Cell Sciences Imaging Facility (CSIF)
About CSIF
The Cell Sciences Imaging Facility (CSIF) provides state-of-the-art technologies for quantitative imaging based analysis of biological systems that span bioengineered materials to organisms. The facility offers sophisticated and demanding microscopy techniques, supported by highly trained research scientists to facilitate science across all of Stanford University and beyond.
The CSIF is organized into three integrated imaging groups: the Optical Microscopy Group (OMG) provides in depth training and assistance in experimental design across cutting-edge microscopy techniques and image analysis software packages. The Electron Microscopy Group (EMG) provides expertise in biological sample preparation and imaging for high-resolution scanning and transmission electron microscopes. The Spatial Multiplexing Group (SMG) provides consultation, imaging and analysis services for highly multiplexed protein and RNA detection in biological tissue samples. These three groups are distributed across four Stanford University sites, each integrated and configured for their local communities: CSIF Beckman in the School of Medicine (SoM), CSIF Shriram in the School of Engineering (SoE), CSIF Neuroscience Microscopy Service in the Wu Tsai Neurosciences Institute and a satellite small facility in the Lorey Lokey Stem Cell Building. The CSIF is also a participating member of the Stanford Cancer Institute, a National Cancer Institute-designated Comprehensive Cancer Center.
CSIF Staff: Left to Right: Kitty Lee, Yuanyuan Li, Marika Cai, Gordon Wang, Nadia Makarova, Yili Zhu, John Perrino, David Lenzi.
Services
Light Microscopy
- Confocal microscopy (scanning and spinning-disk)
- Two-photon microscopy
- Wide-field fluorescence microscopy
- Digital deconvolution
- Transmitted-light imaging (phase, DIC, histology)
- High-content screening (confocal and wide-field)
- Super-resolution imaging (SIM and AiryScan)
- Cell surface imaging with <100 nM z-resolution (TIRF)
- Specialized microscopy (FRAP, FRET, FLIP, etc.)
- Fluorescence lifetime imaging microscopy (FLIM)
- Lattice light-sheet microscopy (LLSM)
- Light-sheet microscopy (LSM)
Spatialomics Imaging
- Array tomography (Super-resolved, 8-25 protein markers)
- Akoya CODEX system (whole slide, 50+ protein markers)
- Stellaromics Pyxa imaging system (250+ RNA transcripts)
Atomic Force Microscopy
- Bruker BioScope Resolve
- BrukerNanoWizard 5
Electron Microscopy
- Chemical and cryo-fixation/freeze substitution processing
- High-pressure freezing (w/ opto and electric stimulation)
- Immuno-EM
- Correlative LM to EM: CLEM
- Negative staining
- Ultramicrotomy, cryo and plastic
- Electron tomography (plastic sections)
- High-resolution FE-SEM
- Immuno-SEM
- Array tomography SEM, CLEM
Bioimage Analysis
- Provide software for image analysis, processing, and deconvolution (Imaris, Volocity, SVI Huygens, Neurolucida, SoftWoRx, Microvolution)
Jon Mulholland
Co-Director, CSIF
Beckman/Shriram/Wu Tsai-NMS
jwm@stanford.edu
Gordon Wang, PhD
Co-Director, CSIF
Beckman/Shriram/Wu Tsai-NMS
drwonder@stanford.edu