Selected Publications

Gonzalez VD, Huang YW, Delgado-Gonzalez A, Chen SY, Donoso K, Sachs K, Gentles AJ, Allard GM,

Kolahi KS, Howitt BE, Porpiglia E, Fantl WJ. Cell Reports. 2021.

Key Findings

  • Decidual-like NK cells correlate with tumor cell abundance in tubo-ovarian HGSC
  • Combinatorial NK receptor ligand expression levels differ across tumor compartments
  • NK cells acquire CD9 from HGSC tumor cells via trogocytosis
  • CD9 suppresses anti-tumor cytokine production and cytotoxicity in NK-92 cells

Gonzalez VD*, Samusik N*, Chen TJ, Savig ES, Aghaeepour N, Quigley DA, Huang YW, Giangarra V, Borowsky AD, Hubbard NE,

Chen SY, Han G, Ashworth A, Kipps TJ, Berek JS, Nolan GP, Fantl WJ. Cell Reports. 2018.

Key Findings

  • Cell types identified by CyTOF recur across newly diagnosed HGSOC tumors
  • Greater frequency of vimentin/cMyc/HE4 cells correlates with poor prognosis
  • A transitional EMT phenotype identified co-expressing E-cadherin and vimentin (EV)
  • In relapse patients, EV cells are positively correlated with a metastatic trajectory

Full Publication List

55. Delgado-Gonzalez A, Huang YW, Porpiglia E, Donoso K, Gonzalez VD, Fantl WJ. Measuring trogocytosis between ovarian tumor and natural killer cells. STAR Protocols. 2022 Jun 17;3(2). doi:

54. Lee MY, Bedia JS, Bhate SS, Barlow GL, Phillips D, Fantl WJ, Nolan GP, Schurch CM. CellSeg: a robust, pre-trained nucleus segmentation and pixel quantification software for highly multiplexed fluorescence images. BMC Bioinformatics. 2022 Jan 18;23(1):46. doi: 10.1186/s12859-022-04570-9.

53. Gonzalez VD, Huang YW, Fantl WJ. Mass Cytometry for the Characterization of Individual Cell Types in Ovarian Solid Tumors. Methods Mol Bio. 2022;2424:59-94. doi: 10.1007/978-1-0716-1956-8_4.

52. Gonzalez VD, Huang YW, Delgado-Gonzalez A, Chen SY, Donoso K, Sachs K, Gentles AJ, Allard GM, Kolahi KS, Howitt BE, Porpiglia E, Fantl WJ. High-grade serous ovarian tumor cells modulate NK cell function to create an immune-tolerant microenvironment. Cell Rep. 2021 Aug 31;36(9):109632. doi: 10.1016/j.celrep.2021.109632.

51. Behbehani GK, Finck R, Samusik N, Sridhar K, Fantl WJ, Greenberg PL, Nolan GP. Profiling myelodysplastic syndromes by mass cytometry demonstrates abnormal progenitor cell phenotype and differentiation. Cytometry B Clin Cytom. 2020 Mar;98(2):131-145. doi: 10.1002/cyto.b.21860. 

50. Han G, Spitzer MH, Bendall SC, Fantl WJ, Nolan GP. Metal-isotope-tagged monoclonal antibodies for high-dimensional mass cytometry. Nat Protoc. 2018 Oct;13(10):2121-2148. doi: 10.1038/s41596-018-0016-7. PMID: 30258176; PMCID: PMC7075473.

49. Aghaeepour N, Simonds EF, Knapp DJHF, Bruggner RV, Sachs K, Culos A, Gherardini PF, Samusik N, Fragiadakis GK, Bendall SC, Gaudilliere B, Angst MS, Eaves CJ, Weiss WA, Fantl WJ, Nolan GP. GateFinder: projection-based gating strategy optimization for flow and mass cytometry. Bioinformatics. 2018 Dec 1;34(23):4131-4133. doi: 10.1093/bioinformatics/bty430. PMID: 29850785; PMCID: PMC6247943.

48. Ajami B, Samusik N, Wieghofer P, Ho PP, Crotti A, Bjornson Z, Prinz M, Fantl WJ, Nolan GP, Steinman L. Single-cell mass cytometry reveals distinct populations of brain myeloid cells in mouse neuroinflammation and neurodegeneration models. Nat Neurosci. 2018 Apr;21(4):541-551. doi: 10.1038/s41593-018-0100-x. Epub 2018 Mar 5. PMID: 29507414.

47. Good Z, Sarno J, Jager A, Samusik N, Aghaeepour N, Simonds EF, White L, Lacayo NJ, Fantl WJ, Fazio G, Gaipa G, Biondi A, Tibshirani R, Bendall SC, Nolan GP, Davis KL. Single-cell developmental classification of B cell precursor acute lymphoblastic leukemia at diagnosis reveals predictors of relapse. Nat Med. 2018 May;24(4):474-483. doi: 10.1038/nm.4505. Epub 2018 Mar 5. PMID: 29505032; PMCID: PMC5953207.

46. Gonzalez VD, Samusik N, Chen TJ, Savig ES, Aghaeepour N, Quigley DA, Huang YW, Giangarrà V, Borowsky AD, Hubbard NE, Chen SY, Han G, Ashworth A, Kipps TJ, Berek JS, Nolan GP, Fantl WJ. Commonly Occurring Cell Subsets in High-Grade Serous Ovarian Tumors Identified by Single-Cell Mass Cytometry. Cell Rep. 2018 Feb 13;22(7):1875-1888. doi: 10.1016/j.celrep.2018.01.053. PMID: 29444438.

45. Han G, Chen SY, Gonzalez VD, Zunder ER, Fantl WJ, Nolan GP. Atomic mass tag of bismuth-209 for increasing the immunoassay multiplexing capacity of mass cytometry. Cytometry A. 2017 Dec;91(12):1150-1163. doi: 10.1002/cyto.a.23283. Epub 2017 Dec 4. PMID: 29205767; PMCID: PMC5802970.

44. Saito T, Miyagawa K, Chen SY, Tamosiuniene R, Wang L, Sharpe O, Samayoa E, Harada D, Moonen JAJ, Cao A, Chen PI, Hennigs JK, Gu M, Li CG, Leib RD, Li D, Adams CM, Del Rosario PA, Bill M, Haddad F, Montoya JG, Robinson WH, Fantl WJ, Nolan GP, Zamanian RT, Nicolls MR, Chiu CY, Ariza ME, Rabinovitch M. Upregulation of Human Endogenous Retrovirus-K Is Linked to Immunity and Inflammation in Pulmonary Arterial Hypertension. Circulation. 2017 Nov 14;136(20):1920-1935. doi: 10.1161/CIRCULATIONAHA.117.027589. Epub 2017 Sep 21. PMID: 28935667; PMCID: PMC5685911.

43. Porpiglia E, Samusik N, Ho ATV, Cosgrove BD, Mai T, Davis KL, Jager A, Nolan GP, Bendall SC, Fantl WJ, Blau HM. High-resolution myogenic lineage mapping by single-cell mass cytometry. Nat Cell Biol. 2017 May;19(5):558-567. doi: 10.1038/ncb3507. Epub 2017 Apr 17. Erratum in: Nat Cell Biol. 2018 Mar 5;: PMID: 28414312; PMCID: PMC5728993.

42. Burns TJ, Frei AP, Gherardini PF, Bava FA, Batchelder JE, Yoshiyasu Y, Yu JM, Groziak AR, Kimmey SC, Gonzalez VD, Fantl WJ, Nolan GP. High-throughput precision measurement of subcellular localization in single cells. Cytometry A. 2017 Feb;91(2):180-189. doi: 10.1002/cyto.a.23054. Epub 2017 Jan 17. PMID: 28094900.

41. Spitzer MH, Gherardini PF, Fragiadakis GK, Bhattacharya N, Yuan RT, Hotson AN, Finck R, Carmi Y, Zunder ER, Fantl WJ, Bendall SC, Engleman EG, Nolan GP. IMMUNOLOGY. An interactive reference framework for modeling a dynamic immune system. Science. 2015 Jul 10;349(6244):1259425. doi: 10.1126/science.1259425. PMID: 26160952; PMCID: PMC4537647.

40. Behbehani GK, Samusik N, BjornsMass Cytometry for the Characterization of Individual Cell Types in Ovarian Solid Tumorson ZB, Fantl WJ, Medeiros BC, Nolan GP. Mass Cytometric Functional Profiling of Acute Myeloid Leukemia Defines Cell-Cycle and Immunophenotypic Properties That Correlate with Known Responses to Therapy. Cancer Discov. 2015 Sep;5(9):988-1003. doi: 10.1158/2159-8290.CD-15-0298. Epub 2015 Jun 19. PMID: 26091827; PMCID: PMC4652947.

39. O'Gorman WE, Hsieh EW, Savig ES, Gherardini PF, Hernandez JD, Hansmann L, Balboni IM, Utz PJ, Bendall SC, Fantl WJ, Lewis DB, Nolan GP, Davis MM. Single-cell systems-level analysis of human Toll-like receptor activation defines a chemokine signature in patients with systemic lupus erythematosus. J Allergy Clin Immunol. 2015 Nov;136(5):1326-36. doi: 10.1016/j.jaci.2015.04.008. Epub 2015 May 30. PMID: 26037552; PMCID: PMC4640970.

38. Zunder ER, Finck R, Behbehani GK, Amir el-AD, Krishnaswamy S, Gonzalez VD, Lorang CG, Bjornson Z, Spitzer MH, Bodenmiller B, Fantl WJ, Pe'er D, Nolan GP. Palladium-based mass tag cell barcoding with a doublet-filtering scheme and single-cell deconvolution algorithm. Nat Protoc. 2015 Feb;10(2):316-33. doi: 10.1038/nprot.2015.020. Epub 2015 Jan 22. PMID: 25612231; PMCID: PMC4347881.

37. Behbehani GK, Thom C, Zunder ER, Finck R, Gaudilliere B, Fragiadakis GK, Fantl WJ, Nolan GP. Transient partial permeabilization with saponin enables cellular barcoding prior to surface marker staining. Cytometry A. 2014 Dec;85(12):1011-9. doi: 10.1002/cyto.a.22573. Epub 2014 Oct 1. PMID: 25274027; PMCID: PMC4361015.

36. Gaudillière B, Fragiadakis GK, Bruggner RV, Nicolau M, Finck R, Tingle M, Silva J, Ganio EA, Yeh CG, Maloney WJ, Huddleston JI, Goodman SB, Davis MM, Bendall SC, Fantl WJ, Angst MS, Nolan GP. Clinical recovery from surgery correlates with single-cell immune signatures. Sci Transl Med. 2014 Sep 24;6(255):255ra131. doi: 10.1126/scitranslmed.3009701. PMID: 25253674; PMCID: PMC4334126.

35. Bjornson ZB, Nolan GP, Fantl WJ. Single-cell mass cytometry for analysis of immune system functional states. Curr Opin Immunol. 2013 Aug;25(4):484-94. doi: 10.1016/j.coi.2013.07.004. Epub 2013 Aug 31. PMID: 23999316; PMCID: PMC3835664.

34. Behbehani GK, Bendall SC, Clutter MR, Fantl WJ, Nolan GP. Single-cell mass cytometry adapted to measurements of the cell cycle. Cytometry A. 2012 Jul;81(7):552-66. doi: 10.1002/cyto.a.22075. Epub 2012 Jun 12. PMID: 22693166; PMCID: PMC3667754.

33. Fienberg HG, Simonds EF, Fantl WJ, Nolan GP, Bodenmiller B. A platinum-based covalent viability reagent for single-cell mass cytometry. Cytometry A. 2012 Jun;81(6):467-75. doi: 10.1002/cyto.a.22067. Epub 2012 May 10. PMID: 22577098; PMCID: PMC3808967.

32. Palazzo AL, Evensen E, Huang YW, Cesano A, Nolan GP, Fantl WJ. Association of reactive oxygen species-mediated signal transduction with in vitro apoptosis sensitivity in chronic lymphocytic leukemia B cells. PLoS One. 2011;6(10):e24592. doi: 10.1371/journal.pone.0024592. Epub 2011 Oct 10. PMID: 22016760; PMCID: PMC3189964.

31. Kornblau SM, Covey T, Putta S, Cohen A, Woronicz J, Fantl WJ, Gayko U, Cesano A. Signaling changes in the stem cell factor-AKT-S6 pathway in diagnostic AML samples are associated with disease relapse. Blood Cancer J. 2011 Feb;1(2):e3. doi: 10.1038/bcj.2010.2. Epub 2011 Feb 11. PMID: 22829109; PMCID: PMC3255271.

30. Rosen DB, Minden MD, Kornblau SM, Cohen A, Gayko U, Putta S, Woronicz J, Evensen E, Fantl WJ, Cesano A. Functional characterization of FLT3 receptor signaling deregulation in acute myeloid leukemia by single cell network profiling (SCNP). PLoS One. 2010 Oct 27;5(10):e13543. doi: 10.1371/journal.pone.0013543. PMID: 21048955; PMCID: PMC2965086.

29. Rosen DB, Putta S, Covey T, Huang YW, Nolan GP, Cesano A, Minden MD, Fantl WJ. Distinct patterns of DNA damage response and apoptosis correlate with Jak/Stat and PI3kinase response profiles in human acute myelogenous leukemia. PLoS One. 2010 Aug 25;5(8):e12405. doi: 10.1371/journal.pone.0012405. PMID: 20811632; PMCID: PMC2928279.

28. Kornblau SM, Minden MD, Rosen DB, Putta S, Cohen A, Covey T, Spellmeyer DC, Fantl WJ, Gayko U, Cesano A. Dynamic single-cell network profiles in acute myelogenous leukemia are associated with patient response to standard induction therapy. Clin Cancer Res. 2010 Jul 15;16(14):3721-33. doi: 10.1158/1078-0432.CCR-10-0093. Epub 2010 Jun 4. PMID: 20525753; PMCID: PMC3385931.

27. Easwaran V, Lee SH, Inge L, Guo L, Goldbeck C, Garrett E, Wiesmann M, Garcia PD, Fuller JH, Chan V, Randazzo F, Gundel R, Warren RS, Escobedo J, Aukerman SL, Taylor RN, Fantl WJ. beta-Catenin regulates vascular endothelial growth factor expression in colon cancer. Cancer Res. 2003 Jun 15;63(12):3145-53. PMID: 12810642.

26. Stein M, Bagnoli F, Halenbeck R, Rappuoli R, Fantl WJ, Covacci A. c-Src/Lyn kinases activate Helicobacter pylori CagA through tyrosine phosphorylation of the EPIYA motifs. Mol Microbiol. 2002 Feb;43(4):971-80. doi: 10.1046/j.1365-2958.2002.02781.x. PMID: 11929545.

25. Yan D, Wiesmann M, Rohan M, Chan V, Jefferson AB, Guo L, Sakamoto D, Caothien RH, Fuller JH, Reinhard C, Garcia PD, Randazzo FM, Escobedo J, Fantl WJ, Williams LT. Elevated expression of axin2 and hnkd mRNA provides evidence that Wnt/beta -catenin signaling is activated in human colon tumors. Proc Natl Acad Sci U S A. 2001 Dec 18;98(26):14973-8. doi: 10.1073/pnas.261574498. PMID: 11752446; PMCID: PMC64968.

24. Sun TQ, Lu B, Feng JJ, Reinhard C, Jan YN, Fantl WJ, Williams LT. PAR-1 is a Dishevelled-associated kinase and a positive regulator of Wnt signalling. Nat Cell Biol. 2001 Jul;3(7):628-36. doi: 10.1038/35083016. PMID: 11433294.

23. Yan D, Wallingford JB, Sun TQ, Nelson AM, Sakanaka C, Reinhard C, Harland RM, Fantl WJ, Williams LT. Cell autonomous regulation of multiple Dishevelled-dependent pathways by mammalian Nkd. Proc Natl Acad Sci U S A. 2001 Mar 27;98(7):3802-7. doi: 10.1073/pnas.071041898. PMID: 11274398; PMCID: PMC31133.

22. Haertel-Wiesmann M, Liang Y, Fantl WJ, Williams LT. Regulation of cyclooxygenase-2 and periostin by Wnt-3 in mouse mammary epithelial cells. J Biol Chem. 2000 Oct 13;275(41):32046-51. doi: 10.1074/jbc.M000074200. PMID: 10884377.

21. D'Angelo G, Martini JF, Iiri T, Fantl WJ, Martial J, Weiner RI. 16K human prolactin inhibits vascular endothelial growth factor-induced activation of Ras in capillary endothelial cells. Mol Endocrinol. 1999 May;13(5):692-704. doi: 10.1210/mend.13.5.0280. PMID: 10319320.

20. Yu W, Fantl WJ, Harrowe G, Williams LT. Regulation of the MAP kinase pathway by mammalian Ksr through direct interaction with MEK and ERK. Curr Biol. 1998 Jan 1;8(1):56-64. doi: 10.1016/s0960-9822(98)70020-x. PMID: 9427629.

19. Giese K, Fantl WJ, Vitt C, Stephans JC, Cousens L, Wachowicz M, Williams LT. Reduction of food intake and weight gain by the ob protein requires a specific secondary structure and is reversible. Mol Med. 1996 Jan;2(1):50-8. PMID: 8900534; PMCID: PMC2230025.

18. Hu Q, Klippel A, Muslin AJ, Fantl WJ, Williams LT. Ras-dependent induction of cellular responses by constitutively active phosphatidylinositol-3 kinase. Science. 1995 Apr 7;268(5207):100-2. doi: 10.1126/science.7701328. PMID: 7701328.

17. Ma YH, Reusch HP, Wilson E, Escobedo JA, Fantl WJ, Williams LT, Ives HE. Activation of Na+/H+ exchange by platelet-derived growth factor involves phosphatidylinositol 3'-kinase and phospholipase C gamma. J Biol Chem. 1994 Dec 2;269(48):30734-9. PMID: 7982995.

16. Fantl WJ, Muslin AJ, Kikuchi A, Martin JA, MacNicol AM, Gross RW, Williams LT. Activation of Raf-1 by 14-3-3 proteins. Nature. 1994 Oct 13;371(6498):612-4. doi: 10.1038/371612a0. PMID: 7935795.

15. Baur AS, Sawai ET, Dazin P, Fantl WJ, Cheng-Mayer C, Peterlin BM. HIV-1 Nef leads to inhibition or activation of T cells depending on its intracellular localization. Immunity. 1994 Aug;1(5):373-84. doi: 10.1016/1074-7613(94)90068-x. PMID: 7882168.

14. Timpe LC, Fantl WJ. Modulation of a voltage-activated potassium channel by peptide growth factor receptors. J Neurosci. 1994 Mar;14(3 Pt 1):1195-201. doi: 10.1523/JNEUROSCI.14-03-01195.1994. PMID: 8120619; PMCID: PMC6577584.

13. Satoh T, Fantl WJ, Escobedo JA, Williams LT, Kaziro Y. Platelet-derived growth factor receptor mediates activation of ras through different signaling pathways in different cell types. Mol Cell Biol. 1993 Jun;13(6):3706-13. doi: 10.1128/mcb.13.6.3706-3713.1993. PMID: 8388543; PMCID: PMC359844.

12. Fantl WJ, Johnson DE, Williams LT. Signalling by receptor tyrosine kinases. Annu Rev Biochem. 1993;62:453-81. doi: 10.1146/ PMID: 7688944.

11. Fantl WJ, Escobedo JA, Martin GA, Turck CW, del Rosario M, McCormick F, Williams LT. Distinct phosphotyrosines on a growth factor receptor bind to specific molecules that mediate different signaling pathways. Cell. 1992 May 1;69(3):413-23. doi: 10.1016/0092-8674(92)90444-h. PMID: 1374684.

10. Klippel A, Escobedo JA, Fantl WJ, Williams LT. The C-terminal SH2 domain of p85 accounts for the high affinity and specificity of the binding of phosphatidylinositol 3-kinase to phosphorylated platelet-derived growth factor beta receptor. Mol Cell Biol. 1992 Apr;12(4):1451-9. doi: 10.1128/mcb.12.4.1451-1459.1992. PMID: 1312663; PMCID: PMC369586.

9. Peters KG, Escobedo JA, Fantl WJ, Williams LT. Interactions of PDGF and FGF receptors with cytoplasmic signaling molecules. Cold Spring Harb Symp Quant Biol. 1992;57:63-6. doi: 10.1101/sqb.1992.057.01.008. PMID: 1339700.

8. Williams LT, Escobedo JA, Fantl WJ, Turck CW, Klippel A. Interactions of growth factor receptors with cytoplasmic signaling molecules. Cold Spring Harb Symp Quant Biol. 1991;56:243-50. doi: 10.1101/sqb.1991.056.01.030. PMID: 1668083.

7. Manning LR, Fantl WJ, Manning JM. Functional measurements on carboxymethylated hemoglobin, a potential blood substitute: effects of sodium chloride on pH values that influence the alkaline Bohr effect. Biomater Artif Cells Artif Organs. 1990;18(2):133-41. doi: 10.3109/10731199009117296. PMID: 2369641.

6. Fantl WJ, Escobedo JA, Williams LT. Mutations of the platelet-derived growth factor receptor that cause a loss of ligand-induced conformational change, subtle changes in kinase activity, and impaired ability to stimulate DNA synthesis. Mol Cell Biol. 1989 Oct;9(10):4473-8. doi: 10.1128/mcb.9.10.4473-4478.1989. PMID: 2479827; PMCID: PMC362531.

5. Keating MT, Escobedo JA, Fantl WJ, Williams LT. Ligand activation causes a phosphorylation-dependent change in platelet-derived growth factor receptor conformation. Trans Assoc Am Physicians. 1988;101:24-32. PMID: 2480016.

4. Fantl WJ, Di Donato A, Manning JM, Rogers PH, Arnone A. Specifically carboxymethylated hemoglobin as an analogue of carbamino hemoglobin. Solution and X-ray studies of carboxymethylated hemoglobin and X-ray studies of carbamino hemoglobin. J Biol Chem. 1987 Sep 15;262(26):12700-13. PMID: 3114261.

3. Fantl WJ, Manning LR, Ueno H, Di Donato A, Manning JM. Properties of carboxymethylated cross-linked hemoglobin A. Biochemistry. 1987 Sep 8;26(18):5755-61. doi: 10.1021/bi00392a026. PMID: 3676286.

2. DiDonato A, Fantl WJ, Acharya AS, Manning JM. Selective carboxymethylation of the alpha-amino groups of hemoglobin. Effect on functional properties. J Biol Chem. 1983 Oct 10;258(19):11890-5. PMID: 6619148.

1. Stevens VJ, Fantl WJ, Newman CB, Sims RV, Cerami A, Peterson CM. Acetaldehyde adducts with hemoglobin. J Clin Invest. 1981 Feb;67(2):361-9. doi: 10.1172/JCI110043. PMID: 7462422; PMCID: PMC370576.