Samples for hematology alone should be submitted in a lavender-top tube (contains the anticoagulant EDTA).
Most chemistry tests can be run on either 1) serum obtained by collecting blood into a regular (empty) plastic tube – blood is centrifuged after clotting and serum is drawn off into new plastic tube; or 2) plasma obtained by collecting blood into a green-top tube (contains the anticoagulant heparin) – blood is centrifuged and plasma is drawn off into a new plastic tube. If both hematology and chemistry are run on a single sample, blood should be drawn into a green-top heparin tube and immediately submitted WITHOUT centrifugation.
Serology samples require serum obtained as described above.
- All chemistry testing requires a single “dead” volume in addition to the specific volumes required to perform each of the tests. The Diagnostic Lab has a volume chart available online on our website: Blood volume testing handout (this form has been updated). You may also pick up a copy from either of our submission labs. If you are submitting a mouse sample, we recommend that you list your test priorities on your request form to help us prioritize if sample volume is not sufficient to run all tests.
Calculating the exact volume required for multiple tests can be complicated. We are able to maximize the usefulness of a small sample by diluting some tests without adverse effects, but this ability is entirely test-dependent. Please contact us for further help: Roberta Moorhead (lab manager); phone (650) 723-3878.
Some considerations for preanalytical dilutions in hematology and biochemistry analysis can be found in the following articles:
Alterations due to dilution and anticoagulant effects in hematologic analysis of rodent blood samples on the Sysmex XT-200iV
Bias due to Preanalytical Dilution of Rodent Serum for Biochemical Analysis on the Siemens Dimension Xpand Plus
Because of the small volume obtainable from a survival mouse bleed, it is very difficult to collect enough blood to run both a complete chemistry panel and a CBC. A single lavender-top (EDTA) microtainer tube should be used to collect the sample for CBC. For the chemistry panel placing whole blood in a plain Eppendorf will suffice. We have these tubes available in the lab for your use. Please see the following guidelines for blood collection in rodents: APLAC Guidelines for Blood Collection. As above, please list test priorities on the submission form to help us prioritize if sample volume is not sufficient to run all tests.
Please contact Greg Nelson (Veterinary Tech Supervisor); phone (650) 498-6729.
If you are submitting the samples to our lab for testing, we have all collection tube types available in our Edwards Building Room R320 receiving lab for you to use, free of charge.
Even if a test is not listed on our request form, we may be able to perform the test. The request form lists our most common tests. For more information, please contact Roberta Moorhead (lab manager); phone (650) 723-3878.
We request that you contact the Diagnostic Lab in advance if you are submitting a large quantity of samples. The advance notice helps to ensure the efficient processing of your samples. Also, it can sometimes be financially advantageous to submit samples in batches, so please contact us for more information about a potential cost break.
You can obtain a price list at the Diagnostic Lab drop-off sites at Edwards Building Room R320 and CMP, or view the Diagnostic Laboratory Rates online.