Services at the HTSKC

The Stanford HTSKC offers the following services to ALL Stanford researchers.

  1. Fully automated high-throughput screening (HTS) of Compound Libraries (200,000+ compounds) for both enzyme/protein-based assays and cell-based assays, using the new Thermo Spinnaker Automation System.

  2. Genomic siRNA screening with the siARRAY whole human genome siRNA library from Dharmacon targeting 21,000 genes, using the Agilent Bravo system.

  3. High-Content Screening using the ImageXpress Micro CONFOCAL automated fluorescent microscope with live cell, bright field, and integrated plate handling with the Precise Automation PF400 robot, and image analysis using the MetaXpress software.

  4. High-Throughput Molecular Biology reagents and services, including access to cDNA libraries (Human ORFeome collection, 15,000 genes).

  5. High-throughput assay development assistance with cell culture, experiment design, robotic programming and Standard Operating Procedure drafting.

  6. Screening data analysis assistance with protocols, hit determination and structure activity analyses using Dotmatics Cheminformatics and Genedata Screener.

  7. Use of microplate reader detection systems, including the Tecan Infinite M1000 and Infinite M1000 PRO and BMG CLARIOstar Plus for fluorescence; fluorescence polarization; time-resolved fluorescence; absorbance and luminescence and AlphaScreen); and the Flexstation II 384, for kinetic fluorescence reads to measure calcium mobilization and ion channels.

  8. Use of liquid-handling robots, including the Beckman (Labcyte) Echo 655 acoustic dispenser, Sciclone ALH3000 (96- and 384-well pipetting), Agilent Bravo (96- and 384-well pipetting), Velocity11 VPrep (96-well pipetting), Bio-Tek plate washers/dispensers, Matrix Wellmate and Titertek/Labsystems/Thermo Multidrop and Combi microplate dispensers, BlueCatBio Washer (plate washer), Certus Flex dispenser, and Velocity11 PlateLoc and a4S plate heat sealers.

  9. Training for most of these services and much, much more!

Please contact us for more information.