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  • Molecular Characterization of Aggressive Estrogen Receptor Positive Breast Cancer Resistant to Palbociclib Therapy Lei, L., Lin, C., Steiner, D., Ford, J. M., Zehnder, J. L., Suarez, C. J. NATURE PUBLISHING GROUP. 2018: 703
  • Patient and provider perspectives on the development of personalized medicine: a mixed-methods approach. Journal of community genetics Puryear, L., Downs, N., Nevedal, A., Lewis, E. T., Ormond, K. E., Bregendahl, M., Suarez, C. J., David, S. P., Charlap, S., Chu, I., Asch, S. M., Pakdaman, N., Chang, S. I., Cullen, M. R., Palaniappan, L. 2017

    Abstract

    While genetic testing gains adoption in specialty services such as oncology, neurology, and cardiology, use of genetic and genomic testing has yet to be adopted as widely in primary care. The purpose of this study is to identify and compare patient and primary care provider (PCP) expectations of genetics services in primary care. Patient and PCP perspectives were assessed through a mixed-method approach combining an online survey and semi-structured interviews in a primary care department of a large academic medical institution. A convenience sample of 100 adult primary care patients and 26 PCPs was gathered. The survey and interview questions focused on perceptions of genetic testing, experience with genetic testing, and expectations of genetic services in primary care. Patients felt that their PCP was knowledgeable about genetic testing and expected their PCP to be the first to recognize a need for genetic testing based on family history. Nonetheless, patients reported that PCPs rarely used family history information to discuss genetic risks or order testing. In contrast, PCPs felt uncertain about the clinical utility and scientific value of genetic testing. PCPs were concerned that genetic testing could cause anxiety, frustration, discrimination, and reduced insurability, and that there was unequal access to testing. PCPs described themselves as being "gatekeepers" to genetic testing but did not feel confident or have the desire to become experts in genetic testing. However, PCPs were open to increasing their working knowledge of genetic testing. Within this academic medical center, there is a gap between what patients expect and what primary care providers feel they are adequately prepared to provide in terms of genetic testing services.

    View details for DOI 10.1007/s12687-017-0349-x

    View details for PubMedID 29280052

  • Synchronous immunophenotypically and clonally distinct follicular lymphoma and marginal zone lymphoma with massive amyloid deposition HUMAN PATHOLOGY: CASE REPORTS Frost, A. S., Suarez, C. J., Kieckbusch, M. E., Burtelow, M., Natkunam, Y. 2016; 5: 10-17
  • Next-Generation Sequencing for Infectious Disease Diagnosis and Management A Report of the Association for Molecular Pathology JOURNAL OF MOLECULAR DIAGNOSTICS Lefterova, M. I., Suarez, C. J., Banaei, N., Pinsky, B. A. 2015; 17 (6): 623-634

    View details for DOI 10.1016/j.jmoldx.2015.07.004

    View details for Web of Science ID 000363830000001

    View details for PubMedID 26433313

  • Lymph node involvement by mycosis fungoides and Sezary syndrome mimicking angioimmunoblastic T-cell Lymphoma HUMAN PATHOLOGY LeBlanc, R. E., Lefterova, M. I., Suarez, C. J., Tavallaee, M., Kim, Y. H., Schrijver, I., Kim, J., Gratzinger, D. 2015; 46 (9): 1382-1389

    Abstract

    Clinical management of cutaneous T-cell lymphoma (CTCL) and angioimmunoblastic T-cell lymphoma (AITL) differs markedly. Diagnostic distinction is critical. Herein, we describe a series of 4 patients with clinically, molecularly, and histopathologically annotated mycosis fungoides or Sézary syndrome whose nodal disease mimicked AITL. The patients otherwise exhibited classic clinical manifestations of mycosis fungoides/Sézary syndrome preceding the onset of lymphadenopathy by 1 to 5 years. Skin biopsies revealed epidermotropic infiltrates characteristic of CTCL. Lymph node biopsies revealed dense CD4+ T-cell infiltrates that coexpressed follicular helper T-cell markers and were accompanied by proliferations of high endothelial venules and arborizing CD21+ follicular dendritic cell networks. Two patients had T-cell receptor gene rearrangement studies performed on their skin, lymph node, and peripheral blood demonstrating identical polymerase chain reaction clones in all 3 tissues. A small secondary clonal B-cell population was present in 1 patient that mimicked the B-cell proliferations known to accompany AITL and persisted on successive nodal biopsies over several years. This latter phenomenon has not previously been described in CTCL. The potential for patients to be misdiagnosed with AITL for lack of consideration of advanced-stage CTCL with nodal involvement underscores the necessity of information sharing among the various pathologists and clinicians involved in the care of each patient.

    View details for DOI 10.1016/j.humpath.2015.05.024

    View details for Web of Science ID 000360779200017

  • Mycotic aneurysm of the abdominal aorta caused by Haemophilus influenzae type f JOURNAL OF MEDICAL MICROBIOLOGY Suarez, C. J., Glover, W. A., Cowan, J., Smith, A., Clarridge, J. E. 2013; 62: 658-660

    Abstract

    Prior to the advent of the Haemophilus influenzae type b vaccine, invasive infections due to H. influenzae type f were rarely described. However, the epidemiology of H. influenzae is changing. While the incidence of invasive infections due to H. influenzae is declining in children, such infections are becoming more common in adults, particularly in the elderly. Here, we report an unusual case of infective aortic aneurysm caused by H. influenzae type f that underlines the emerging clinical relevance and pathogenic capability of this organism.

    View details for DOI 10.1099/jmm.0.055228-0

    View details for Web of Science ID 000318203100023

    View details for PubMedID 23355310

  • Actions and consequences: characterization of a deletion in the cftr gene that encompasses a splice site Grand Rounds Suarez, C. J., Boyle, T., Chiang, T., Schrijver, I. 2013; 12: 36-39
  • Genotypic and phenotypic characterization and clinical significance of 'Haemophilus quentini' isolated from the urinary tract of adult men JOURNAL OF MEDICAL MICROBIOLOGY Glover, W. A., Suarez, C. J., Clarridge, J. E. 2011; 60 (11): 1689-1692

    Abstract

    'Haemophilus quentini' has been proposed as the name for a distinct and homogeneous Haemophilus genospecies associated with urogenital tract and neonatal-related infections. Reports of 'H. quentini' isolation from adult men are rare and the disease potential in this population is unknown. We report six cases where 'H. quentini' was isolated from the genito-urinary tract in males. The isolation of 'H. quentini' during routine urine and urethral culture in adult men may aid in the determination of unresolved urethritis and possible urinary tract infections.

    View details for DOI 10.1099/jmm.0.031591-0

    View details for Web of Science ID 000296547900017

    View details for PubMedID 21737543

  • Genetic biomarkers in colorectal cancer Clinical Laboratory International Suarez, C. J., Konnick, E. Q., Pritchard, C. 2011; 35: 26-28
  • Respiratory Comparative anatomy and histology: A mouse and human atlas Suarez, C. J., Frevert, C. W., Dintzis, S. M. Elsevier. 2011; 1st: 121–134
  • Surfactant Protein D-Mediated Decrease of Allergen-Induced Inflammation Is Dependent upon CTLA4 JOURNAL OF IMMUNOLOGY Lin, K., Jen, K. Y., Suarez, C. J., Crouch, E. C., Perkins, D. L., Finn, P. W. 2010; 184 (11): 6343-6349

    Abstract

    Pulmonary surfactant protein D (SP-D), a member of the collectin family, is an innate immune molecule critical for defense that can also modulate adaptive immune responses. We previously showed that SP-D-deficient mice exhibit enhanced allergic responses and that SP-D induction requires lymphocytes. Thus, we postulated that SP-D may decrease adaptive allergic responses through interaction with T cells. In this study, we used two forms of SP-D, a dodecamer and a shorter fragment containing the trimeric neck and carbohydrate recognition domains (SP-D NCRD). Both forms decreased immune responses in vitro and in a murine model of pulmonary inflammation. SP-D NCRD increased transcription of CTLA4, a negative regulator of T cell activation, in T cells. SP-D NCRD no longer decreased lymphoproliferation and IL-2 cytokine production when CTLA4 signals were abrogated. Administration of SP-D NCRD in vivo no longer decreased allergen induced responses when CTLA4 was inhibited. Our results indicate that SP-D decreases allergen responses, an effect that may be mediated by increase of CTLA4 in T cells.

    View details for DOI 10.4049/jimmunol.0901947

    View details for Web of Science ID 000278439600049

    View details for PubMedID 20435925

  • T Cell Pathways Involving CTLA4 Contribute To a Model of Acute Lung Injury JOURNAL OF IMMUNOLOGY Nakajima, T., Suarez, C. J., Lin, K., Jen, K. Y., Schnitzer, J. E., Makani, S. S., Parker, N., Perkins, D. L., Finn, P. W. 2010; 184 (10): 5835-5841

    Abstract

    Acute lung injury (ALI) is a frequent pulmonary complication in critically ill patients. We characterized a murine model of LPS-induced ALI, focusing on Th cells. Following LPS administration, bronchoalveolar lavage lymphocytes, neutrophils, IL-6, TNF-alpha, and albumin were increased. Analysis of LPS-induced T cells revealed increased Th cell-associated cytokines (IL-17A, -17F, and -22), as well as increased expression of CD69 (a cell activation marker), Foxp3, and CTLA4 in CD4(+) T cells. Administration of anti-CTLA4 Ab decreased LPS-induced bronchoalveolar lavage albumin and IL-17A, while increasing CD4(+)Foxp3(+) cell number and Foxp3 expression in CD4(+)Foxp3(+) cells. These data suggest that pulmonary LPS administration promotes CD4(+) T cells and that T cell pathways involving CTLA4 contribute to ALI.

    View details for DOI 10.4049/jimmunol.0903238

    View details for Web of Science ID 000277530700047

    View details for PubMedID 20385880

  • Innate immune mechanism in allergic asthma CURRENT ALLERGY AND ASTHMA REPORTS Suarez, C. J., Parker, N. J., Finn, P. W. 2008; 8 (5): 451-459

    Abstract

    Allergic asthma is a chronic inflammatory disorder of the airways characterized by eosinophilic inflammation, airway hyperresponsiveness, and mucus hypersecretion. Adaptive, antigen-dependent immunity is critical for asthma pathogenesis. Allergic asthma may involve adaptive and innate, antigen-independent immune responses. This review discusses the current evidence that associates innate immunity with allergic asthma pathogenesis. In particular, we focus on the role of innate immune cells (eg, bronchial epithelial cells, alveolar macrophages, and dendritic cells) and molecules (Toll-like and nucleotide-binding oligomerization domain-like receptors) in modifying allergic immune responses.

    View details for Web of Science ID 000258547500013

    View details for PubMedID 18682113

  • First detection of the plasmid-mediated class A carbapenemase KPC-2 in clinical isolates of Klebsiella pneumoniae from South America ANTIMICROBIAL AGENTS AND CHEMOTHERAPY Villegas, M. V., Lolans, K., Correa, A., Suarez, C. J., Lopez, J. A., Vallejo, M., Quinn, J. P. 2006; 50 (8): 2880-2882

    Abstract

    The plasmid-mediated class A carbapenemase KPC-2 was isolated from unrelated Klebsiella pneumoniae isolates in Medellin, Colombia. These KPC enzymes are the first from South America and the second isolation outside of the United States. The expanding geographic spread of KPC carbapenemases underscores the importance of clinical recognition of these enzymes.

    View details for DOI 10.1128/AAC.00186-06

    View details for Web of Science ID 000239640400047

    View details for PubMedID 16870793

  • Mechanism of resistance to Carbapenems in Enterobacteriaceae, P.aeruginosa and A.baumannii, and some strategies for containment Infectio Suarez, C. J., Kattan, J. N., Villegas, M. V. 2006; 10 (2): 69-77
  • First detection of metallo-beta-lactamase VIM-2 in Pseudomonas aeruginosa isolates from Colombia ANTIMICROBIAL AGENTS AND CHEMOTHERAPY Villegas, M. V., Lolans, K., Olivera, M. D., Suarez, C. J., Correa, A., Queenan, A. M., Quinn, J. P. 2006; 50 (1): 226-229

    Abstract

    Carbapenem resistance rates in Pseudomonas aeruginosa isolates in Colombia, as in many South American countries, are high for reasons that remain unclear. From our nationwide network, we describe the first detection of the metallo-beta-lactamase VIM-2 in clinical isolates of P. aeruginosa from multiple cities within Colombia. Metallo-beta-lactamases were not detected in the two centers with the highest imipenem resistance rates. Clonality was noted in five of the eight centers with strains meeting the criteria for molecular typing. The high carbapenem resistance in P. aeruginosa in Colombia may be attributable to a combination of factors, including the presence of metallo-beta-lactamases and nosocomial transmission.

    View details for DOI 10.1128/AAC.50.1.226-229.2006

    View details for Web of Science ID 000234988000029

    View details for PubMedID 16377690

  • Mechanisms of resistance to beta-lactams in some common Gram-negative bacteria causing nosocomial infections. Expert review of anti-infective therapy Suárez, C. J., Lolans, K., Villegas, M. V., Quinn, J. P. 2005; 3 (6): 915-922

    Abstract

    Gram-negative bacilli remain major killers of hospitalized patients and continue to evolve new resistance mechanisms. This review describes the mechanisms of resistance to beta-lactam antibiotics from those Gram-negative pathogens most often isolated from nosocomial infections.

    View details for PubMedID 16307504