B.Sc., University of Iceland, Biochemistry (2004)
Doctor of Philosophy, University Of Dublin, Trinity College (2008)
AC Matin, Postdoctoral Faculty Sponsor
My main research projects are focused on the investigation of the therapeutic effect of a novel prodrug-enzyme anticancer therapy and the development of a magnetic resonance imaging system that would enable the assessment of tumor metastasis in vivo, starting from a single cell. I have gained valuable experience in molecular cloning, protein manufacture and protein purification. My previous research included the pre-clinical testing of various different fluorescent reporters, anticancer agents and photoresponsive antibacterial therapeutics. I have successfully collaborated with a range of other researcher, leading to five peer-reviewed publications and and I believe that my diverse research background will be of major help to the proposed project.
Herein we describe a new antimicrobial photodynamic therapeutic (PDT) agent based upon the brominated BF(2) chelated tetraarylazadipyrromethene photosensitizer class. Bis-ammonium salt substitution of the photosensitizer promoted a rapid 10 min uptake into Gram-positive and -negative bacterial strains and pathogenic yeasts. A photosensitizer and light dose response analysis for methicillin-sensitive S. aureus showed an impressive antibacterial efficacy with 1, 2, and 5 ?g/mL 6. Specifically, light activation with a dose of 16 J/cm(2) and 5 ?g/mL 6 resulted in a 6.8 and 3.4 log(10) reduction of S. aureus and a clinically defined methicillin-resistant Staphylococcus aureus (MRSA) strain, respectively. Encouragingly, a broad spectrum pathogen response (using 5 ?g/mL 6 and 75 J/cm(2)) was observed with 3.6 and 5.7 log(10) decreases in viable cell numbers achievable for Gram-negative bacterium E. coli and the pathogenic yeast C. albicans, respectively. The photophysical and cell eradicating characteristics of this bis-cationic PDT agent suggest that it has broad potential in antimicrobial therapeutics.
View details for DOI 10.1021/jm100585j
View details for Web of Science ID 000283106300007
View details for PubMedID 20879783
Strategic incorporation of sulfonic acid, carboxylic acid or ammonium salt motifs generate water soluble BF(2)-chelated tetraarylazadipyrromethenes which exhibit strong near infra-red (NIR) emissions above 720 nm and can be readily imaged in both eukaryotic and prokaryotic cells.
View details for DOI 10.1039/b919546g
View details for Web of Science ID 000273745100003
View details for PubMedID 20090964
The synthesis and photophysical characteristics of a pH responsive near-infrared fluorescence imaging probe is described. A key feature is the ability to conjugate the probe by an alkyne-azide cycloaddition reaction and its reversible response of fluorescence intensity across the physiological pH range.
View details for DOI 10.1021/ol902140v
View details for Web of Science ID 000272038200007
View details for PubMedID 19883098
The synthesis and photophysical and biological investigation of fluorescent 1,8-naphthalimide conjugated Troger's bases 1-3 are described. These structures bind strongly to DNA in competitive media at pH 7.4, with concomitant modulation in their fluorescence emission. These structures also undergo rapid cellular uptake, being localized within the nucleus within a few hours, and are cytotoxic against HL60 and (chronic myeloid leukemia) K562 cell lines.
View details for DOI 10.1021/ol9013602
View details for Web of Science ID 000269670700004
View details for PubMedID 19681640
The facile synthesis and photophysical characterization of new on-bead fluorophores and fluorescent sensors are described. The unique covalent immobilization strategy results in highly fluorescent beads with sharp emission profiles between 650 and 800 nm. Illustrative examples include imaging in an aqueous cellular environment and adaptation to include off/on sensing functionality, proven by a prototypical detection of gaseous HCl.
View details for DOI 10.1021/ol901413u
View details for Web of Science ID 000268796700030
View details for PubMedID 19624121