Genome Technology Center

A New Human Transcriptome Array for Clinical Research

project logo We have designed a comprehensive high-density oligonucleotide array of human transcriptome (GG-H1 array) for high-throughput, multiplexed, and cost effective analyses of patient samples for the biological basis of diseases. This array enables comprehensive examination of multiple mechanisms human cells use to regulate transcriptome in response to diseases including improved analysis of gene expression with completely updated gene annotations and carefully redesigned probes, genome-wide quantitation of gene isoforms and identification of alternative splicing, coding and UTR SNP detection and allele specific expression analysis, examination of non-coding transcription and antisense expression, and the analyses of small RNAs.

Since many of the components on the new array have not been systematically studied in human before, computational methods are not yet available. In this project, therefore, we propose to develop statistical methods and bioinformatic tools for the analyses of the new information, and conduct testing experiments in selected clinical problems to evaluate the contributions of different parts of transcriptome regulation in human health and disease.

Personnel

SGTC
Wenzhong Xiao
Weihong Xu
Junhee Seok
Michael Mindrinos
Erik Miller
Julie Wilhelmy
Ronald W. Davis

Dept. of Statistics, Stanford University
Yi Xing
Hui Jiang
Karen Kapur
Dave Hiller
Zhegqing Ouyang
Wing Wong

Depts. of Pathology & Genetics, Stanford University
Weng-Onn Lui
Andrew Z. Fire

Affymetrix
Anyhony Schweitzer
Tyson Clark
Malek Faham
John Blume

Impact/Significance

Accomplishments

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