Bio

Professional Education


  • Doctor of Philosophy, University of Illinois Chicago (2010)

Stanford Advisors


Publications

Journal Articles


  • Generation and characterization of transgene-free human induced pluripotent stem cells and conversion to putative clinical-grade status STEM CELL RESEARCH & THERAPY Awe, J. P., Lee, P. C., Ramathal, C., Vega-Crespo, A., Durruthy-Durruthy, J., Cooper, A., Karumbayaram, S., Lowry, W. E., Clark, A. T., Zack, J. A., Sebastiano, V., Kohn, D. B., Pyle, A. D., Martin, M. G., Lipshutz, G. S., Phelps, P. E., Pera, R. A., Byrne, J. A. 2013; 4

    View details for DOI 10.1186/scrt246

    View details for Web of Science ID 000323720300001

  • Divergent RNA-Binding Proteins, DAZL and VASA, Induce Meiotic Progression in Human Germ Cells Derived In Vitro STEM CELLS Medrano, J. V., Ramathal, C., Nguyen, H. N., Simon, C., Pera, R. A. 2012; 30 (3): 441-451

    Abstract

    Our understanding of human germ cell development is limited in large part due to inaccessibility of early human development to molecular genetic analysis. Pluripotent human embryonic stem cells (hESCs) and induced pluripotent stem cells (iPSCs) have been shown to differentiate to cells of all three embryonic germ layers, as well as germ cells in vitro, and thus may provide a model for the study of the genetics and epigenetics of human germline. Here, we examined whether intrinsic germ cell translational, rather than transcriptional, factors might drive germline formation and/or differentiation from human pluripotent stem cells in vitro. We observed that, with overexpression of VASA (DDX4) and/or DAZL (Deleted in Azoospermia Like), both hESCs and iPSCs differentiated to primordial germ cells, and maturation and progression through meiosis was enhanced. These results demonstrate that evolutionarily unrelated and divergent RNA-binding proteins can promote meiotic progression of human-derived germ cells in vitro. These studies describe an in vitro model for exploring specifics of human meiosis, a process that is remarkably susceptible to errors that lead to different infertility-related diseases.

    View details for DOI 10.1002/stem.1012

    View details for Web of Science ID 000300611400012

    View details for PubMedID 22162380

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