Automated Large Object Sorting

The Union Biometrica BioSorter (located in CCSR 0128)

Please contact us for for questions on the BioSorter. 

iLabs BioSorter Schedule (Schedule training here).
View only Calendar for non-registered users

For automated large object sorting and analysis we are utilizing the Union Biometrica BioSorter (datasheetBrochure, User Manual, Quick Reference Guide) integrated to a Thermo Orbitor RS2 Microplate Mover (specsBrochure, User Manual) for sorting to up to 200 microplates.

Please acknowledge the HTBC and NIH SIG S10OD025004 for all publications using the BioSorter.

Union Biometrica Biosorter and Thermo Orbitor

Union Biometric BioSorter

The BioSorter is a multi-range large particle flow cytometer (FACs) for 10-1500 mircon objects capable of sorting to 6-, 12-, 24-, 48-, 96- or 384-well microplate . The BioSorter uses interchangeable Fluidics and Optics Core Assemblies (FOCA) to be able to cover the 10-1500 micron range. The current system has the 250 um FOCA that can sort objects 10-200 microns, but is optimized for 20-150 micron objects such as C. elegans (i.e. worms, nematodes), and large cells (duct cells, hepatocytes, cardiomyocytes, and various stem cells), as well as beads in the 10-200 micron range. The BioSorter uses is then able to analyze the properties of the samples through the FlowPilot III software. Our current BioSorter configuration has both the standard 488nm excitation laser and the 561 nm laser permiting the detection of both GFP, YFP, and mCherry as well as other similar fluorescent proteins or fluorophores.

Automated RT PCR

We have also integrated the SFGF's Applied Biosystems (now part of ThermoQuantStudio 12K Flex real-time PCR system (Schedule, Brochure) to the Thermo Orbitor RS2 to enable automation for such applications as qPCR and Protein Melt high-throughput screens in 384-well format.

To use the BioSorter or the Quantstudio with automation, please contact Dave.

Quantstudio 12K Flex RT PCR with Orbitor

Preferred Sorting Plates

Wells

Vendor

Catalog Number

12

Any

Standard Height

24

Any

Standard Height

48

Any

Standard Height

96

Any with Skirt

Standard Height

384

Any-hardshell with Skirt

Standard Height or PCR

  Lids are OK for all plates  

Technical Information

Description Filter/Laser/Dichroic Slot Wavelength

GFP/Green

Emission

Em1

512/25nm

YFP/Yellow

Emission

Em2

543/22nm

mCherry/Red

Emission

Em3

615/24nm

 

 

 

 

488

Extinction

Ext2

488nm

561

Extinction

 

561nm

1.5 ND

Extinction

Ext1

 

0.6 ND

Extinction

   

1.0 ND

Extinction

   

2.0 ND

Extinction

   

 

 

 

 

488

Laser

488 slot

488 50mW

561

Laser

561 slot

561 50mW

 

 

 

 

526

Dichroic

Di1

526nm

562

Dichroic

Di2

562nm

Mirror

Dichroic

Di3

50%