Human Finger-Prick Induced Pluripotent Stem Cells Facilitate the Development of Stem Cell Banking
STEM CELLS TRANSLATIONAL MEDICINE
Tan, H., Toh, C., Ma, D., Yang, B., Liu, T., Lu, J., Wong, C., Tan, T., Li, H., Syn, C., Tan, E., Lim, B., Lim, Y., Cook, S. A., Loh, Y.
2014; 3 (5): 586–98
Abstract
Induced pluripotent stem cells (iPSCs) derived from somatic cells of patients can be a good model for studying human diseases and for future therapeutic regenerative medicine. Current initiatives to establish human iPSC (hiPSC) banking face challenges in recruiting large numbers of donors with diverse diseased, genetic, and phenotypic representations. In this study, we describe the efficient derivation of transgene-free hiPSCs from human finger-prick blood. Finger-prick sample collection can be performed on a "do-it-yourself" basis by donors and sent to the hiPSC facility for reprogramming. We show that single-drop volumes of finger-prick samples are sufficient for performing cellular reprogramming, DNA sequencing, and blood serotyping in parallel. Our novel strategy has the potential to facilitate the development of large-scale hiPSC banking worldwide.
View details for DOI 10.5966/sctm.2013-0195
View details for Web of Science ID 000335939000015
View details for PubMedID 24646489
View details for PubMedCentralID PMC4006490
