Droplet Digital PCR

Leveraging our inhouse Droplet Digital PCR (ddPCR) instrument, we can detect rare mutants. PCR reactions containing fluorescent probes are partitioned into small droplets via microfluidics. This partitioning allows for fluorescent detection of rare targets.

We currently use a ddPCR assay at CCSU to detect histone 3 mutations in cell-free DNA (cfDNA) from patient cerebral spinal fluid (CSF) samples. With our assay, we can detect a rare tumor-associated mutation with high sensitivity (one mutation per ml of CSF). 

For more information, please review our protocol: PMID: 35130560.