Automated Multiplex Oligonucleotide Synthesizer version II (AMOSvII) Indented Parts List v.21 (8/20/98) Note: The designation "NE" following assembly drawing call outs indicates that the drawing is nonexistent, but has been assigned a title and number for the purpose of Parts List organization. 100004 Rev. 10 Fluid and Gas Routing Assembly 100005 Rev. 4 Gas Plumbing Layout 110000A R0 Frame Assembly, Front View 110000B R0 Frame Assembly, Rear View 110000C R0 Frame Assembly, Side Views 110101 R2 Base Plate 110102 R7 L1 Rails (Bearing Mount Detail) 110102 R5 L2 Rails (Mounting Hole Detail) 110103 R1 Rail Posts 110104 R3 L1 Front Shelf Post 110104 R0 L2 Rear Shelf Post 110105 R0 Shelf Post Spacer 110108 R0 Linear Drive 110109 R2 Linear Actuator Linkage 110111 R1 Rail Hinge 110112 R1 Upper Side Spacer Bracket 110113 R1 Upper Liquid Valve Bracket 110114 R0 Lower Liquid Valve Bracket 110115 R2 Left Lower Side Spacer Bracket 110118 R3 Diverter Valve Bracket 110119 R0 Bearing Axle 110120 R0 Ball Bearing for Moving Plate 110124 R1 Upper PC Card Support 110125 R2 Lower PC Card Support 110129 R2 Right Lower Side Spacer Bracket 110130 R3 Linear Drive Mounting Bracket (For Model R3S23N-105B-30-I-ASE) 110131 R0 Reagent Shelf Assembly 110132 R3 Reagent Shelf 110133 R4 L1 Reagent Shelf Bracket 110133 R1 L2 Reagent Shelf Bracket Valve and Cable Mounting Hole Diagram 110134 R0 Hinge Pin 110135 R0 Large TCA and DCM Support Plate 110200 R0 Moving Plate Assembly 110201 R1 Moving Plate 110202 Purge Manifold Assembly (NE) 110204 R3 L1 Purge Manifold Block 110204 R3 L2 Purge Manifold, Step Detail 110205 R1 Clamp for 96 Well Plate 110206 R0 Shoulder Screw 110207 R2 Purge Manifold Window 110208 R0 Purge Manifod Retainer Plate 110209 R2 Glass Blank, Window, Purge Manifold Note: The source of nuts, ferrules, etc., designated by a P-XXX type number are products of Upchurch Scientific, Oak Harbor, WA 1 ea P-132 Nut 1 ea P-133 Ferrule 1 ea 1/8 NPT elbow to 1/4" OD tubing 1 ea tubing 3/16"OD x .125"ID x 8' FEP tubing 1 ea tubing 1/4"OD x 3/16"ID x 8' polypropylene tubing 110210 R0 Reaction Chamber Assembly 110211 R1 Bottom Chamber 110212 R0 Chamber Drain Plate 110213 R3 Rear Window Frame 110214 R3 Rear Window Retainer Note: 110213 and 110214 are alternate parts for 110226 when a view through the Reaction Chamber is desired. A backlight can be provided by part nos. 116007 and 116008. 110215 R3 Chamber Window 110216 R6 Front Window Frame 110217 R3 Front Window Retainer 110219 R2 Right Latch Plate for Window 110220 R3 Left Latch Plate for Window 110221 R2 Window Fastener Mount 110222 R2 Top Chamber 110223 R2 Moving Plate Detail: Purge Manifold and Plate Linkage Hole Location 110224 R0 Seat for 96 Well Plate 110225 R1 Window Hinge 110226 R2 Rear Chamber Wall 110227 R0 L1 Alt. 96 Well Plate Seat 110228 R0 Bottom Chamber Detail 110229 R0 Trityl Spacer 110232 R0 Oros Technologies 96 Well Plate 110230 R2 Plate Linkage 110231 R0 Flexible Link 110300 R0 Top Plate Assembly 110301 R1 Top Plate 110302 R0 Top Plate Seal Ring 110303 R0 Top Plate Hinge 110304 R0 Top Plate Window Frame 110305 R0 Top Plate Window 110306 R0 L1 Top Plate Teflon Gasket Seal 110306 R0 L2 Top Plate Teflon Gasket Template 110307 R1 L1 Top Plate Rubber Gasket 110307 R0 L2 Top Plate Rubber Gasket Template 111000 Gas Manifold Assemblies (NE) 111002 R0 Gas Inlet/Outlet Manifold 111003 R1 Argon Manifold, Type 1 1 ea B-500-1-4 Swagelok fitting (5/16"x3/16"tubing to 1/4" NPT female) 7 ea P-304x nut 7 ea P-300nx ferrule 111004 R1 Argon Manifold, Type 2 7 ea P-304x nut 7 ea P-300nx ferrule 111005 R1 Input Regulator Mounting Plate 112000 Liquid Manifold Assemblies (NE) 112001 R0 Nozzle Block 112002 R0 Tet and TCA Nozzle Block 112003 R0 Tet and TCA Nozzle Wash Plate 112004 R2 Outer Wash Tubes for Wash Plate 112200 R5 Reagent Valve Manifold 112300 R2 ACN Manifold, Type 1 2 ea P-132 nut 2 ea P-133 ferrule 6 ea P-304x nut 6 ea P-300nx ferrule 112301 R2 ACN Manifold, Type 2 1 ea P-132 nut 1 ea P-133 ferrule 1 ea P-311 plug 5 ea P-304x nut 5 ea P-300nx ferrule 112302 R1 ACN Tee 2 ea P-132 nut 2 ea P-133 ferrule 1 ea P-204x nut 1 ea P-200nx ferrule 113000 Process Reagent Supply Components (NE) 113001 Reagent Supply Assemblies (Type 1)(6 per system) (NE) 1 ea 113006 Tapered Reagent Bottle 1 ea 113009, type 1 Valve Cap 2 ea P-304 nut 2 ea P-300nx ferrule 3ea 113008 Valve Handle 113002 Reagent Supply Assemblies (Type 2)(4 per system) (NE) 1 ea VWR 250 ml Reagent Bottle 1 ea 113009, type 1 Valve Cap 2 ea P-304 nut 2 ea P-300nx ferrule 1 ea P-204x nut 1 ea P-200nx ferrule 3ea 113008 Valve Handle 1 ea LFVA1210320H Valve (vent) 113003 Reagent Supply Assembly (Type 3)(1 per system) (NE) 1 ea VWR 1000 ml Reagent Bottle (alternate: VWR 10000 ml Reagent Bottle) 1 ea 113009, type 1 Valve Cap 2 ea P-304 nut 2 ea P-300nx ferrule 1 ea P-204x nut 1 ea P-200nx ferrule 3ea 113008 Valve Handle 1 ea LFVA1210320H Valve (vent) 113004 Reagent Supply Assembly (Type 4)(1 per system) (NE) 1 ea VWR 2000 ml Reagent Bottle 1 ea 113009, type 1 Valve Cap 2 ea P-330 nut (PEEK) 2 ea P-300nx ferrule 1 ea P-230 nut (PEEK) 1 ea P-200nx ferrule 3ea 113008 Valve Handle 1 ea LFVA1210320H Valve (vent) 113005 Reagent Supply Assembly (Type 5)(1 per system) (NE) 1 ea VWR 10000 ml Reagent Bottle 1 ea 113009, type 2 Valve Cap 1 ea P-132 nut 1 ea P-133 ferrule 1 ea P-304x nut 1 ea P-300nx ferrule 1 ea P-204x nut 1 ea P-200nx ferrule 3 ea 113008 Valve Handle 1 ea LFVA1210320H Valve (vent) 113006 R1 Tapered Reagent Bottle 113007 R0 Bottle Removal Tool 113008 R0 Rainin Cap Valve Handle 113009 R0 Modified 45-3282 Rainin Valve Cap 114000 Automated Valve Assemblies (NE) 114100 TCA Delivery Assembly (1 per system) 114101 Components List, TCA 9 ea LFVA1210320H Valve 1 ea LFYA1215010H Valve 1 ea 114102 Valve Stack Spacer 16 ea 114701 062 Minstac Assemblies 3 ea 114702 125 Minstac Assemblies 1 ea 112200 Reagent Valve Manifold 8 ea P-230 nut (PEEK) 8 ea P-200nx ferrule 1 ea P-330 nut (PEEK) 1 ea P-300nx ferrule 1 ea P-311 plug 2 ea 114103 Valve Support Rods 1 ea 114600 Valve Board and Terminal Block 114102 R0 Valve Stack Spacer 114103 R0 Valve Support Rods 114600 R0 Valve Board and Terminal Block 114200 Tetrazole Delivery Assembly (1 per system) 114201 Components List, Tet. 9 ea LFVA1210320H Valve 1 ea LFYA1215010H Valve 1 ea 114102 Valve Stack Spacer 16 ea 114701 062 Minstac Assemblies 3 ea 114702 125 Minstac Assemblies 1 ea 112302 R0 ACN Tee 1 ea 112200 R5 Reagent Valve Manifold 8 ea P-204x nut 8 ea P200nx ferrule 1 ea P-304x nut 1 ea P-300nx ferrule 1 ea P-311 plug 2 ea 114102 Valve Support Rods 1 ea 114600 Valve Board and Terminal Block 114300 General Reagent Delivery Assembly (9 per system) 114301 Components List, General Reagent 8 ea LFVA1210320H Valve 1 ea LFYA1215010H Valve 1 ea 114102 Valve Stack Spacer 16 ea 114701 062 Minstac Assemblies 3 ea 114702 125 Minstac Assemblies 1 ea 112200 R5 Reagent Valve Manifold 8 ea P-204x nut 8 ea P200nx ferrule 1 ea P-304x nut 1 ea P-300nx ferrule 1 ea P-311 plug 2 ea 114102 Valve Support Rods 1 ea 114600 Valve Board and Terminal Block 114400 ACN Delivery System (1 per system) 114401 Components List, ACN 8 ea LFVA1210320H Valve 1 ea 114102 Valve Stack Spacer 16 ea 114701 062 Minstac Assemblies 1 ea 112200 R5 Reagent Valve Manifold 8 ea P-204x nut 8 ea P200nx ferrule 1 ea P-304x nut 1 ea P-300nx ferrule 1 ea P-311 plug 2 ea 114102 Valve Support Rods 1 ea 114600 Valve Board and Terminal Block 114500 DCM Delivery System (1 per system) 114501 Components List 1 ea LFVA1210320H valve 2 ea 114701 062 Minstac Assembly 1 ea 114702 125 Minstac Assembly 1 ea Upchurch P-716 Tee 2 ea P-330 nut (PEEK) 2 ea P-300nx ferrule 1 ea P-204x nut 1 ea P200nx ferrule 114600 Valve Board and Terminal Block 114700 Minstac Connector Assemblies (NE) 114701 062 Minstac Connector Assemblies 1 ea TMAA3202079Z Coupling Screw 1 ea TMCA3202030Z Collet Sleeve 1 ea TMBA3202910Z Ferrule Assembly Tools TTTA3201543A Chamfer Ferrule Tool TTTA3201443A Collet Tool TTTA3201243A Torque Wrench TTTA3200543C Spline Wrench 114702 125 Minstac Connector Assemblies 1 ea TMAA6201921Z Coupling Screw 1 ea TMCA6201920Z Collet Sleeve 1 ea TMBA6201910Z Ferrule Assembly Tools TTTA6200143A Coupling Assembly Tool TTTA6202227A Torque Wrench TTTA6200227C Wrench 114800 Gas Flow Controls (NE) 114801 Top Plate System (NE) 3 ea LFDX0500200A valves (normally closed) 1 ea FM 4491 Flowmeter (Advanced Specialty Gas Equipment) 1 ea Victor HPL 161A Pressure Regulator with gauge 114802 Reagent Supply System (NE) 1 ea 114803 R0 Buzmatics SPCJR Electronic Pressure Regulator 115000 Miscellaneous Mechanical Assemblies (NE) 115001 R1 Motor Controller Baseplate 115003 R0 Valve Power Supply Baseplate for SE-150-2 115004 R0 Screw Storage Block 115005 R0 Filter Punch and Insertion Tool Assembly (NE) 115006 Filter Assembly Tool, Layout Dwg. 115007 R0 Punch 115008 R0 Ejector Rod 115009 R0 Punch Holder Plate 115010 R0 Rod Holder Plate 115011 R0 Base Plate 115012 R1 Sliding Plate 115013 R0 Back Hinge 115014 R0 Middle Hinge 115015 R0 Lever Arm 115016 R1 Support Box 115017 R0 Hold Down Bar 115018 R0 Top Plate Holdup Bar 116000 Miscellaneous Electrical Assemblies (NE) 116001 R2 L1 Connector Case for 40 Conductor Ribbon Cable, Plastic Cover 116001 R2 L2 Connector Case for 40 Conductor Ribbon Cable, Plastic Bottom 116002 R0 Connector Diagram for Ribbon Cable Socket for SSR DC Source 116003 R1 Connector Case Mounting Base 116004 R5 Connector Diagram SX-6 Drive for Linear Drive Actuator 116005 R0 Flat Ribbon Cable No. 13 Pinout Table 116006 R3 Flat Ribbon Cable No. 14 Pinout Table 116007 R0 Backlight Support 116008 R0 Backlight (Special Phillips Linear Incandescent Lamp 116009 R0 Ribbon Cable Pinout, 10 Conductor Cables, Nos. 1 thru 4 116010 R0 Ribbon Cable Pinout, 10 Conductor Cables, Nos. 5 thru 8 116011 R0 Ribbon Cable Pinout, 10 Conductor Cables, Nos. 9 thru 12 116012 R0 Home Location Definition Panel Commands 116013 R0 NI-DAQ Control Panel Configuration Settings in Macintosh Computer 116014 R0 Edge Connector for Valve Board 116015 R0 Switch Settings Diagram for SX-6 Controller