Stanford University Stanford Hospital & Clinics Lucile Packard Children's Hospital VA Palo Alto Santa Clara Valley Medical
Stanford School of Medicine Transgenic Research Center in the Department of Pathology
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transgenic research center

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> Injection Schedule
 

Sperm freeze & ICSI

Intracytoplamic sperm injection (ICSI) for generating mouse embryos from cryopreserved spermatozoe

Scope of service:
We will cryopreserve sperms from two healthy males of the line you requested, store the sperms in our liquid nitrogen freezer, and give you equal vials of sperms to keep in your own location. When you need to use the lines again, we will perform intracytoplasmic sperm injection and generate animals for you.

Fees:
The fee for sperm cryopreservation and storage is $750 per line. We will store up to 5 vials of sperms in our liquid nitrogen freezer for you.
The fee for ICSI is $3,200. We will perform multiple rounds of sperm injections to generate live embryos or pups. This fee also covers all the animal cost involved in the experiment.