Bhutani Lab Publications

  1. Agarwal A*, Lee H-P*, Smeriglio P, Grandi F, Goodman SB, Chaudhuri O* and Bhutani N*. (2021) A dysfunctional TRPV4-GSK3β pathway prevents osteoarthritic chondrocytes from sensing viscoelastic changes in their environment. Nat Biomed Eng. Mar 11, online ahead of print
  2. Sahu N, Agarwal P, Grandi F, Bruschi M, Goodman S, Amanatullah D, Bhutani N. (2021) Encapsulated Mesenchymal Stromal Cell Microbeads Promote Endogenous Regeneration of Osteoarthritic Cartilage Ex Vivo. Adv Healthc Mater. Apr;10(8):e2002118. doi: 10.1002/adhm.202002118. Epub 2021 Jan 12.
  3. Grandi FC and Bhutani N (2021) Mapping 5-hydroxymethylcytosine (5hmC) modifications in skeletal tissues using High-throughput sequencing. Methods Mol Biol 2221: 101-108
  4. Rai MF, Wu CL, Capellini TD, Guilak F, Dicks AR, Muthuirulan P, Grandi F, Bhutani N, Westendorf JJ. (2021) Single Cell Omics for Musculoskeletal Research. Curr Osteoporos Rep. Apr;19(2):131-140
  5. Smeriglio P, Grandi FC, Taylor SEB, Zalc A and Bhutani N (2020) TET1 directs chondrogenic differentiation by regulating Sox9 dependent activation of Col2a1 and Acan in vitro. JBMR plus August 2020; 4(8)
  6. Grandi FC and Bhutani N (2020) Epigenetic therapies for Osteoarthritis. Trends Pharmacol Sci. 2020 Aug;41(8):557-569.

Research highlighted as cover feature

  1. Smeriglio P, Grandi FC, Davala S, Masarapu V, Indelli PF, Goodman SB and Bhutani N (2020) Inhibition of TET1 prevents the development of Osteoarthritis and reveals the 5hmC landscape that orchestrates pathogenesis. Science Translational Medicine, Apr 15; 12(539).

Research highlighted in ‘Nature Reviews for Rheumatology’ and in the NIH newsletter, ‘NIH Research Matters’

  1. Grandi FC, Basker R, Smeriglio P, Mukherjee S, Indelli PF, Amanatullah D, Goodman SB, Chu CR, Bendall S and Bhutani N (2020) Single-cell mass cytometry reveals crosstalk between inflammation dampening and amplifying cells in osteoarthritic cartilage. Science Advances, Mar 13;6(11)
  2. Sarkar TJ, Quarta M, Mukherjee S, Colville A, Paine P, Doan L, Tran CM, Chu CR, Horvath S, Qi LS, Bhutani N, Rando TA, Sebastiano V (2020) Transient non-integrative expression of nuclear reprogramming factors promotes multifaceted amelioration of aging in human cells. Nature Communications Mar 24;11(1):1545.
  3. Bruschi M, Agarwal P & Bhutani N. (2020) Induced Pluripotent Stem Cells-derived Chondrocyte Progenitors. Book Chapter- Induced Pluripotent Stem Cells – Novel Concepts (Springer)
  4. O’Donnell C., Migleori E, Grandi F., Koltsov J., Lingampalli N, Indelli P, Sebastiano V, Robinson WH, Bhutani N and Chu CR (2019) Platelet-Rich Plasma (PRP) from Older Males with Knee Osteoarthritis Depresses Chondrocyte Metabolism and upregulates Inflammation. J Orthop Res. Aug;37(8):1760-1770.
  5. Adkar SS, Wu C, Willard VP, Dicks A, Ettyreddy A, Steward N, Bhutani N, Gersbach CA and Guilak F. Highly efficient chondrogenic differentiation of human iPSCs and purification via a reporter allele generated by CRISPR-Cas9 genome editing (2019)  Stem Cells. Jan 37(1), 65-76
  6. Jake A. Mooney, Piera Smeriglio, Nidhi Bhutani and Derek F. Amanatullah. Effect of Trabecular Metal on the Elution of Gentamicin from Palacos Cement. J Orthop Res. 2019 May; 37(5):1018-1024. doi: 10.1002/jor.24274.
  7. Optimizing Clinical Use of Biologics in Orthopedic Surgery: Consensus Recommendations From the 2018 AAOS/NIH U-13 Conference. Chu CR, Rodeo S, Bhutani N, Goodrich LR, Huard J, Irrgang J, LaPrade RF, Lattermann C, Lu Y, Mandelbaum B, Mao J, McIntyre L, Mishra A, Muschler GF, Piuzzi NS, Potter H, Spindler K, Tokish JM, Tuan R, Zaslav K, Maloney W. J Am Acad Orthop Surg. 2019 Jan 15;27(2)
  8. Xiong G, Lingampalli N, Koltsov JCB, Leung LL, Bhutani N, Robinson WH & Chu CR. (2018) Males and Females Differ in Biochemical Composition of Platelet-rich Plasma. American Journal of Sports Medicine Feb 46(2), 409-410
  9. Lee J, Smeriglio P, Chu CR, Bhutani N. (2017) Human iPSC-derived chondrocytes mimic juvenile chondrocyte function for the dual advantage of increased proliferation and resistance to IL-1beta. Stem Cell Res Ther. Nov 2;8(1):244.
  10. Smeriglio P, Lee J & Bhutani N (2017) Soluble Collagen VI Treatment enhances Mesenchymal Stem Cells Expansion for Engineering Cartilage. Bioengineering and Translational Medicine Sept 2017, 2(3), 278-284.
  11. Meulenbelt IM, Bhutani N, den Hollander W, Gay S, Oppermann U, Reynard LN, Skelton AJ, Young  DA, Beier F, Loughlin J. (2017) The first International Workshop on the Epigenetics of Osteoarthritis. Connect Tissue Res. Jan; 58(1):37-48.
  12. Lee J., Smeriglio P., Dragoo J., Maloney W.J. & Bhutani N. (2016) CD24 enrichment protects while its loss increases susceptibility of juvenile chondrocytes towards inflammation. Arthritis Res & Therapy 18(1), 292.
  13. Taylor SE, Lee J, Smeriglio P, Razzaque A, Smith RL, Dragoo JL, Maloney WJ, Bhutani N. (2016) Identification of human Juvenile chondrocyte-specific factors that stimulate stem cell growth. Tissue Eng. Part A. Apr; 22 (7-8):645-53
  14. Taylor SE, Li YH, Smeriglio P, Rath M, Wong WH, Bhutani N (2016) Stable 5hmC acquisition marks gene activation during chondrogenic differentiation. J Bone Min Res Mar:31(3):524-34
  15. Smeriglio P, Lai JH, Yang F, Bhutani N. (2015) 3D Biomimetic Hydrogels for Articular Chondrocyte Culture and Cartilage generation. Journal of Vis Exp (JoVE) Oct 7 (104)
  16. Taylor S., Li Y.L., Wong W.H. & Bhutani N. (2015) Genome-wide mapping of DNA hydroxymethylation in osteoarthritic chondrocytes. Arthritis Rheumatol. 67(8), 2129-40.
  17. Lee J., Taylor S.E.B., Smeriglio P., Lai J., Maloney W.J., Yang F. & Bhutani N. (2015) Early induction of a pre-chondrogenic population allows efficient generation of stable chondrocytes from human induced pluripotent stem cells. FASEB J Aug; 29 (8): 3399-410.
  18. Smeriglio P, Dhulipala L, Lai JH, Goodman SB, Dragoo JL, Smith RL, Maloney WJ, Yang F, Bhutani N. (2015) Collagen VI enhances cartilage tissue generation by stimulating chondrocyte proliferation. Tiss. Eng. Part A Feb;21(3-4):840-9.
  19. Smeriglio P., Lai J.H., Dhulipala L., Behn A.W., Goodman S.B., Smith R.L., Maloney W.J., Yang F.* & Bhutani N.* (2015) Comparative Potential of Juvenile and Adult Human Articular Chondrocytes for Cartilage Tissue Formation in 3D Biomimetic Hydrogels. Tiss. Eng. Part A Jan;21(1-2):147-55.
  20. Taylor S., Smeriglio P., Rath M., Dhulipala S. & Bhutani N. (2014) A global increase in 5-hydroxymethylcytosine levels marks Osteoarthritic chondrocytes. Arthritis Rheumatol. 66(1), 90-100
  21. Bhutani N.*, Decker M.N.*, Brady J.J., Bussat R.T., Burns D.M., Corbel S.Y. & Blau H.M. (2013) A critical role for AID in the initiation of reprogramming to induced pluripotent stem cells. FASEB J., 27(3), 1107-13.
  22. Bhutani N.*, Piccirillo R.*, Hourez R., Venkatraman P. & Goldberg A.L. (2012) Cathepsins L and Z are critical in degrading polyglutamine-containing proteins within lysosomes. J Biol. Chem., 287(21), 17471-82
  23. Bhutani N., Burns D.M. & Blau H.M. (2011) DNA demethylation dynamics. Cell, 146(6), 866-72
  24. Bhutani N.*, Brady J.*, Damian M.R., Sacco A., Corbel, S.Y. & Blau H.M. (2010) Reprogramming towards pluripotency requires AID-dependent DNA demethylation. Nature, 463(7284), 1042-7
  25. Palermo A., Doyonnas R., Bhutani N., Alkan O., Pomerantz J., Blau H.M. (2009) Nuclear reprogramming in heterokaryons is rapid, extensive and bidirectional. FASEB J 23(5), 1431-40
  26. Bhutani N., Venkatraman P., Goldberg A.L. (2007) Puromycin-sensitive aminopeptidase is the major cytosolic protease responsible for degrading poly-glutamine peptides released by the proteasome. EMBO J  26(5), 1385-96
  27. York I.A., Bhutani N., Zendcien S., Goldberg A.L., Rock K.L. (2006) Tripeptidyl peptidase II (TPPII) trims long antigenic precursors, but is not required for most MHC Class I antigen presentation. J.Immunology 177(3), 1434-43
  28. Chang S.C, Momberg F., Bhutani N., Goldberg A.L. (2005) A molecular ruler mechanism for the length preference of ERAP1, a key enzyme in antigen presentation. Proc. Natl. Acad. Sci. 102(47), 17107-12.
  29. Bhutani N., Udgaonkar J.B. (2003) Folding sub-domains of thioredoxin characterized by Native state hydrogen-exchange. Protein Sci. 12 (8), 1719-31.
  30. Bhutani N., Udgaonkar J.B. (2002) Chaperonins as protein folding machines. Curr. Sci. 83 (11), 1337-1351.
  31. Bhutani N., Udgaonkar J.B. (2001) GroEL channels the folding of thioredoxin along one kinetic route. J. Mol. Biol. 314 (5), 1167-1179.
  32. Bhutani N., Udgaonkar J.B. (2000) A thermodynamic coupling mechanism can explain the GroEL-mediated acceleration of the folding of barstar. J. Mol. Biol. 297(5), 1037-1044.
  33. Bhutani N., Ranjith M.R., Yameen M., Singh N., Dev V., Pillai C.R., Ansari M.A., Sharma Y.D., (1998) Genetic diversity among fields isolates of P. falciparum in India. Curr. Sci. 75, 160-163.
  34. Sharma YD, Fakruddin JM, Bhutani N, Kaushik R, Raina OK & Sharma I. (1997) Strain variation and gene hunting in Malaria. Indian J Clin. Biochem. 12(Suppl 1):49-51.

* Equal authors or co-corresponding authors


  1. US2017/0312316A1 Cartilage cells with a high regenerative potential and low immune response for use in cellular therapy. Nidhi Bhutani, Jieun Lee and Sarah Taylor.
  2. US20/54004 Methods and compositions for the treatment of Osteoarthritis. Nidhi Bhutani and Fiorella Grandi