Transformation of coral larvae
We have succesfully generated mosaic F0 larvae with mutations at target sites via microinjection delivery of CRISPR/Cas9 reagents into the single cell zygote. Open Access PNAS publication
Transformation of Aiptasia larvae
We have successfully generated larvae with overexpression contructs and knock-down larvae via electroporation of plasmids, mRNA, and shRNA into single cell zygotes.
CRISPR based gene editing
We have successfully introduced loss of function mutations into coral larvae. Full free PNAS article