High-Throughput Molecular Biology at the HTS @ The Nucleus

The HTS @ The Nucleus offers high-throughput molecular biology reagent with its libraries of:

1. cDNA clones from the Human ORFeome Collection (in E. coli stocks), and;

2. The siARRAY whole human genome siRNA library from Dharmacon (of which we are a member of the Genome-Wide RNAi Global Initiative (press release)

3. The Qiagen Mouse Whole Genome siRNA set V1 against 22,124 gene targets.

4. Additional High-Throuhgput Molecular Biology Services include use of 96-well Agilent (formerly Velocity11) VPrep for 96 to 96 and 96 to 384-well plate transfers (VPrep schedule), use of the Agilent Bravo System for PCR Setup and PCR Cleanup applications. (Agilent System schedule), and automation of qPCR on the SFGF's QuantStudio 12K Flex RT PCR.

siRNA Screen Application

Video of our Agilent Robotic System performing 384-well siRNA transfection (schedule).

Videos of our old setup Velocity11 robot performing an RNAi transfection.

Daughter siRNA plate generation

Distribution of siRNA to assay plates (cells are then added for a reverse transfection)

Please contact us if you would like information on either of these high-throughput molecular biology reagents and/or services.

The HTS labs are located in the Sarafan ChEM-H Building Room W008 and CCSR, Room 0133-North Wing, between the Transgenic Research Center and the Stanford Genomics Facility.

For Directions to the HTS labs, see our Contact page.

HTS iLAB Page for HTS registration, room access, and to see equipment Calendars.