Bio

Current Role at Stanford


Oversee day to day operations in rodent and aquatic animal health programs including clinical medicine, health surveillance, import / export affairs, and strategic planning spreading across over a dozen facilities on and off campus. Supervise personnel engaged in rodent health surveillance enterprise. Administer appropriate veterinary care to all animals in AAALAC accredited Stanford research colonies and make critical health care decisions in a wide variety of situations. Perform veterinary care physical exams, diagnostic work-ups, medical or surgical treatment, and/or euthanasia, for all species found in the research colony populations. Ensure accuracy of clinical records in compliance with state and federal regulations. Provide veterinary care in the areas of preoperative, intraoperative and postoperative care of research animals. Participate in clinical and didactic training of residents (ACLAM sanctioned), externs, and visiting veterinary students. Provide assessments of animals prior to intrastate, interstate, and international shipments, including physical examination, review of colony history, and pertinent diagnostic tests. Review animal use proposals for the IACUC and coordinate the monitoring of approved research projects. Supervise and training for Animal Care Assistants, Veterinary Technicians, or lower-level Veterinarians as appropriate.

Service, Volunteer and Community Work


  • JoVE Reviewer, Journal of Video Experimentation (JoVE) (July 1, 2013 - Present)

    Review details on proper and humane usage of laboratory animals.

    Location

    USA

Professional

Professional Affiliations and Activities


  • Newsletter Editor, Northern California Branch AALAS (2017 - Present)
  • President, Northern California Branch AALAS (2016 - 2017)
  • Vice President, Northern California Branch AALAS (2015 - 2016)
  • Workshop Chair, Northern California Branch AALAS (2009 - 2012)

Publications

All Publications


  • Longevity in a fish bowl LAB ANIMAL Chu, D. 2016; 45 (3): 93-93

    View details for Web of Science ID 000371197200014

    View details for PubMedID 26886667

  • A "Pedi" Cures All: Toenail Trimming and the Treatment of Ulcerative Dermatitis in Mice PLOS ONE Adams, S. C., Garner, J. P., Felt, S. A., Geronimo, J. T., Chu, D. K. 2016; 11 (1)
  • Three cheers for the three-spined stickleback Heng, K., Thompson, A., Chu, D., Kingsley, D. M. Nature Publishing. 2016 ; Lab Animal (volumn 45 number 11): 421
  • Processing and secretion of ROP13: A unique Toxoplasma effector protein INTERNATIONAL JOURNAL FOR PARASITOLOGY Turetzky, J. M., Chu, D. K., Hajagos, B. E., Bradley, P. J. 2010; 40 (9): 1037-1044

    Abstract

    Like most intracellular pathogens, Toxoplasma synthesizes and secretes an arsenal of proteins to successfully invade its host cell and hijack host functions for intracellular survival. The rhoptries are key secretory organelles that inject proteins into the host cell where they are positioned to co-opt host processes, although little is known regarding how these proteins exert their functions. We show here that the rhoptry protein ROP13 is synthesized as a pre-pro-protein that is processed in the parasite. Processing occurs at a conserved SphiXE cleavage site as mutagenesis of glutamic acid to alanine at the P1 position disrupts ROP13 maturation. We also demonstrate that processing of the prodomain is not necessary for rhoptry targeting and secretion. While gene disruption reveals that ROP13 is not essential for growth in fibroblasts in vitro or for virulence in vivo, we find that ROP13 is a soluble effector protein that can access the cytoplasm of host cells. Exogenously expressed ROP13 in human cells remains cytosolic but also appears toxic, suggesting that over-expression of this effector protein is disrupting some function within the host cell.

    View details for DOI 10.1016/j.ijpara.2010.02.014

    View details for Web of Science ID 000280022700006

    View details for PubMedID 20359481

  • Alternative Group Housing for Small Bullfrog Colonies Chu, D. American Association for Laboratory Animal Science. Memphis, TN. 2010 ; Tech Talk (15(3)): 3
  • Uterine epithelioid trophoblastic tumor in an African green monkey (Chlorocebus aethiops sabaeus) 56th National Meeting of the American-Association-for-Laboratory-Animal-Science (AALAS) Chu, D., Shih, I., Knezevich, M., Sheth, S. AMER ASSOC LABORATORY ANIMAL SCIENCE. 2007: 92?96

    Abstract

    A uterine mass was detected on physical exam in a multiparous African green monkey as an incidental finding, and the well-circumscribed mass was removed via hysterectomy. Histologically, the mass consisted of sheets, nests, and cords of uniform intermediate trophoblastic cells with eosinophilic or clear cytoplasm. These neoplastic cells aggregated around blood vessels, forming islands of viable tumor cells amid extensive areas of coagulative necrosis with calcification in a 'geographic' pattern of necrosis. Immunohistochemistry of the trophoblastic cells revealed strong and diffuse staining for pancytokeratin AE1/3 and p63, with weak and moderate staining for human placental lactogen and placental alkaline phosphatase, respectively. Immunohistochemical staining for smooth muscle actin, epithelial membrane antigen, and human chorionic gonadotropin was negative. Overall, the histologic and immunohistochemical features of this tumor were consistent with those of epithelioid trophoblastic tumor. This rare tumor type has not been reported previously to occur in African green monkeys.

    View details for Web of Science ID 000245325200015

    View details for PubMedID 17343360

  • Comparing isoflurane with tribromoethanol anesthesia for echocardiographic phenotyping of transgenic mice JOURNAL OF THE AMERICAN ASSOCIATION FOR LABORATORY ANIMAL SCIENCE Chu, D. K., Jordan, M. C., Kim, J. K., Couto, M. A., Roos, K. P. 2006; 45 (4): 8-13

    Abstract

    Cardiac phenotyping of transgenic mice typically requires anesthesia. Chemical-grade tribromoethanol (TBE) is commonly used for this purpose due to its relatively short duration of action, modest cardiodepressive effects, and its noncontrolled status. In the present study, we used both genders of C57BL/6;C3H-Tg(Slc8a1)hKdp transgenic (TG) mice and C57BL/6;C3H wild-type (WT) mice to evaluate isoflurane (ISF) as a pharmaceutical-grade alternative to TBE for echocardiography and electrocardiography. Baseline target physiologic heart rates (beats per minute) were established by use of telemetry as 544 +/- 10 in WT mice and 580 +/- 21 in TG mice. TG and WT animals were anesthetized with either 0.8% to 1% inhalational ISF or 250 mg/kg intraperitoneal TBE. The following parameters were measured or calculated according to the previously defined physiologic heart rates: end diastolic and systolic dimensions; posterior wall and ventricular septal thicknesses; left ventricular mass, aortic ejection times; left ventricular fractional shortening; velocity of circumferential fiber shortening; and left ventricular ejection fraction. No significant difference between anesthetics was found for any measured cardiac parameters. However, the time required for data acquisition was significantly shorter for ISF (10 min) than for TBE (14 min). This study demonstrates that comparable echocardiographic results can be obtained at higher throughput by use of pharmaceuticalgrade ISF than with chemical-grade TBE.

    View details for Web of Science ID 000239352300002

    View details for PubMedID 16884172

  • Arthropod infestation in a colony of mice LAB ANIMAL Chu, D. K., Couto, M. A. 2005; 34 (9): 25-27

    View details for Web of Science ID 000232348700005

    View details for PubMedID 16195733

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