Genome Technology Center

Flow Through Micro-Centrifuge

Flow Through Micro-Centrifuge We have recently developed a novel technology to increase throughput and flexibility of protocols that require centrifugation.

Regular centrifugation, though potentially automatable, remains too unwieldy and slow a process for high throughput instrumentation. Filter based protocols can replace most but not all centrifugation steps and can be expensive due to the cost of the disposables involved. The flow through microcentrifuge (FTMC) is a high speed centrifugation system that is designed to be easily incorporated into automated instrumentation. The FTMC consists of a miniature, air driven high speed rotor capable of revolving around its axis at up to a few hundred thousand RPM. The rotor has a hollow, double conical inner profile into which sample is injected. The sample is spun with the rotor and particles pellet on the inner surface of the rotor. When the rotor is stopped, the supernatant drips out the bottom orifice.

There are two unique advantages to this technology. First, the rotors are less than 9mm in diameter and can therefore be arrayed in a standard 96 well plate format. Second, the flow-through method of sample handling makes sample injection and retrieval inherently easy to automate with few moving parts.

We have designed the rotors to allow rotation in both directions. This allows us to resuspend pellets in a small amount of buffer by rapidly changing rotation direction. Also, reagents can be mixed within the rotor by this same scheme. As a result, several protocol steps can often be performed without removing the sample from the rotor.

Contact the Stanford Office of Technology Licensing for more information on this instrument. (Docket # 97-116)

This instrument was licensed to GeneMachines (acquired by Genomic Solutions).

Spinning Test Rotor
Spinning Test Rotor
8-Channel Prototype
8-Channel Prototype
Titanium Rotor
Titanium Rotor
96-Channel Prototype
96-Channel Prototype
Operational Diagram
Operational Diagram
Another Picture
Another Image

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