In vivo tumor models
Xenograft and orthotopic models
Appropriate number of cells will be prepared into single cell suspension in 50-100μL volume (PBS:matrigel=1:1) and injected subcutaneously on the flank or the abdominal area of the mice.
Mammary fat pad model
A midline incision will be made through skin and fascia. Mammary fat pad will be identified and breast carcinoma cells will be injected at the concentration of 1-5X106 in 30μL PBS or with 50% matrigel. Wounds will be sealed with suture or would clip.
Cells will be labeled with luciferase or other reporter genes. Appropriate number of cells will be prepared into single cell suspension in <50μL of PBS. Cells will be injected through tail vein or retro-orbitally.
Cells are injected into the kidney capsule for teratoma formation. Incision will be made on the back near the kidney sites. About 20-50μL of ~2X106 cells will be injected into the kidney capsule. Muscle incision is sealed with absorbable suture and skin is sealed with wound clip or non-absorbable suture.