If samples are submitted ASAP to our receiving/processing labs, we will perform the centrifugation to remove serum or plasma. Do NOT centrifuge any sample for hematology. If samples need to be stored prior to submission for chemistry or serology, you should centrifuge them, remove the serum/plasma and store it appropriately (see below).
We strongly recommend turning in samples by 3 pm for same day processing. Hematology and some chemistry samples require same-day processing. If your sample is submitted after 3:30 p.m., the integrity of the test result may be compromised due to the inability to test immediately.
For hematology (whole blood), refrigerate samples at 4°C if they are not immediately brought to our lab. Please submit the samples as soon as possible and never put them on ice or in a freezer. For chemistry and serology samples, please bring to the lab ASAP because the centrifugation step should be performed immediately. If samples cannot be submitted the same day, you will need to centrifuge samples and remove the serum/ plasma. This can then be stored either overnight at 4°C or in a freezer for long-term storage. For microbiology and PCR samples, sample handling depends on the specific test requested. In general, microbiology culture samples should be refrigerated at 4°C if they are not immediately brought to our lab.
We send out cell line samples to a specific reference lab every Monday. If you submit your cell line by 3 pm on Monday, we can include your sample with our regular shipment, and you will not be billed for a shipping charge. You can also submit your sample prior to the day of shipping since we freeze these samples at -20 until shipping.
In short, cell lines should be submitted in a cryovial in the original culture media. Do not pellet the cells. Submit between 2 x 106 and 10 x 106 cells in < 1 mL of media. Do NOT submit greater than 10 x 106 cells. We encourage users to pool up to 5 separate cell lines in a single cryovial for submission. The guidelines for pooled samples are the same: submit between 2 x 106 and 10 x 106 total cells in < 1 mL of total media. If a pooled sample has a positive test result for an agent, each individual cell line will then be tested for that one agent – this is more efficient and cost-effective than testing each individual cell line for all agents.
For submission of non-cellular biological specimens (e.g. serum, ascites, virus, parasite stock, or other liquid), submit one cryovial containing 0.2 - 0.5 ml of undiluted fluid.
For advice about blood collection techniques, please contact Greg Nelson (Veterinary Tech Supervisor); phone (650) 498-6729.