Peer-Reviewed Research Publications


105.        Khanna A, Oropeza BP, Huang NF. Cardiovascular human organ-on-a-chip platform for disease modeling, drug development, and personalized therapy. J Biomed Mater Res A  112:512-523, 2024. doi: 10.1002/jbm.a.37602.

106.        Bashur CA, Huang NF. Special issue: Frontiers in biomaterials for cardiovascular health. J Biomed Mater Res A. 112:490-491, 2024. doi: 10.1002/jbm.a.37658. Epub 2023

107.        Huang NF, Stern B, Oropeza BP, Zaitseva TS, Paukshto MV, Zoldan J. Bioengineering Cell Therapy for Treatment of Peripheral Artery Disease. Arterioscler Thromb Vasc Biol. 44:e66-e81, 2024. doi: 10.1161/ATVBAHA.123.318126. *featured as journal cover


104.       Shayan M, Huang MS, Navarro R, Chiang G, Hu C, Oropeza BP, Johansson PK, Suhar RA, Foster AA, LeSavage BL, Zamani M, Enejder A, Roth JG, Heilshorn SC, Huang NF. Elastin-like protein hydrogels with controllable stress relaxation rate and stiffness modulate endothelial cell function. J Biomed Mater Res A. 11:896-909, 2023.

103.       Chan AHP, Hu C, Chiang G, Ekweume C, Huang NF. Chronic Nicotine Impairs the Angiogenic Capacity of Human Induced Pluripotent Stem Cell-Derived Endothelial Cells in a Murine Model of Peripheral Arterial Disease. JVS Vasc Sci, 4: 100115, 2023,

102.       Chan A.H.P., Jain I, Oropeza BP, Zhou T, Nelsen B, Geisse NA, Huang NF. Combinatorial extracellular matrix cues with mechanical strain induce differential effects on myogenesis in vitro. Biomater Sci, 11:5893-5907, 2023.

101.       Nguyen D, Dionyssiou D, Zaitseva TS, Zhou A, Sue G, Deptula P, Moroz MA, Tabada P, Rockson SG, Paukshto MV, Cheng MH, Huang NF. Development of A Rat Model of Lymphedema and the Implantation of a Collagen-Based Medical Device for Therapeutic Intervention. Front Cardiovasc Med  10:1214116. eCollection 2023.

100.       Khanna A, Oropeza BP, Huang NF. Engineering Spatiotemporal Control in Vascularized Tissues. Bioengineering  9: 555, 2022.

99.  Huang NF, Zaitseva TS, Paukshto MV. Biomedical Applications of Collagen. Bioengineering 10: 90, 2023.  

98.  Jain I, Oropeza BP, Huang NF. Multiomics Analyses of Peripheral Artery Disease Muscle Biopsies. Circ Res. 2023 May 26;132:1444-1446, 2023.

97.  Khanna A, Oropeza BP, Huang NF. Cardiovascular human organ-on-a-chip platform for disease modeling, drug development, and personalized therapy. J Biomed Mater Res A  2023 Sep 5. (epub)


96.  Eugenis I, Wu D, Hu C, Chiang G, Huang NF, Rando TA. Scalable macroporous hydrogels enhance stem cell treatment of volumetric muscle loss. Biomaterials, 290:121818, 2022.

95.  Zamani M, Cheng Y-H, Charbonier F, Gupta VK, Mayer AT, Trevino AE, Quertermous T, Chaudhuri O, Cahan P, Huang NF. Single-Cell Transcriptomic Census of Endothelial Changes Induced by Matrix Stiffness and the Association with Atherosclerosis. Adv Funct Mater, 32: 2203069, 2022.

94.  Khanna A, Ayan B, Huang NF. Advances In Three-Dimensional Bioprinted Stem Cell-Based Tissue Engineering for Cardiovascular Regeneration. J Mol Cell Cardiol 169:13-27, 2022.

93.  Wang L, Xu G, Pan L, Aikawa E, Aikawa M, Xiao J, Huang NF. Editorial: Frontiers in Cardiovascular Medicine: Rising Stars 2021. Front. Cardiovasc. Med. 9:928981, 2022.

92.  Park YB, Lin S, Bai Y, Moeinzadeh S, Kim S, Huang J, Lee U, Huang NF, Yang YP. Dual delivery of BMP-2 and IGF-1 through injectable hydrogel promotes cranial bone defect healing. Tissue Eng Part A, 28(17-18):760-769, 2022.

91. Hu C, Ayan B, Chiang G, Chang AHP, Rando TA, Huang NF. Comparative Effects of Basic Fibroblast Growth Factor Delivery or Voluntary Exercise on Muscle Regeneration after Volumetric Muscle Loss. Bioengineering 9: 37 2022.  From the Special Issue, Extracellular Matrix in Musculoskeletal Regeneration.


90. Mulorz J, Shayan M, Hu C, Alcazar C, Chan AHP, Briggs M, Wen Y, Walvedar AP, Ramasubramanian A, Spin JM, Chen B, Tsao PS, Huang NF. Peri-Adventitial Delivery of Smooth Muscle Cells in Porous Collagen Scaffolds For Treatment of Experimental Abdominal Aortic Aneurysm. Biomater Sci 9: 6903–6914, 2021.

89.  Zhang H, Bouman-Chen Z, Fredman G, Gomez D, Gumbach IM, Huang NF, Nguyen P, Oumet M, Sutton N, Aikawa E. What Makes a Great Mentor: Interviews with Recipients of the ATVB Mentor of Women Award. Arterioscler Thromb Vasc Biol, 41:2641-2647, 2021.

88. Khanna A, Zamani M, Huang NF. ECM-Based Biomaterials for Cardiovascular Tissue Engineering

87.  Chan AHP, Huang NF. Engineering cardiovascular tissue chips for disease modeling and drug screening applications. Front Bioeng Biotechnol, 9:673212, 2021. doi: 10.3389/fbioe.2021.673212 (invited review for special collection on “Bioengineering and Biotechnology Approaches in Cardiovascular Sciences”)

86.  Agarwal T, Fortunato GM, Hann SY, Ayan B, Vajanthri KY, Presutti D, Cui H, Chan AHP, Costantini M, Onesto V, Di Natale C, Huang NF, Makvandi P, Shabani M, Maiti TK, Zhang LG, De Maria C. Recent Advances in Bioprinting Technologies for Engineering Cardiac Tissue. Mat Sci Eng C, 124:112057, 2021.

85. Liang G, Huang X, Hirsh J, Mehmi S, Fonda H, Chan K, Huang NF, Aalami O, Froelicher V, Lee DP, Myers J, Lee AS, Nguyen PK. Modest gains after an 8-week exercise program correlate with reductions in nontraditional markers of cardiovascular risk. Front Cardiovasc Med 8:669110, 2021.


84.  Alcazar C, Hu C, Rando TA, Huang NF#, Nakayama KH. Transplantation of Insulin-like Growth Factor-1 Laden Scaffolds Combined with Exercise Promotes Neurovascular Regeneration and Angiogenesis in a Muscle Injury Preclinical Model. Biomater Sci 2020, epub (# co-corresponding author)

83.  Huang NF, Chaudhuri O, Cahan P, Wang A, Engler AJ, Wang Y, Kumar S, Khademhosseini A, Li S. Multi-scale cellular engineering: From molecules to organ-on-a-chip. APL Bioeng 4:010906, 2020.

82.  Chan AHP, Huang NFEffects of nicotine on the translation of stem cell therapy. Regen Med 15:1679-1688.

81.  Shayan M, Huang NF. Pre-Clinical Cell Therapeutic Approaches for Repair of Volumetric Muscle Loss. Bioengineering. 7, 97, 2020, epub. (Part of special issue on Advances in Skeletal Muscle Tissue Engineering)

80.  Hu C, Zaitseva TS, Alcazar C, Tabada P, Sawamura S, Yang G, Borrelli MR, Wan DC, Nguyen DH, Paukshto MV, Huang NF. Delivery of Human Stromal Vascular Fraction Cells on Nanofibrillar Scaffolds for Treatment of Peripheral Arterial Disease. Front Bioeng Biotechnol 8:689, 2020. eCollection 2020.

79.  Zaitseva TS, Yang G, Dionyssiou D, Zamani M, Sawamura S, Yakubov E, Ferguson J, Hallet RL, Fleischmann D, Paukshto MV, Huang NF. Delivery of hepatocyte growth factor mRNA from nanofibrillar scaffolds in a pig model of peripheral arterial disease. Regen Med 15:1761-1773, 2020.


78. Wanjare M, Huang NF. Stem Cells: Efficacy In Peripheral Vascular Diseases.  In: The Encyclopedia of Tissue Engineering and Regenerative Medicine (Ed: David Warburton). Volume 2, p181-187. Elsevier Inc, Waltham, MA, 2019 (ISBN-13: 978-0128136997, ISBN-10: 012813699)

77. Wanjare M, Kawamura M, Hu C, Alcazar C, Wang H, Woo YJ, Huang NF. Vascularization of engineered spatially patterned myocardial tissue derived from human pluripotent stem cells in vivo. Front Bioeng Biotechnol 7:208, 2019.  doi: 10.3389/fbioe.2019.00208.

76. Zaitseva T, Alcazar C, Zamani M, Hou L, Sawamura S, Yakubov E, Hopkins M, Woo YJ, Paukshto M, Huang NF. Aligned Nanofibrillar Scaffolds for Controlled Delivery of Modified mRNA. Tissue Eng Part A. 25:121-130, 2019. doi: 10.1089/ten.TEA.2017.0494.

75. Nakayama KH, Shayan M, Huang NF. Engineering Biomimetic Materials for Skeletal Muscle Repair and Regeneration. Adv Healthc Mater. 8:e1801168, 2019. doi: 10.1002/adhm.201801168.

74. Charbonier FW, Zamani M, Huang NF. Endothelial Cell Mechanotransduction in the Dynamic Vascular Environment. Adv Biosystems, 1800252, 2019. doi: 10.1002/adbi.201800252.

73. Nakayama KH, Quarta M, Paine P, Alcazar C, Karakikes I, Garcia V, Abilez O, Calvo NS, Simmons CS, Rando TA, Huang NF. Treatment of Volumetric Muscle Loss Using Spatially Patterned Scaffolds Enhances Vascular Organization and Functional Integration. Commun Biol  2:170, 2019. eCollection 2019.


72. Zamani M, Karaca E, Huang NF. Multicellular Interactions in 3D Engineered Myocardial Tissue. Front Cardiovasc Med. 5:147, 2018. doi: 10.3389/fcvm.2018.00147.

71. Huang NF, Serpooshan V, Morris VB, Sayed N, Pardon G, Abilez OJ, Nakayama KH, Pruitt BL, Wu SM, Yoon Y, Zhang J, Wu JC. Big bottlenecks in cardiovascular tissue engineering. Commun Biol 1:199, 2018. doi: 10.1038/s42003-018-0202-8. 

70. Nakayama KH, Alcazar C, Yang G, Quarta M, Paine P, Doan L, Davis A, Rando TA, Huang NF. Rehabilitative Exercise and Spatially Patterned Nanofibrillar Scaffolds Enhance Vascularization and Innervation Following Volumetric Muscle Loss. npj Regen Med, 3:16, 2018. doi: 10.1038/s41536-018-0054-3.

69. Hou L, Yang G, Tang S, Alcazar C, Joshi P, Strassberg Z, Kim M, Kawamura M, Woo YJ, Shrager J, Ding S, Huang NF. A small molecule derived from carboxyethylpyrrole protein adducts promotes angiogenesis in a mouse model of peripheral arterial disease. JAHA 7(18):e009234, 2018.  DOI: 10.1161/JAHA.118.009234.

68. Foster AA, Dewi RE, Cai L, Hou L, Strassberg Z, Alcazar CA, Heilshorn SC, Huang NF. Protein-engineered hydrogels enhance the survival of induced pluripotent stem cell-derived endothelial cells for treatment of peripheral arterial disease. Biomater Sci. 6:614-622, 2018

67. Ma Z, Zhang M, Yue J, Alcazar C, Zhong Y, Doyle TC, Dai H, Huang NF. Near-Infrared IIb Fluorescence Imaging of Vascular Regeneration with Dynamic Tissue Perfusion Measurement and High Spatial Resolution. Adv Funct Mater, 28: 1803417, 2018.

66.  Huang NF, Nicolas L’Heureux, Song Li (Editors). Engineering Stem Cells for Tissue Regeneration. World Scientific Publishing Co: NY, 2018 (ISBN: 978-981-3147-74-4)

65. Guang Yang, Huang NF, Li S. Mesenchymal Stem Cells for Tissue Regeneration. In: Engineering Stem Cells for Tissue Regeneration. World Scientific Publishing Co: NY, 2018

64. Hou L, Huang NF. Stem Cell-Derived Endothelial Cells for Cardiovascular Regeneration. In: Engineering Stem Cells for Tissue Regeneration. World Scientific Publishing Co: NY, 2018

63.  Zhong Y, Ma Z, Zhu S, Yue J, Zhang M, Antaris AL, Yuan J, Cui R, Wan H, Zhou Y, Wang W, Huang NF, Luo J, Hu Z, Dai H. Boosting the down-shifting luminescence of rare-earth nanocrystals for biological imaging beyond 1500 nm. Nat Commun. 2017 8:737.


62.  Clayton ZE, Yuen GSC, Sadeghipour S, Hywood JD, Wong JWT, Huang NF, Ng MKC, Cooke JP, Patel S. A comparison of the pro-angiogenic potential of human induced pluripotent stem cell derived endothelial cells and induced endothelial cells in a murine model of peripheral arterial disease.  Int J Cardiol, 234:81-89, 2017.

61.  Wanjare M, Hou L, Nakayama KH, Kim JJ, Mezak NP, Abilez OJ, Tzatzalos E, Wu JC, Huang NF. Anisotropic microfibrous scaffolds enhance the organization and function of cardiomyocytes derived from induced pluripotent stem cells. Biomat Sci.   5: 1567–1578, 2017.

60.  Hou L, Kim JJ, Wanjare M, Patlolla B, Coller J, Natu V, Hastie TJ, Huang NF. Combinatorial Extracellular Matrix Microenvironments for Probing Endothelial Differentiation of Human Pluripotent Stem Cells. Sci Rep 7, 6551, 2017.

59. Kim JJ, Hou L, Yang G, Mezak NP, Wanjare M, Joubert LM, Huang NF. Microfibrous Scaffolds Enhance Endothelial Differentiation and Organization of Induced Pluripotent Stem Cells. Cell Mol Bioeng, 2017, 10:417-432, 2017.

58.  Wanjare M, Huang NF. Regulation of the Microenvironment for Cardiac Tissue Engineering. Regen Med. 12:187-201, 2017.

57.  Carcamo-Orive I, Huang NF, Quertermous T, Knowles JW. iPSC-derived endothelial cells in insulin resistance and metabolic syndrome. Arterioscler Thromb Vasc Biol, 2017. 37:2038-2042, 2017.


56. Yuan K, Orcholski ME, Huang NF, de Jesus Perez VA. In Vivo Study of Human Endothelial-Pericyte Interaction Using the Matrix Gel Plug Assay in Mouse. J Vis Exp. 118, 2016.

55.  Hou L, Coller J, Natu V, Hastie TJ, Huang NF. Combinatorial Extracellular Matrix Microenvironments Promote Survival and Phenotype of Human Induced Pluripotent Stem Cell-Derived Endothelial Cells in Hypoxia. Acta Biomater. 44: 199-199, 2016. 

54.  Deveza L, Choi J, Lee J, Huang N, Cooke J, Yang F. Polymer-DNA nanoparticle-induced CXCR4 overexpression improves stem cell engraftment an tissue regeneration in a mouse hindlimb ischemia model. Theranostics 6:1176-1189, 2016.

53.  Hadamitzky C, Zaitseva TS, Bazalova-Carter M, Paukshto MV, Hou L, Strassberg Z, Ferguson J, Matsuura Y, Dash R, Yang PC, Kretchetov S, Vogt PM, Rockson SG, Cooke JP, Huang NF. Aligned nanofibrillar collagen scaffolds - Guiding lymphangiogenesis for treatment of acquired lymphedema. Biomaterials 102:259-67, 2016.

52.  Kim JJ, Hou L, Huang NF. Vascularization of three-dimensional engineered tissues for regenerative medicine applications. Acta Biomater. 41:17-26, 2016.

51.  Ogle BM, Bursac N, Domian I, Huang NF, Menasché P, Murry CE, Pruitt B, Radisic M, Wu JC, Wu SM, Zhang J, Zimmermann WH, Vunjak-Novakovic G. Distilling complexity to advance cardiac tissue engineering.  Sci Transl Med. 8: 342ps13, 2016.

50.  Hou L, Coller J, Natu V, Hastie TJ, Huang NF. Combinatorial Extracellular Matrix Microenvironments Promote Survival and Phenotype of Human Induced Pluripotent Stem Cell-Derived Endothelial Cells in Hypoxia. Acta Biomater. S1742-706130390-7, 2016.

49.  Giordano S, Zhao X, Xing D, Hage F, Oparil S, Cooke JP, Lee J, Nakayama KH, Huang NF, Chen Y-F. Targeted Delivery of Human iPS-ECs Overexpressing IL-8 Receptors Inhibits Neointimal and Inflammatory Responses to Vascular Injury in the Rat. Am J Physiol Heart Circ Physiol.  Epub, 2016.

48. Nakayama KH, Surya VN, Gole M, Walker TW, Yang W, Lai ES, Ostrowski MA, Fuller GG, Dunn AR, Huang NFNanoscale Patterning of Extracellular Matrix Alters Endothelial Function under Shear Stress.  Nano Lett, 16:410-9, 2016.

47. Hou L, Kim JJ, Woo YJ, Huang NF. Stem Cell-Based Therapies to Promote Angiogenesis in Ischemic Cardiovascular Disease. Am J Physiol Heart Circ Physiol. 310:H455-65, 2016.


46. Dash R, Kim PJ, Matsuura Y, Ikeno F, Metzler S, Huang NF, Lyons JK, Nguyen PK, Ge X, Wong Po Foo C, McConnell MV, Wu JC, Yeung AC, Harnish P, Yang PC.  Manganese-Enhanced Magnetic Resonance Imaging Enables In Vivo Confirmation of Peri-Infarct Restoration Following Stem Cell Therapy in a Porcine Ischemia-Reperfusion Model. J Am Heart Assoc,   4: pii: e002044. doi: 10.1161/JAHA.115.002044, 2015.

45. Nakayama KH, Joshi PA, Lai ES, Gujar P, Joubert L-M, Chen B, Huang NF. Bi-layered vascular graft derived from human induced pluripotent stem cells with biomimetic structure and function. Regen Med 10:745-55, 2015.

44. Nakayama KH, Hong G, Lee JC, Patel J, Edwards B, Zaitseva TS, Paukshto MV, Dai H, Cooke JP, Woo YJ, Huang NF. Aligned-Braided Nanofibrillar Scaffold with Endothelial Cells Enhances Arteriogenesis.  ACS Nano, 9: 6900–6908, 2015

43. Yuan K, Orcholski M, Panaroni C, Shuffle EM, Huang NF, Jiang X, Tian W, Vladar EK, Wang L, Nicolls MR, Wu J, de Jesus Perez VA. Activation of the Wnt/Planar Cell Polarity Pathway Is Required for Pericyte Recruitment during Pulmonary Angiogenesis. Am J Pathol 20185:69-84, 2015. 


42. Burridge PW, Metzler SA, Nakayama K, Abilez OJ, Simmons CS, Bruce MA, Matsuura Y, Kim P, Wu JC, Butte M, Huang NF, Yang PC. Multi-cellular Interactions Sustain Long-Term Contractility of Human Pluripotent Stem Cell-Derived CardiomyocytesAm J Trans Res, 6:724-735, 2014.

41. Mulyasasmita W, Cai L, Dewi RE, Jha A, Ullmann SD, Luong RH, Huang NF, Heilshorn SC. Avidity-controlled hydrogels for injectable co-delivery of induced pluripotent stem cell-derived endothelial cells and growth factors. J Control Release 191:71-81, 2014

40. Nakayama K, Hou L, Huang NF. Role of Extracellular Matrix Signaling Cues in Modulating Cell Fate Commitment for Cardiovascular Tissue Engineering. Adv Healthc Mater 3:628-41, 2014.

39. Ghebremariam YT, Huang NF, Kambhampati S, Volz KS, Joshi GG, Anslyn EV, Cooke JP. Characterization of a Fluorescent Probe for Imaging Nitric Oxide. J Vasc Surg, 51:68-79, 2014.

38. Ostrowski MA, Huang NF, Walker TW, Verwijlen T, Poplawski C, Khoo AS, Cooke JP, Fuller GG, Dunn AR. Microvascular Endothelial Cells Migrate Upstream and Align Against the Shear Stress Field Created by Impinging Flow. Biophys J 106:366-374, 2014.

37. Hong G, Lee JC, Jha A, Diao S, Nakayama KH, Hou L, Doyle TC, Robinson JT, Antaris AL, Dai H, Cooke JP, Huang NF.  Near-Infrared II Fluorescence for Imaging Hindlimb Vessel Regeneration with Dynamic Tissue Perfusion Measurement. Circ Cardiovasc Imaging, 7:517-25, 2014.


36. Huang NF, Dewi RE, Okogbaa J, Lee JC, Rufaihah AJ, Heilshorn SC, Cooke, JC. Chemotaxis of human induced pluripotent stem cell-derived endothelial cells. Am J Transl Res 5:510-520, 2013. (*co-first author).

35. Bakr A, Pak O, Taye A, Hamada F, Hemeida R, Janssen W, Gierhardt M, Ghofrani HA, Seeger W, Grimminger F, Schermuly RT, Witzenrath M, Brandes RP, Huang N, Cooke JP, Weissmann N, Sommer N. Effects of dimethylarginine dimethylaminohydrolase-1 overexpression on the response of the pulmonary vasculature to hypoxia.  Am J Respir Cell Mol Biol. 49:491-500, 2013.

34. Jalil RA, Huang NF*, Kim J, Herold J, Volz KS, Park TS, Lee JC, Zambidis ET, Reijo-Pera R, Cooke, JP. Human Induced Pluripotent Stem Cell-Derived Endothelial Cells Exhibit Functional Heterogeneity. Am J Transl Res 5:21-35, 2013. (*co-first author).

33. Huang NF, Lai E, Fuller GG, Ribeiro AS, Pan S, Pruitt B, Fuller GG, Cooke JP. Spatial patterning and shear stress modulate endothelial cell morphology, gene expression, and function: implications for vascular tissue engineering. Biomaterials 34: 2928-2937, 2013.

32. Huang NF, Okogbaa JN, Lee JC, Paukshto M, Zaitseva T, Cooke JP. The Modulation of Endothelial Cell Morphology, Function, and Survival Using Anisotropic Nanofibrillar Collagen Scaffolds. Biomaterials, 34: 4038-4047, 2013.

31. Li J, Huang NF*, Zou J, Laurent TJ, Lee JC, Okogbaa J, Cooke JP, Ding S. Conversion of Human Fibroblasts to Functional Endothelial Cells by Defined Factors. Arterioscler Thromb Vasc Biol 33:1366-75, 2013. (*co-first author).


30. Huang NF, Okogbaa J, Babakhanyan A, Cooke JP. Bioluminescence Imaging of Stem Cell Survival for Vascular Regeneration. Theranostics 2:346-54, 2012.

29. Hong G, Lee JC, Robinson JT, Raaz U, Xie L, Huang NF#, Cooke JP, Dai H. Multi-Functional In Vivo Vascular Imaging Using Near-Infrared II Fluorescence. Nature Medicine, 18:1841-6, 2012 (#co-corresponding author).

28. Lai E, Huang NF*, Cooke JP, Fuller GG. Aligned nanofibrous vascular grafts modulate endothelial cell orientation and migration. Regen Med 7:649-661, 2012 (*co-first author).

27. Wong W, Huang NF, Botham CM, Sayed N, Cooke JP. Endothelial Cells Derived From Nuclear Reprogramming. Circ Res 111:1363-1375, 2012.

Before 2012

26. Abdul Jalil R, Huang NF*, Jame S, Lee J, Nguyen HN, Byers B, De A, Okogbaa J, Rollins MD, Reijo-Pera R, Gambhir SS, Cooke JP. Endothelial Cells Derived From Human iPSCs Increase Capillary Density and Improve Perfusion in a Mouse Model of Peripheral Arterial Disease. Arterioscler Thromb Vasc Biol,31:e72-79, 2011 (*co-first author).

25. Huang NF, Li S. Regulation of the matrix microenvironment for stem cell engineering and regenerative medicine. Annals Biomed Eng 39:1201-1214, 2011.

24. Huang NF, Kurpinski K, Fang Q, Lee RJ, Li S. Proteomic Identification of Biomarkers of Vascular Injury.  Am J Transl Res 3:139-148, 2011.

23. Huang NF, Patlolla B, Abilez OA, Sharma H, Beygui RE, Zarins CK, Cooke JP. A Matrix Micropatterning Platform for Cell Localization and Stem Cell Fate Determination. Acta Biomaterialia 6: 4614-4621, 2010.

22. Huang NF, Niiyama H, Peter C, De A, Natkunam Y, Fleissner F, Li Z, Rollins MD, Wu JC, Gambhir SS, Cooke JP. Embryonic Stem Cell-Derived Endothelial Cells Engraft into the Ischemic Hindlimb and Restore Perfusion. Arterioscler Thromb Vasc Biol 30:984-91, 2010.

21. Huang NF, Fleissner F, Sun J, Cooke JP.  Role of Nitric Oxide Signaling in Endothelial Differentiation of Embryonic Stem Cells. Stem Cells Dev 19:1617-1625, 2010.

20. Huang NF, Chu J, Lee RJ, Li S. Biophysical and Chemical Effects of Fibrin on Mesenchymal Stromal Gene Expression. Acta Biomaterialia 6: 3947-3956, 2010.

19. Huang NF, Lee RJ, Li S. Engineering of Aligned Skeletal Muscle by Micropatterning. Am J Transl Res, 2:19-31, 2010.

18. Yu J,  Huang NF*, Wilson KD, Velotta JB, Huang M, Li Z, Lee A, Robbins RC, Cooke JP, Wu JC. nAChRs Mediate Human Embryonic Stem Cell-Derived Endothelial Cells: Proliferation, Apoptosis, and Angiogenesis. PLos One, 4:e7040, 2009 (*co-first author).

17. Huang NF, Lam A, Fang Qizhi, Sievers RE, Li S, Lee RJ. Bone Marrow-Derived Mesenchymal Stem Cells in Fibrin Augments Angiogenesis in the Chronically Infarcted Myocardium. Regen Med4:527-538, 2009. Pubmed

16. Huang NF, Niiyama H, De A, Gambhir SS, Cooke JP. Embryonic Stem Cell-Derived Endothelial Cells for Treatment of Hindlimb Ischemia. J Vis Exp 23:1034, 2009.

15. Niiyama H, Huang NF, Rollins MD, Cooke JP. Murine model of hindlimb ischemia. J Vis Exp23:1035, 2009. 

14. Huang NF, Li S. Mesenchymal Stem Cells for Vascular Regeneration. Regen Med 3:877-92, 2008.

13. Lee RJ, Fang Q, Davol PA, Gu Y, Sievers RE, Grabert RC, Gall JM, Tsang E, Yee MS, Fok H, Huang NF, Padbury JF, Larrick JW, Lum LG. Antibody Targeting of Stem Cells to Infarcted Myocardium. Stem Cells 25:712-717, 2007.

12. Park JS, Huang NF, Kurpinski KT, Patel S, Hsu S, Li S. Mesenchymal Stem Cells: Mechanobiology and cardiovascular therapies. Frontiers in Bioscience: 12:5098-5116, 2007.

11. Huang NF, Lee RJ, Li S. Chemical and Physical Regulation of Stem Cells and Progenitor Cells: Potential for Cardiovascular Tissue Engineering. Tissue Eng: 13:1809-1823, 2007.

10. Huang NF, Patel S, Thakar RG, Wu J, Hsiao BS, Chu B, Lee RJ, Li S.  Skeletal Muscle Morphogenesis on Micropatterned and Nanopatterned Biopolymers. Nano Letters 6:537-542, 2006.

9. Huang NF, Sievers RE, Park JS, Fang Q, Li S, Lee RJ.  A Rodent Model of Myocardial Infarction for Testing the Efficacy of Cells and Polymers for Myocardial Reconstruction. Nature Protocols1:1596-1609, 2006.

8. Li S, Huang NF, Hsu S.  Mechanotransduction in Endothelial Cell Migration. J Cell Biochem96:1110-1126, 2005.

7. Huang NF, Yu J, Sievers R, Li S, Lee RJ.  Injectable Biopolymers Enhance Angiogenesis after Myocardial Infarction. Tissue Eng 11:1860-1866, 2005.

6. Levenberg S, Huang NF, Lavik E, Rogers AB, Itskovitz-Eldor J, Langer R.  Differentiation of Human Embryonic Stem Cells on Three-Dimensional Polymer Scaffolds. Proc Natl Acad Sci U S A  100:12741-12746, 2003.

5. Eisenbud D, Huang NF, Luke S, Silberklang M.  Skin Substitutes and Wound Healing: Current Status and Challenges. Wounds 16: 2-17, 2004.

4. Pujar NS, Huang NF, Daniels CL, Dieter L, Gayton MG, Lee AL.  Based-Catalyzed Hydrolysis of Phosphodiester Bonds in Pneumococcal Polysaccharides. Biopolymers 75: 71-84, 2004.

3. Huang NF, Zac-Varghese S, Luke S. Apoptosis in Skin Wound Healing. Wounds 15:182-94, 2003.

2. Thakar RG, Ho F, Huang NF, Liepmann D, Li S. Regulation of Vascular Smooth Muscle Cells by Micropatterning.  Biochem Biophys Res Com  307:883-890, 2003.

1. Huang NF, Gupta M, Varghese S, Rao S, Luke S.  Detection of Numerical Chromosomal Abnormalities in Epithelial Ovarian Neoplasms by Fluorescence in situ Hybridization (FISH) and a Review of Current Literature. Appl Immunohistochem Mol Morphol 10: 187-193, 2002.

Textbook Chapters

5. Lee JC, Huang NF, Cooke JP. Hindlimb Ischemia. In: Manual of Research Techniques in Cardiovascular Medicine. Ed. Roberto Bolli. Wiley-Blackwell, NY, 2014 (ISBN# 0470672692)

4. Huang NF. Tissue Engineering and Regenerative Medicine: Role of Extracellular Matrix Microenvironment. In: Stem Cells and Cancer Stem Cells. Volume 9: 313-324, Springer, NY, 2013 (ISBN# 9400756445 )

3. Huang NF, Li S. Mesenchymal Stem Cells for Tissue Regeneration. In: Stem Cell and Tissue Engineering. World Scientific Publishing Co: NY, 2011 (ISBN# 9789814317054)

2. Huang NF, Gupta M, Luke S. Detection of Genetic Abnormalities in Ovarian Carcinoma. In:Immunohistochemistry and in situ Hybridization of Human Carcinomas. Vol 4, Elsevier Science Press, NY, 2006 (ISBN# 9780123339416)

1. Levenberg S, Huang NF, Itskovitz-Eldor J, Langer R.  Endothelial Cells Derived from Human Embryonic Stem Cells. In: Human ES Cells: Basic Biology and Multilineage Differentiation. Bios Scientific Publishers, Ltd., Oxford, UK, 2004. (ISBN# 9781859962787)



4. Huang NF, Zaitseva T, Paukshto M, Fuller GG, Cooke JP. A Graft For Directed Vascular And
Lymphatic Regeneration And Methods To Guide Endothelial Cell Assembly.  (US Patent Provisional Application Serial #61/627,289)

3. Hong G, Lee J, Huang NF, Cooke JP, Dai H. Vascular imaging using near infrared fluorescence (PCT/2012/66157)

Before 2012

2. Su J, Huang NF. Carbon Nanotube/fiber Reinforced Three Dimensionally Ordered Nano Scale Porous Carbon and the Process. This patent describes the invention of a porous carbon device  which can be useful for electrochemical energy storage devices, thermal dispersion devices, and electric charge dispersion devices. (approved, US Patent#7252884, issued 2007)

1. Li S, Patel S, Hashi C, Huang NF, Kurpinski K. Fabrication of aligned nanofiber scaffolds for vascular and muscular tissue engineering (US Patent Application # US20070269481, filed 2007). This patent application describes the development of vascular and muscular grafts with aligned cellular and matrix structure.  Licensees: NanoVasc and NanoNerve

Published Conference Proceedings

Before 2012

6. Jalil RA, Huang NF, Jame S, Lee J, Nguyen HN, De A, Gambhir S, Reijo-Pera R, Cooke JP. Human Endothelial Cells Derived from Induced Pluripotent Stem Cells Enhance Perfusion in a Murine Model of Peripheral Arterial Disease.  Circulation Supp 122:A20680, 2010

5. Jalil RA, Nguyen HN, Huang NF, Hunter AL, Pera RR, Cooke JP. Human Induced Pluripotent Stem Cell-derived Endothelial Cells Exhibit Heterogeneity. Circulation Supp. 120:S1092, 2009

4. Huang NF, Niiyama H, Peter C, De A, Natkunam Y, Fleissner F, Li Z, Rollins MD, Wu JC, Gambhir SS, Cooke JP. Restoration of Hindlimb Perfusion by Embryonic Stem Cell-Derived Endothelial Cells. Circulation Supp 120: S1152, 2009

3. Huang NF, Lam A, Fang Q, Sievers RE, Li S, Lee RJ.  Injectable Delivery of Mesenchymal Stem Cells in Fibrin for Therapeutic Repair of Chronic Myocardial Infarction.  Circulation Supp 114:II-17, 2006

2. Huang N, Thakar R, Wong M, Kim D, Lee R, Li S. Tissue Engineering of Muscle on Micropatterned Polymer Films. Conf Proc IEEE Eng Med Biol Soc 7:4966-4969, 2004.

1. Luke S, Huang NF, Goyal N, Varkey JA, Alsay-Oculam AA, Gupta M, Ladoulis CT. Optimal Fluorescence in situ Hybridization (FISH) in Cytologic Specimens. Cell Vis 5: 85-86, 1998.

Scientific Conference Posters/Presentations


Huang NF, Lai E, Zaitseva TS, Paukshto MV, Cooke JP. Collagen Nanopatterning Modulates Endothelial Cell Atheroprotective Function, Survival, and Angiogenesis. Basic Cardiovascular Sciences Scientific Sessions, July 14-17, 2014, Las Vegas, NV.

Huang NF, Okogbaa J, Jha A, Lee JC, Zaitseva T, Paukshto MV. Nanopatterned Collagen Scaffolds Promote Blood Perfusion in the Ischemic Limb. Arteriosclerosis Thrombosis and Vascular Biology Meeting Scientific Sessions, May 1-3, 2014, Toronto, Canada. (*winner of Peripheral Vascular Disease Travel Award)

Hou L, Huang NF. Extracellular Matrix-Mediated Differentiation of Human Induced Pluripotent Stem Cells. Experimental Biology, San Diego, CA, April 26-30, 2014

Nakayama K, Rando TA, Huang NF.  Engineering Vascularized Skeletal Muscle with Physiologically-Relevant Cellular Organization. 3rd Annual Symposium on Regenerative Rehabilitation. April 10-11, San Francisco, CA

Hou L, Huang NF. Extracellular matrix enhances generation of human induced pluripotent stem cell-derived endothelial cells for rehabilitative tissue engineering. 3rd Annual Symposium on Regenerative Rehabilitation. April 10-11, San Francisco, CA

Huang NF, Okogbaa J, Jha A, Zaitseva T, Paukshto MV, Cooke JP. Anisotropic Nanofibrillar Collagen Modulates Endothelial Cell Morphology, Function, and Survival. ASME Global Congress on Nanoengineering for Medicine and Biology. February 2-5, 2014, San Francisco, CA2013


Huang NF, Lai E Ribeiro A, Fuller GG, Pruitt B, Cooke JP. Collagen Topographical Patterning Modulates Endothelial Cell Morphology, Gene Expression, And Function. Materials Research Society Spring Conference, April 1-5, 2013, San Francisco, CA

Ostrowski M, Huang EY, Huang NF, Walker TW, Cooke JP, Dun AR, Fuller GG. Upstream migration of endothelial cells in response to impinging fluid flows. Society of Rheology, Quebec, Canada, October 13-17, 2013

Dash R, Kim PJ, Matsuura Y, Ge X, Ikeno F, Lyons JK, Huang NF, Metzler S, Nguyen P, Heidary S, Parent M, Yamamoto T, Cooke J, Ruiz-Lozano P, Robbins RC, Wu JC, McConnell MV, Yeung A, Harnish P, Yang PC. Manganese-Enhanced cardiac MRI (MEMRI) tracks long-term in vivo survival and restorative benefit of transplanted human Amnion-Derived Mesenchymal Stem Cells (hAMSC) after porcine ischemia-reperfusion injury. 16th Annual SCMR Scientific Sessions, February 1-4, 2013, San Francisco, CA

Dash R, Kim PJ, Matsuura Y, Ge X, Ikeno F, Lyons JK, Huang NF, Metzler S, Nguyen P, Heidary S, Parent M, Yamamoto T, Cooke J, Ruiz-Lozano P, Robbins RC, Wu JC, McConnell MV, Yeung A, Harnish P, Yang PC. Sustained Restoration of LV Function in a Porcine Ischemia-Reperfusion Injury Model Using Human Placental Mesenchymal Stem Cells and Manganese-Enhanced MRI.American College of Cardiology, San Francisco, CA, March 9-11, 2013


Huang NF, Ribeiro A, Lai E, Fuller GG, Pruitt B, Cooke JP. Collagen Topographical Patterning Modulates Endothelial Cell Morphology, Gene Expression, And Function. Arteriosclerosis Thrombosis and Vascular Biology Meeting. AHA Scientific Sessions, Chicago, IL, April 18-20, 2012. (*Ngan Huang was winner of Robert W Hobson II Early Career Investigator Award)

Ostrowski MA, Huang NF, Walker T, Khoo S, Devicha M, Poplawski C, Cooke JP, Fuller GG, Dunn AR. Endothelial Cell Response to Complex Flow Profiles. American Society for Cell Biology, San Francisco, December 15-19, 2012