and outcomes of patients presenting with acute respiratory illness: A retrospective cohort study of patients with and without COVID-19. EClinicalMedicine Shah, S. J., Barish, P. N., Prasad, P. A., Kistler, A., Neff, N., Kamm, J., Li, L. M., Chiu, C. Y., Babik, J. M., Fang, M. C., Kangelaris, K. N., Langelier, C., Abe-Jones, Y., Alipanah, N., Alvarez, F. N., Botvinnik, O. B., Castaneda, G., Dadasovich, R. M., Davis, J., Deng, X., DeRisi, J. L., Detweiler, A. M., Federman, S., Haliburton, J., Hao, S., Kerkhoff, A. D., Kumar, G. R., Malcolm, K. B., Mann, S. A., Martinez, S., Mary, R. K., Mick, E., Mwakibete, L., Najafi, N., Peluso, M. J., Phelps, M., Pisco, A. O., Ratnasiri, K., Rubio, L. A., Sellas, A., Sherwood, K. D., Sheu, J., Spottiswoode, N., Tan, M., Yu, G. 2020: 100518

    Abstract

    Most data on the clinical presentation, diagnostics, and outcomes of patients with COVID-19 have been presented as case series without comparison to patients with other acute respiratory illnesses.We examined emergency department patients between February 3 and March 31, 2020 with an acute respiratory illness who were tested for SARS-CoV-2. We determined COVID-19 status by PCR and metagenomic next generation sequencing (mNGS). We compared clinical presentation, diagnostics, treatment, and outcomes.Among 316 patients, 33 tested positive for SARS-CoV-2; 31 without COVID-19 tested positive for another respiratory virus. Among patients with additional viral testing (27/33), no SARS-CoV-2 co-infections were identified. Compared to those who tested negative, patients with COVID-19 reported longer symptoms duration (median 7d vs. 3d, p < 0.001). Patients with COVID-19 were more often hospitalized (79% vs. 56%, p = 0.014). When hospitalized, patients with COVID-19 had longer hospitalizations (median 10.7d vs. 4.7d, p < 0.001) and more often developed ARDS (23% vs. 3%, p < 0.001). Most comorbidities, medications, symptoms, vital signs, laboratories, treatments, and outcomes did not differ by COVID-19 status.While we found differences in clinical features of COVID-19 compared to other acute respiratory illnesses, there was significant overlap in presentation and comorbidities. Patients with COVID-19 were more likely to be admitted to the hospital, have longer hospitalizations and develop ARDS, and were unlikely to have co-existent viral infections.National Center for Advancing Translational Sciences, National Heart Lung Blood Institute, National Institute of Allergy and Infectious Diseases, Chan Zuckerberg Biohub, Chan Zuckerberg Initiative.

    View details for DOI 10.1016/j.eclinm.2020.100518

    View details for PubMedID 32864588

    View details for PubMedCentralID PMC7447618

  • Flavivirus NS1 Triggers Tissue-Specific Vascular Endothelial Dysfunction Reflecting Disease Tropism CELL REPORTS Puerta-Guardo, H., Glasner, D. R., Espinosa, D. A., Biering, S. B., Patana, M., Ratnasiri, K., Wang, C., Beatty, P., Harris, E. 2019; 26 (6): 1598-+

    Abstract

    Flaviviruses cause systemic or neurotropic-encephalitic pathology in humans. The flavivirus nonstructural protein 1 (NS1) is a secreted glycoprotein involved in viral replication, immune evasion, and vascular leakage during dengue virus infection. However, the contribution of secreted NS1 from related flaviviruses to viral pathogenesis remains unknown. Here, we demonstrate that NS1 from dengue, Zika, West Nile, Japanese encephalitis, and yellow fever viruses selectively binds to and alters permeability of human endothelial cells from lung, dermis, umbilical vein, brain, and liver in vitro and causes tissue-specific vascular leakage in mice, reflecting the pathophysiology of each flavivirus. Mechanistically, each flavivirus NS1 leads to differential disruption of endothelial glycocalyx components, resulting in endothelial hyperpermeability. Our findings reveal the capacity of a secreted viral protein to modulate endothelial barrier function in a tissue-specific manner both in vitro and in vivo, potentially influencing virus dissemination and pathogenesis and providing targets for antiviral therapies and vaccine development.

    View details for DOI 10.1016/j.celrep.2019.01.036

    View details for Web of Science ID 000457709200019

    View details for PubMedID 30726741

  • Dengue virus NS1 cytokine-independent vascular leak is dependent on endothelial glycocalyx components PLOS PATHOGENS Glasner, D. R., Ratnasiri, K., Puerta-Guardo, H., Espinosa, D. A., Beatty, P., Harris, E. 2017; 13 (11): e1006673

    Abstract

    Dengue virus (DENV) is the most prevalent, medically important mosquito-borne virus. Disease ranges from uncomplicated dengue to life-threatening disease, characterized by endothelial dysfunction and vascular leakage. Previously, we demonstrated that DENV nonstructural protein 1 (NS1) induces endothelial hyperpermeability in a systemic mouse model and human pulmonary endothelial cells, where NS1 disrupts the endothelial glycocalyx-like layer. NS1 also triggers release of inflammatory cytokines from PBMCs via TLR4. Here, we examined the relative contributions of inflammatory mediators and endothelial cell-intrinsic pathways. In vivo, we demonstrated that DENV NS1 but not the closely-related West Nile virus NS1 triggers localized vascular leak in the dorsal dermis of wild-type C57BL/6 mice. In vitro, we showed that human dermal endothelial cells exposed to DENV NS1 do not produce inflammatory cytokines (TNF-?, IL-6, IL-8) and that blocking these cytokines does not affect DENV NS1-induced endothelial hyperpermeability. Further, we demonstrated that DENV NS1 induces vascular leak in TLR4- or TNF-? receptor-deficient mice at similar levels to wild-type animals. Finally, we blocked DENV NS1-induced vascular leak in vivo using inhibitors targeting molecules involved in glycocalyx disruption. Taken together, these data indicate that DENV NS1-induced endothelial cell-intrinsic vascular leak is independent of inflammatory cytokines but dependent on endothelial glycocalyx components.

    View details for DOI 10.1371/journal.ppat.1006673

    View details for Web of Science ID 000416888500010

    View details for PubMedID 29121099

    View details for PubMedCentralID PMC5679539

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