ES cell manipulation services for gene targeting experiments
We accept linearized targeting vector DNA, purified and resuspended in
50 ul of PBS or TE buffer in the amount of 50 ug. We ask that users
submit a restriction map of the targeting vector and a picture of the
gel showing the purified DNA with a DNA mass standard for quantitation.
We will perform electroporation, selection and colony picking. We will
provide all of the clones resulted from the electroporation if less
than 100 clones are obrtained, or a minimum of 100 ES clones to users
to screen for homologous recombinants. If more clones are necessary,
we will perform a second round of electroporation for free and provide
the users with additional clones for screening. After the ES cells
are handed to users for screening, we expect to get genotyping results
from the users within 6 months; otherwise the cells will be discarded.
The facility currently has CGR8.8 (129ola) and R1 (129sv/svj) cell
lines for gene targeting experiments.
Additional fees for re-expansion: If additional ES clone expansion is
requested due to special needs of the user, a fee of $150/vial will
be charged.
