Cheng Huang

Journal Articles

• Requirement of the combination of mushroom body ? lobe and ?/? lobes for the retrieval of both aversive and appetitive early memories in Drosophila Learning & Memory Xie, Z., Huang, C., Ci, B., Wang, L., Zhong, Y. 2013; 20 (9): 474-481

  View details for DOI 10.1101/lm.031823.113

• Dissociation of rugose-dependent short-term memory component from memory consolidation in Drosophila. Genes, brain, and behavior Zhao, J., Lu, Y., Zhao, X., Yao, X., Shuai, Y., Huang, C., Wang, L., Jeong, S. H., Zhong, Y. 2013

  Abstract

  Extensive investigations show several molecular and neuroanatomical mechanisms underlying short-lived and long-lasting memory in Drosophila. At the molecular level, the genetic pathway of memory formation, which was obtained through mutant research, seems to occur sequentially. So far, studies of Drosophila mutants appear to support the idea that mutants defective in short-term memory (STM) are always associated with long-term memory (LTM) impairment. At the neuroanatomical level, distinct memory traces are partially independently distributed. However, whether memory phase dissociation also exists at the molecular level remains unclear. Here, we report on molecular separation of STM and consolidated memory through genetic dissection of rugose mutants. Mutants in the rugose gene, which encodes an evolutionarily conserved A-kinase anchor protein, show immediate memory defects as assayed through aversive olfactory conditioning. Intriguingly, two well-defined consolidated memory components, anesthesia-resistant memory and protein synthesis-dependent LTM, are both normal in spite of the defective immediate memory after 10-session massed and spaced training. Moreover, rugose genetically interacts with cyclic AMP-protein kinase A signaling during STM formation. Considering our previous study that AKAP Yu specifically participates in LTM formation, these results suggest that there exists a molecular level of memory phase dissociation with distinct AKAPs in Drosophila.

  View details for PubMedID 23790035

• The differential requirement of mushroom body ?/? subdivisions in long-term memory retrieval in Drosophila. Protein & cell Huang, C., Wang, P., Xie, Z., Wang, L., Zhong, Y. 2013

  Abstract

  The mushroom body (MB), a bilateral brain structure possessing about 2000-2500 neurons per hemisphere, plays a central role in olfactory learning and memory in Drosophila melanogaster. Extensive studies have demonstrated that three major types of MB neurons (?/?, ?'/?' and ?) exhibit distinct functions in memory processing, including the critical role of approximately 1000 MB ?/? neurons in retrieving long-term memory. Inspired by recent findings that MB ?/? neurons can be further divided into three subdivisions (surface, posterior and core) and wherein the ?/? core neurons play an permissive role in long-term memory consolidation, we examined the functional differences of all the three morphological subdivisions of MB ?/? by temporally precise manipulation of their synaptic outputs during long-term memory retrieval. We found the normal neurotransmission from a combination of MB ?/? surface and posterior neurons is necessary for retrieving both aversive and appetitive long-term memory, whereas output from MB ?/? posterior or core subdivision alone is dispensable. These results imply a specific requirement of about 500 MB ?/? neurons in supporting long-term memory retrieval and a further functional partitioning for memory processing within the MB ?/? region.

  View details for PubMedID 23722532

• A Permissive Role of Mushroom Body alpha/beta Core Neurons in Long-Term Memory Consolidation in Drosophila CURRENT BIOLOGY Huang, C., Zheng, X., Zhao, H., Li, M., Wang, P., Xie, Z., Wang, L., Zhong, Y. 2012; 22 (21): 1981-1989

  Abstract

  Memories are not created equally strong or persistent for different experiences. In Drosophila, induction of long-term memory (LTM) for aversive olfactory conditioning requires ten spaced repetitive training trials, whereas a single trial is sufficient for LTM generation in appetitive olfactory conditioning. Although, with the ease of genetic manipulation, many genes and brain structures have been related to LTM formation, it is still an important task to identify new components and reveal the mechanisms underlying LTM regulation.Here we show that single-trial induction of LTM can also be achieved for aversive olfactory conditioning through inhibition of highwire (hiw)-encoded E3 ubiquitin ligase activity or activation of its targeted proteins in a cluster of neurons, localized within the ?/? core region of the mushroom body. Moreover, the synaptic output of these neurons is critical within a limited posttraining interval for permitting consolidation of both aversive and appetitive LTM.We propose that these ?/? core neurons serve as a "gate" to keep LTM from being formed, whereas any experience capable of "opening" the gate is given permit to be consolidated into LTM.

  View details for DOI 10.1016/j.cub.2012.08.048

  View details for Web of Science ID 000311060200018

  View details for PubMedID 23063437

• Involvement of reactive oxygen species and calcium in photo-induced membrane damage in HeLa cells by a bis-methanophosphonate fullerene JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY Qiao, X., Huang, C., Ying, Y., Yang, X., Liu, Y., Tian, Q. 2010; 98 (3): 193-198

  Abstract

  Photo-excited bioactivities of fullerene derivatives are attracting much attention. In this report, a bis-methanophosphonate fullerene (BMPF) and the other two fullerene derivatives, a bis-malonic acid fullerene (BMAF) and a fullerol were incubated with HeLa cells and irradiated with a green light emitted from a mercury lamp on a fluorescent microscopy. By using DNA fluorescent probe propidium iodide staining method, damage towards cell membrane could be detected when cells were treated by irradiation altogether with BMPF or BMAF at a low concentration (4 microM), and the damage was dose-dependent. The activity of BMPF was much higher than that of BMAF, while fullerol had no effects under the same condition. It was also revealed that different kinds of reactive oxygen species (ROS) correlated to BMPF and BMAF. Additionally, presence of extracellular calcium could promote the activities of both derivatives, while removal of extracellular calcium could not abort their membrane-damaged activities. These results indicated that ROS and calcium were involved in the photosensitization of fullerene derivatives, and BMPF was a superior photosensitizer which would find potential application in biomedical field.

  View details for DOI 10.1016/j.jphotobiol.2010.01.001

  View details for Web of Science ID 000276116600004

  View details for PubMedID 20144875

• Inhibition of DNA restrictive endonucleases by aqueous nanoparticle suspension of methanophosphonate fullerene derivatives and its mechanisms Science in China Series B: Chemistry Song, G., Lu, Y., Huang, C., Xie, X., Tan, X., Yang, X. 2009; 52 (5): 626-631

  View details for DOI 10.1007/s11426-009-0030-2

• Photo-induced lipid peroxidation of erythrocyte membranes by a bis-methanophosphonate fullerene TOXICOLOGY IN VITRO Yang, X. L., Huang, C., Qiao, X. G., Yao, L., Zhao, D. X., Tan, X. 2007; 21 (8): 1493-1498

  Abstract

  Using human erythrocyte membranes (EMs) as a model system, we have examined photo-induced lipid peroxidation by a bis-methanophosphonate fullerene (BMPF) and four other fullerene derivatives including a mono-methanophosphonic acid fullerene (MMPF), a dimalonic acid C(60) (DMA C(60)), a trimalonic acid C(60) (TMA C(60)) and a polyhydroxylated fullerene (fullerol). Lipid peroxidation was assessed as the malondialdehyde (MDA) level measured by the thiobarbituric acid assay. It was observed that BMPF increased the MDA level of EMs after irradiation in both time- and dose-dependent manners. The photo-induced activity became very significant (p<0.01) under the conditions of either the concentration of 10 microM and irradiation time of 30 min or the concentration of 5 microM and irradiation time of 60 min. Involvement of reactive oxygen species (ROS) in the activity was also examined by specific inhibitors of singlet oxygen, superoxide anions and hydroxyl radicals, respectively. While all three kinds were found responsible for the activity, the former two might play more important roles than the last one. Furthermore, the activity of BMPF was the strongest among all tested fullerene derivatives. These results indicated BMPF was a potential photosensitizer that would find application in photodynamic therapy.

  View details for DOI 10.1016/j.tiv.2007.06.008

  View details for Web of Science ID 000251558100016

  View details for PubMedID 17686607