JNM highlights MIPS research: New Compact and Low-Cost Lensless Radiomicroscope Developed for Nuclear Medicine Imaging

March 14, 2023

New research from Guillem Pratx's team has been published as a Featured Basic Science Article in The Journal of Nuclear Medicine.

News release: http://www.snmmi.org/NewsPublications/NewsDetail.aspx?ItemNumber=43587

Abstract: The action of radiopharmaceuticals takes place at the level of cells. However, existing radionuclide assays can only measure uptake in bulk or in small populations of single cells. This potentially hinders the development of effective radiopharmaceuticals for disease detection, staging, and treatment. Methods: We have developed a new imaging modality, the lensless radiomicroscope (LRM), for in vitro, cellular-resolution imaging of β- and α-emitting radionuclides. The palm-sized instrument is constructed from off-the-shelf parts for a total cost of less than $100, about 500 times less than the radioluminescence microscope, its closest equivalent. The instrument images radiopharmaceuticals by direct detection of ionizing charged particles via a consumer-grade complementary metal-oxide semiconductor detector. Results: The LRM can simultaneously image more than 5,000 cells within its 1 cm2 field of view, a 100-times increase over state-of-the-art technology. It has spatial resolution of 5 μm for brightfield imaging and 30 μm for 18F positron imaging. We used the LRM to quantify 18F-FDG uptake in MDA-MB-231 breast cancer cells 72 h after radiation treatment. Cells receiving 3 Gy were 3 times larger (mean = 3,116 μm2) than their untreated counterparts (mean = 940 μm2) but had 2 times less 18F-FDG per area (mean = 217 Bq/mm2), a finding in agreement with the clinical use of this tracer to monitor response. Additionally, the LRM was used to dynamically image the uptake of 18F-FDG by live cancer cells, and thus measure their avidity for glucose. Conclusion: The LRM is a high-resolution, large-field-of-view, and cost-effective approach to image radiotracer uptake with single-cell resolution in vitro.

Klein JS, Kim TJ, Pratx G. Development of a Lensless Radiomicroscope for Cellular-Resolution Radionuclide Imaging. J Nucl Med. 2023 Mar;64(3):479-484. doi: 10.2967/jnumed.122.264021. Epub 2022 Sep 15. PMID: 36109183.

Source cited: Journal of Nuclear Medicine

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